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• W2913794927 endingPage "3381" @default.
• W2913794927 startingPage "3374" @default.
• W2913794927 abstract "As an investigative tool, live-cell imaging requires superior probe design to guarantee imaging quality and data validity. The ability to simultaneously address the robustness, sensitivity, and consistency issues in a single-assay system is highly desired, but it remains a largely unsolved challenge. We describe herein a probe-design strategy called a nanoamplicon comparator (NAC) and demonstrate its proof-of-concept utility in intracellular microRNA (miRNA) imaging. This novel designer architecture builds upon spherical nucleic acids (SNAs) for robustness, catalytic hairpin assembly (CHA) for sensitivity, and upconversion nanoparticles (UNPs) for consistency. A catalytic circuit comprising a UNP−hairpin-DNA (UNP-HDNA) conjugate and a hairpin-DNA−organic-fluorophore (HDNA-F) conjugate as probe responds to target miRNA and generates the UNP-HDNA−HDNA-F complex as an NAC for quantitative UNP-to-organic-fluorophore-luminescence-resonance-energy-transfer (LRET) imaging against a native UNP-emission reference channel. An imaging application with miR21 shows the ability to monitor miRNA-expression levels across different cell lines and under an external stimulus." @default.
• W2913794927 created "2019-02-21" @default.
• W2913794927 creator A5003317656 @default.
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• W2913794927 date "2019-02-08" @default.
• W2913794927 modified "2023-10-18" @default.
• W2913794927 title "Nanoamplicon Comparator for Live-Cell MicroRNA Imaging" @default.
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• W2913794927 doi "https://doi.org/10.1021/acs.analchem.8b04661" @default.
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• W2913794927 hasPublicationYear "2019" @default.
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