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• W2913842227 abstract "Abstract Leptospirosis is a worldwide zoonosis caused by pathogenic bacteria of the genus Leptospira , which also includes free-living saprophyte strains. Many aspects of leptospiral basic biology and virulence mechanisms remain unexplored mainly due to the lack of effective genetic tools available for these bacteria. Recently, the type II CRISPR/Cas system from Streptococcus pyogenes has been widely used as an efficient genome engineering tool in bacteria by inducing double-strand breaks (DSBs) in the desired genomic targets caused by an RNA-guided DNA endonuclease called Cas9, and the DSB repair associated machinery. In the present work, plasmids expressing heterologous S. pyogenes Cas9 in L. biflexa cells were generated, and the enzyme could be expressed with no apparent toxicity to leptospiral cells. However, L. biflexa cells were unable to repair RNA-guided Cas9-induced DSBs. Thus, we used a catalytically dead Cas9 (dCas9) to obtain gene silencing rather than disruption, in a strategy called CRISPR interference (CRISPRi). We demonstrated complete gene silencing in L. biflexa cells when both dCas9 and single-guide RNA (sgRNA) targeting the coding strand of the β-galactosidase gene were expressed simultaneously. Furthermore, when the system was applied for silencing the dnaK gene, no colonies were recovered, indicating that DnaK protein is essential in Leptospira . In addition, flagellar motor switch FliG gene silencing resulted in reduced bacterial motility. To the best of our knowledge, this is the first work applying the CRISPRi system in Leptospira and spirochetes in general, expanding the tools available for understanding leptospiral biology." @default.
• W2913842227 created "2019-02-21" @default.
• W2913842227 creator A5017002489 @default.
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• W2913842227 creator A5085588413 @default.
• W2913842227 date "2019-02-12" @default.
• W2913842227 modified "2023-09-30" @default.
• W2913842227 title "Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in Leptospira" @default.
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• W2913842227 cites W1589018765 @default.
• W2913842227 cites W1813190012 @default.
• W2913842227 cites W1879384335 @default.
• W2913842227 cites W1916572066 @default.
• W2913842227 cites W1927180069 @default.
• W2913842227 cites W1933893097 @default.
• W2913842227 cites W1966797634 @default.
• W2913842227 cites W1967284543 @default.
• W2913842227 cites W1968709514 @default.
• W2913842227 cites W1970776621 @default.
• W2913842227 cites W1974127479 @default.
• W2913842227 cites W1975096626 @default.
• W2913842227 cites W1978094899 @default.
• W2913842227 cites W1994145955 @default.
• W2913842227 cites W2001845502 @default.
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• W2913842227 cites W2004730785 @default.
• W2913842227 cites W2008578499 @default.
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• W2913842227 cites W2043369680 @default.
• W2913842227 cites W2045435533 @default.
• W2913842227 cites W2058336786 @default.
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• W2913842227 cites W2096771207 @default.
• W2913842227 cites W2103764811 @default.
• W2913842227 cites W2107831820 @default.
• W2913842227 cites W2107870779 @default.
• W2913842227 cites W2112444085 @default.
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• W2913842227 cites W2120263648 @default.
• W2913842227 cites W2120932131 @default.
• W2913842227 cites W2122663726 @default.
• W2913842227 cites W2124215787 @default.
• W2913842227 cites W2125433188 @default.
• W2913842227 cites W2125889243 @default.
• W2913842227 cites W2128112204 @default.
• W2913842227 cites W2131122061 @default.
• W2913842227 cites W2133908827 @default.
• W2913842227 cites W2135517691 @default.
• W2913842227 cites W2136267644 @default.
• W2913842227 cites W2155208047 @default.
• W2913842227 cites W2157205823 @default.
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• W2913842227 cites W2162198166 @default.
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• W2913842227 cites W2168460045 @default.
• W2913842227 cites W2169244146 @default.
• W2913842227 cites W2233409659 @default.
• W2913842227 cites W2312592272 @default.
• W2913842227 cites W2341319313 @default.
• W2913842227 cites W2397457481 @default.
• W2913842227 cites W2407174002 @default.
• W2913842227 cites W2410060212 @default.
• W2913842227 cites W2469203838 @default.
• W2913842227 cites W2575268672 @default.
• W2913842227 cites W2605735428 @default.
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• W2913842227 doi "https://doi.org/10.1038/s41598-018-37949-x" @default.
• W2913842227 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6372684" @default.
• W2913842227 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30755626" @default.
• W2913842227 hasPublicationYear "2019" @default.
• W2913842227 type Work @default.
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• W2913842227 hasAuthorship W2913842227A5017002489 @default.
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