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- W2913883391 abstract "We previously demonstrated that altering extracellular sodium (Na o ) and calcium (Ca o ) can modulate a form of electrical communication between cardiomyocytes termed “ephaptic coupling” (EpC), especially during loss of gap junction coupling. We hypothesized that altering Na o and Ca o modulates conduction velocity (CV) and arrhythmic burden during ischemia. Electrophysiology was quantified by optically mapping Langendorff-perfused guinea pig ventricles with modified Na o (147 or 155 mM) and Ca o (1.25 or 2.0 mM) during 30 min of simulated metabolic ischemia (pH 6.5, anoxia, aglycemia). Gap junction-adjacent perinexal width ( W P ), a candidate cardiac ephapse, and connexin (Cx)43 protein expression and Cx43 phosphorylation at S368 were quantified by transmission electron microscopy and Western immunoblot analysis, respectively. Metabolic ischemia slowed CV in hearts perfused with 147 mM Na o and 2.0 mM Ca o ; however, theoretically increasing EpC with 155 mM Na o was arrhythmogenic, and CV could not be measured. Reducing Ca o to 1.25 mM expanded W P , as expected during ischemia, consistent with reduced EpC, but attenuated CV slowing while delaying arrhythmia onset. These results were further supported by osmotically reducing W P with albumin, which exacerbated CV slowing and increased early arrhythmias during ischemia, whereas mannitol expanded W P , permitted conduction, and delayed the onset of arrhythmias. Cx43 expression patterns during the various interventions insufficiently correlated with observed CV changes and arrhythmic burden. In conclusion, decreasing perfusate calcium during metabolic ischemia enhances perinexal expansion, attenuates conduction slowing, and delays arrhythmias. Thus, perinexal expansion may be cardioprotective during metabolic ischemia. NEW & NOTEWORTHY This study demonstrates, for the first time, that modulating perfusate ion composition can alter cardiac electrophysiology during simulated metabolic ischemia." @default.
- W2913883391 created "2019-02-21" @default.
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- W2913883391 date "2019-04-01" @default.
- W2913883391 modified "2023-10-17" @default.
- W2913883391 title "Modulating cardiac conduction during metabolic ischemia with perfusate sodium and calcium in guinea pig hearts" @default.
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- W2913883391 cites W1974811499 @default.
- W2913883391 cites W1978812569 @default.
- W2913883391 cites W1980092173 @default.
- W2913883391 cites W1985496482 @default.
- W2913883391 cites W1988674900 @default.
- W2913883391 cites W1991615148 @default.
- W2913883391 cites W1992079042 @default.
- W2913883391 cites W1994221403 @default.
- W2913883391 cites W1995907435 @default.
- W2913883391 cites W1996445973 @default.
- W2913883391 cites W1998896690 @default.
- W2913883391 cites W2001262069 @default.
- W2913883391 cites W2005568437 @default.
- W2913883391 cites W2019326075 @default.
- W2913883391 cites W2031426329 @default.
- W2913883391 cites W2032088191 @default.
- W2913883391 cites W2033620037 @default.
- W2913883391 cites W2037521507 @default.
- W2913883391 cites W2037640251 @default.
- W2913883391 cites W2043555103 @default.
- W2913883391 cites W2044650128 @default.
- W2913883391 cites W2044788445 @default.
- W2913883391 cites W2047844150 @default.
- W2913883391 cites W2050627314 @default.
- W2913883391 cites W2062570493 @default.
- W2913883391 cites W2073521043 @default.
- W2913883391 cites W2073773271 @default.
- W2913883391 cites W2084001565 @default.
- W2913883391 cites W2084265884 @default.
- W2913883391 cites W2092312267 @default.
- W2913883391 cites W2092513986 @default.
- W2913883391 cites W2093043176 @default.
- W2913883391 cites W2108790161 @default.
- W2913883391 cites W2110614144 @default.
- W2913883391 cites W2111880897 @default.
- W2913883391 cites W2113153848 @default.
- W2913883391 cites W2115156315 @default.
- W2913883391 cites W2119568772 @default.
- W2913883391 cites W2120303052 @default.
- W2913883391 cites W2122202328 @default.
- W2913883391 cites W2124453111 @default.
- W2913883391 cites W2133363206 @default.
- W2913883391 cites W2136290454 @default.
- W2913883391 cites W2151563090 @default.
- W2913883391 cites W2152543475 @default.
- W2913883391 cites W2154911363 @default.
- W2913883391 cites W2158899431 @default.
- W2913883391 cites W2162541315 @default.
- W2913883391 cites W2162890160 @default.
- W2913883391 cites W2163869625 @default.
- W2913883391 cites W2184066959 @default.
- W2913883391 cites W2245266743 @default.
- W2913883391 cites W2290553761 @default.
- W2913883391 cites W2301058553 @default.
- W2913883391 cites W2420880898 @default.
- W2913883391 cites W2579869531 @default.
- W2913883391 cites W2581605534 @default.
- W2913883391 cites W2619225810 @default.
- W2913883391 cites W2758583321 @default.
- W2913883391 cites W2886411329 @default.
- W2913883391 cites W289421133 @default.
- W2913883391 cites W2897575226 @default.
- W2913883391 cites W3023737981 @default.
- W2913883391 cites W4293084779 @default.
- W2913883391 cites W642235081 @default.
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- W2913883391 doi "https://doi.org/10.1152/ajpheart.00083.2018" @default.
- W2913883391 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6483020" @default.
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- W2913883391 hasPublicationYear "2019" @default.
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