Matches in SemOpenAlex for { <https://semopenalex.org/work/W2913956407> ?p ?o ?g. }
- W2913956407 endingPage "399" @default.
- W2913956407 startingPage "391" @default.
- W2913956407 abstract "Aqueous two-phase partitioning system (ATPS) was used to extract and purify catalase from Bacillus pumilus. The system parameters for effective purification of catalase were optimized. The best catalase recovery (123%) with a 4.6-fold purification was obtained in the bottom phase of ATPS including the mixture of 15% (w/w) PEG4000, 10% (w/w) Na2SO4 and 3% (w/w) NaCl at pH 5.0. The purified enzyme was characterized regarding its activity and stability. The highest enzyme activity was observed at pH 7.0 and 37 °C on hydrogen peroxide. The enzyme was quite stable at temperatures between 30 and 55 °C and a pH range of 7.0–9.0. The Km and Vmax values were determined from Lineweaver–Burk plot as 11 mM and 1667 µmole ml−1 min−1, respectively. Overall, it can be said that ATPS is a rapid, reasonable, straightforward and cost-effective process for catalase purification in comparison to the chromatographic methods." @default.
- W2913956407 created "2019-02-21" @default.
- W2913956407 creator A5022664471 @default.
- W2913956407 creator A5091599596 @default.
- W2913956407 date "2019-02-15" @default.
- W2913956407 modified "2023-10-01" @default.
- W2913956407 title "Partial characterization of <i>Bacillus pumilus</i> catalase partitioned in poly(ethylene glycol)/sodium sulfate aqueous two-phase systems" @default.
- W2913956407 cites W1516125561 @default.
- W2913956407 cites W1936422260 @default.
- W2913956407 cites W1960929562 @default.
- W2913956407 cites W1966958372 @default.
- W2913956407 cites W1970956141 @default.
- W2913956407 cites W1979041090 @default.
- W2913956407 cites W1979739219 @default.
- W2913956407 cites W1981264591 @default.
- W2913956407 cites W1984470315 @default.
- W2913956407 cites W1984474731 @default.
- W2913956407 cites W1985992238 @default.
- W2913956407 cites W1988981787 @default.
- W2913956407 cites W1998727644 @default.
- W2913956407 cites W2005463277 @default.
- W2913956407 cites W2009441081 @default.
- W2913956407 cites W2012356106 @default.
- W2913956407 cites W2022072246 @default.
- W2913956407 cites W2023099428 @default.
- W2913956407 cites W2026863324 @default.
- W2913956407 cites W2028102390 @default.
- W2913956407 cites W2028543209 @default.
- W2913956407 cites W2035782381 @default.
- W2913956407 cites W2040549825 @default.
- W2913956407 cites W2044853699 @default.
- W2913956407 cites W2046846368 @default.
- W2913956407 cites W2053901597 @default.
- W2913956407 cites W2054745293 @default.
- W2913956407 cites W2054883929 @default.
- W2913956407 cites W2061348615 @default.
- W2913956407 cites W2062638036 @default.
- W2913956407 cites W2064956451 @default.
- W2913956407 cites W2077569587 @default.
- W2913956407 cites W2080592996 @default.
- W2913956407 cites W2088333517 @default.
- W2913956407 cites W2088693581 @default.
- W2913956407 cites W2100837269 @default.
- W2913956407 cites W2102054380 @default.
- W2913956407 cites W2127826037 @default.
- W2913956407 cites W2144486224 @default.
- W2913956407 cites W2160107281 @default.
- W2913956407 cites W2520872371 @default.
- W2913956407 cites W2546625455 @default.
- W2913956407 cites W2755820371 @default.
- W2913956407 cites W2764251403 @default.
- W2913956407 cites W2770895461 @default.
- W2913956407 cites W2797497966 @default.
- W2913956407 cites W2895375910 @default.
- W2913956407 cites W3091364708 @default.
- W2913956407 cites W4293247451 @default.
- W2913956407 doi "https://doi.org/10.1080/10826068.2019.1573197" @default.
- W2913956407 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30767698" @default.
- W2913956407 hasPublicationYear "2019" @default.
- W2913956407 type Work @default.
- W2913956407 sameAs 2913956407 @default.
- W2913956407 citedByCount "3" @default.
- W2913956407 countsByYear W29139564072020 @default.
- W2913956407 countsByYear W29139564072022 @default.
- W2913956407 countsByYear W29139564072023 @default.
- W2913956407 crossrefType "journal-article" @default.
- W2913956407 hasAuthorship W2913956407A5022664471 @default.
- W2913956407 hasAuthorship W2913956407A5091599596 @default.
- W2913956407 hasConcept C13965031 @default.
- W2913956407 hasConcept C178790620 @default.
- W2913956407 hasConcept C181199279 @default.
- W2913956407 hasConcept C184651966 @default.
- W2913956407 hasConcept C185592680 @default.
- W2913956407 hasConcept C2777516009 @default.
- W2913956407 hasConcept C2778187402 @default.
- W2913956407 hasConcept C2778979269 @default.
- W2913956407 hasConcept C43617362 @default.
- W2913956407 hasConcept C523546767 @default.
- W2913956407 hasConcept C533411734 @default.
- W2913956407 hasConcept C54355233 @default.
- W2913956407 hasConcept C55493867 @default.
- W2913956407 hasConcept C86803240 @default.
- W2913956407 hasConceptScore W2913956407C13965031 @default.
- W2913956407 hasConceptScore W2913956407C178790620 @default.
- W2913956407 hasConceptScore W2913956407C181199279 @default.
- W2913956407 hasConceptScore W2913956407C184651966 @default.
- W2913956407 hasConceptScore W2913956407C185592680 @default.
- W2913956407 hasConceptScore W2913956407C2777516009 @default.
- W2913956407 hasConceptScore W2913956407C2778187402 @default.
- W2913956407 hasConceptScore W2913956407C2778979269 @default.
- W2913956407 hasConceptScore W2913956407C43617362 @default.
- W2913956407 hasConceptScore W2913956407C523546767 @default.
- W2913956407 hasConceptScore W2913956407C533411734 @default.
- W2913956407 hasConceptScore W2913956407C54355233 @default.
- W2913956407 hasConceptScore W2913956407C55493867 @default.
- W2913956407 hasConceptScore W2913956407C86803240 @default.
- W2913956407 hasIssue "4" @default.
- W2913956407 hasLocation W29139564071 @default.