FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2914109673> ?p ?o ?g. }

• W2914109673 abstract "Few characteristics are more important to a bacterium than the substrates it consumes. It is hard to identify what substrates are consumed by bacteria in natural communities, however, because most bacteria have not been cultured. In this study, we developed a method that uses fluorescent substrate analogs, cell sorting, and DNA sequencing to identify substrates taken up by bacteria. We deployed this method using 2[N-(7-nitrobenz-2-oxa-1,2-diaxol-4-yl)amino]-2-deoxyglucose (2-NBDG), a fluorescent glucose analog, and bacteria of the bovine rumen. This method revealed over 40 different bacteria (amplicon sequence variants [ASVs]) from the rumen that take up glucose. Nearly half of these ASVs represent previously uncultured bacteria. We attempted to grow these ASVs on agar media, and we confirmed that nearly two-thirds resisted culture. In coculture experiments, the fluorescent label of 2-NBDG was not transferred to nontarget bacteria by cross-feeding. Because it is not affected by cross-feeding, our method has an advantage over stable isotope probing. Though we focus on glucose, many substrates can be labeled with the fluorophore NBD. Our method represents a new paradigm for identifying substrates used by uncultured bacteria. It will help delineate the niche of bacteria in their environment.IMPORTANCE We introduce a method for identifying what substrates are consumed by bacteria in natural communities. Our method offers significant improvement over existing methods for studying this characteristic. Our method uses a fluorescently labeled substrate which clearly labels target bacteria (glucose consumers in our case). Previous methods use isotope-labeled substrates, which are notorious for off-target labeling (due to cross-feeding of labeled metabolites). Our method can be deployed with a variety of substrates and microbial communities. It represents a major advance in connecting bacteria to the substrates they take up." @default.
• W2914109673 created "2019-02-21" @default.
• W2914109673 creator A5000601534 @default.
• W2914109673 creator A5015344066 @default.
• W2914109673 creator A5060108600 @default.
• W2914109673 creator A5079646564 @default.
• W2914109673 creator A5087326457 @default.
• W2914109673 creator A5089248847 @default.
• W2914109673 date "2019-04-01" @default.
• W2914109673 modified "2023-10-17" @default.
• W2914109673 title "Use of a Fluorescent Analog of Glucose (2-NBDG) To Identify Uncultured Rumen Bacteria That Take Up Glucose" @default.
• W2914109673 cites W1057466098 @default.
• W2914109673 cites W1513168266 @default.
• W2914109673 cites W1519909963 @default.
• W2914109673 cites W1635555518 @default.
• W2914109673 cites W1760389142 @default.
• W2914109673 cites W1801704971 @default.
• W2914109673 cites W1900899030 @default.
• W2914109673 cites W1950130060 @default.
• W2914109673 cites W1971433113 @default.
• W2914109673 cites W1983182864 @default.
• W2914109673 cites W1994759929 @default.
• W2914109673 cites W2011717324 @default.
• W2914109673 cites W2017681432 @default.
• W2914109673 cites W2024648928 @default.
• W2914109673 cites W2031872005 @default.
• W2914109673 cites W2034285706 @default.
• W2914109673 cites W2042473229 @default.
• W2914109673 cites W2056190129 @default.
• W2914109673 cites W2060520177 @default.
• W2914109673 cites W2060797027 @default.
• W2914109673 cites W2063455022 @default.
• W2914109673 cites W2064837899 @default.
• W2914109673 cites W2072970694 @default.
• W2914109673 cites W2082891742 @default.
• W2914109673 cites W2086522442 @default.
• W2914109673 cites W2086999280 @default.
• W2914109673 cites W2095158284 @default.
• W2914109673 cites W2098584636 @default.
• W2914109673 cites W2102138046 @default.
• W2914109673 cites W2107828561 @default.
• W2914109673 cites W2128601422 @default.
• W2914109673 cites W2129554829 @default.
• W2914109673 cites W2138640890 @default.
• W2914109673 cites W2140880990 @default.
• W2914109673 cites W2147378425 @default.
• W2914109673 cites W2152885278 @default.
• W2914109673 cites W2156521321 @default.
• W2914109673 cites W2166123725 @default.
• W2914109673 cites W2168104781 @default.
• W2914109673 cites W2318211804 @default.
• W2914109673 cites W2338601319 @default.
• W2914109673 cites W2345813655 @default.
• W2914109673 cites W2401404581 @default.
• W2914109673 cites W2465488908 @default.
• W2914109673 cites W2513506562 @default.
• W2914109673 cites W2530896378 @default.
• W2914109673 cites W2549670165 @default.
• W2914109673 cites W2559588208 @default.
• W2914109673 cites W2753961085 @default.
• W2914109673 cites W2755042773 @default.
• W2914109673 cites W2757805629 @default.
• W2914109673 cites W2766358903 @default.
• W2914109673 cites W2792729061 @default.
• W2914109673 cites W2950785362 @default.
• W2914109673 cites W2950921215 @default.
• W2914109673 cites W4238709006 @default.
• W2914109673 doi "https://doi.org/10.1128/aem.03018-18" @default.
• W2914109673 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6585498" @default.
• W2914109673 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30709823" @default.
• W2914109673 hasPublicationYear "2019" @default.
• W2914109673 type Work @default.
• W2914109673 sameAs 2914109673 @default.
• W2914109673 citedByCount "10" @default.
• W2914109673 countsByYear W29141096732020 @default.
• W2914109673 countsByYear W29141096732021 @default.
• W2914109673 countsByYear W29141096732022 @default.
• W2914109673 crossrefType "journal-article" @default.
• W2914109673 hasAuthorship W2914109673A5000601534 @default.
• W2914109673 hasAuthorship W2914109673A5015344066 @default.
• W2914109673 hasAuthorship W2914109673A5060108600 @default.
• W2914109673 hasAuthorship W2914109673A5079646564 @default.
• W2914109673 hasAuthorship W2914109673A5087326457 @default.
• W2914109673 hasAuthorship W2914109673A5089248847 @default.
• W2914109673 hasBestOaLocation W29141096731 @default.
• W2914109673 hasConcept C100544194 @default.
• W2914109673 hasConcept C104317684 @default.
• W2914109673 hasConcept C121332964 @default.
• W2914109673 hasConcept C175605896 @default.
• W2914109673 hasConcept C18903297 @default.
• W2914109673 hasConcept C2777289219 @default.
• W2914109673 hasConcept C2777624702 @default.
• W2914109673 hasConcept C2778617687 @default.
• W2914109673 hasConcept C2778660310 @default.
• W2914109673 hasConcept C49105822 @default.
• W2914109673 hasConcept C523546767 @default.
• W2914109673 hasConcept C54355233 @default.
• W2914109673 hasConcept C55493867 @default.
• W2914109673 hasConcept C62520636 @default.
• W2914109673 hasConcept C70262065 @default.

• next