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• W2914396472 endingPage "179" @default.
• W2914396472 startingPage "171" @default.
• W2914396472 abstract "The excessive cell death rate caused by electrofusion with unipolar pulses (UPs) has been a bottleneck to increasing cell fusion efficiency in monoclonal antibody technology. Several studies have confirmed that compared with UPs, bipolar pulses (BPs) with microsecond pulse widths can increase electropermeabilization while reducing cell death. Given these characteristics, BPs were used to increase cell fusion efficiency in this study. Cell staining and hybridoma culture experiments were performed using SP2/0 mouse myeloma cells and lymphocytes. Based on the equal energy principle, UPs and BPs were delivered to electrodes at a distance of 3.81 mm, with electric field intensities ranging from 2 kV/cm to 3 kV/cm and pulse duration of 40 μs for the UPs and 20–20 μs for the BPs. The results of cell staining experiments showed that cell fusion efficiency was 3-fold greater with BPs than with UPs. Similarly, the results of the hybridoma culture experiments showed that the hybridoma yields were 0.26‰ and 0.23‰ (2.5 kV/cm and 3 kV/cm, respectively) in the UP groups and increased to 0.46‰ and 0.35‰ in the BP groups. Taken together, the results show that the efficiency of heterologous cell fusion can be greatly increased if BPs are used instead of the commonly applied UPs. This study may provide a promising method for monoclonal antibody technology." @default.
• W2914396472 created "2019-02-21" @default.
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• W2914396472 date "2019-06-01" @default.
• W2914396472 modified "2023-10-18" @default.
• W2914396472 title "Electrofusion by a bipolar pulsed electric field: Increased cell fusion efficiency for monoclonal antibody production" @default.
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• W2914396472 doi "https://doi.org/10.1016/j.bioelechem.2019.02.007" @default.
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• W2914396472 hasPublicationYear "2019" @default.
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