Matches in SemOpenAlex for { <https://semopenalex.org/work/W2914443620> ?p ?o ?g. }
- W2914443620 abstract "Manganese (Mn)-associated neurotoxicity has been well recognized. However, Mn is also an essential nutrient to maintain physiological function. Our previous study of human neuroblastoma SH-SY5Y cells showed that Mn treatment comparable to physiological and toxicological concentrations in human brain resulted in different mitochondrial responses, yet cellular metabolic responses associated with such different outcomes remain uncharacterized. Herein, SH-SY5Y cells were examined for metabolic responses discriminated by physiological and toxicological levels of Mn using high-resolution metabolomics (HRM). Before performing HRM, we examined Mn dose (from 0 to100 μM) and time effects on cell death. Although we did not observe any immediate cell death after 5 h exposure to any of the Mn concentrations assessed (0-100 μM), cell loss was present after a 24-h recovery period in cultures treated with Mn ≥ 50 μM. Exposure to Mn for 5 h resulted in a wide range of changes in cellular metabolism including amino acids (AA), neurotransmitters, energy, and fatty acids metabolism. Adaptive responses at 10 μM showed increases in neuroprotective AA metabolites (creatine, phosphocreatine, phosphoserine). A 5-h exposure to 100 µM Mn, a time before any cell death occurred, resulted in decreases in energy and fatty acid metabolites (hexose-1,6 bisphosphate, acyl carnitines). The results show that adjustments in AA metabolism occur in response to Mn that does not cause cell death while disruption in energy and fatty acid metabolism occur in response to Mn that results in subsequent cell death. The present study establishes utility for metabolomics analyses to discriminate adaptive and toxic molecular responses in a human in vitro cellular model that could be exploited in evaluation of Mn toxicity." @default.
- W2914443620 created "2019-02-21" @default.
- W2914443620 creator A5019994023 @default.
- W2914443620 creator A5023117638 @default.
- W2914443620 creator A5027711144 @default.
- W2914443620 creator A5027850301 @default.
- W2914443620 creator A5032746548 @default.
- W2914443620 creator A5035904189 @default.
- W2914443620 creator A5037374190 @default.
- W2914443620 creator A5085279437 @default.
- W2914443620 date "2019-01-31" @default.
- W2914443620 modified "2023-10-12" @default.
- W2914443620 title "Metabolomic Responses to Manganese Dose in SH-SY5Y Human Neuroblastoma Cells" @default.
- W2914443620 cites W1456625250 @default.
- W2914443620 cites W1504925078 @default.
- W2914443620 cites W154045452 @default.
- W2914443620 cites W1565043637 @default.
- W2914443620 cites W162471263 @default.
- W2914443620 cites W1947475203 @default.
- W2914443620 cites W1965963335 @default.
- W2914443620 cites W1968547755 @default.
- W2914443620 cites W1969195984 @default.
- W2914443620 cites W1970369074 @default.
- W2914443620 cites W1977560462 @default.
- W2914443620 cites W1981450138 @default.
- W2914443620 cites W1988236146 @default.
- W2914443620 cites W1990623139 @default.
- W2914443620 cites W2001311316 @default.
- W2914443620 cites W2012660994 @default.
- W2914443620 cites W2023074330 @default.
- W2914443620 cites W2024495771 @default.
- W2914443620 cites W2024612421 @default.
- W2914443620 cites W2025944550 @default.
- W2914443620 cites W2030954436 @default.
- W2914443620 cites W2032047739 @default.
- W2914443620 cites W2032984035 @default.
- W2914443620 cites W2035040637 @default.
- W2914443620 cites W2036069083 @default.
- W2914443620 cites W2039142811 @default.
- W2914443620 cites W2043430676 @default.
- W2914443620 cites W2058375303 @default.
- W2914443620 cites W2061268491 @default.
- W2914443620 cites W2063028213 @default.
- W2914443620 cites W2071808643 @default.
- W2914443620 cites W2076682588 @default.
- W2914443620 cites W2076989525 @default.
- W2914443620 cites W2084375915 @default.
- W2914443620 cites W2093898849 @default.
- W2914443620 cites W2099218280 @default.
- W2914443620 cites W2100659128 @default.
- W2914443620 cites W2109669266 @default.
- W2914443620 cites W2111613263 @default.
- W2914443620 cites W2130319566 @default.
- W2914443620 cites W2134466886 @default.
- W2914443620 cites W2137976898 @default.
- W2914443620 cites W2138754505 @default.
- W2914443620 cites W2143205261 @default.
- W2914443620 cites W2143598546 @default.
- W2914443620 cites W2156769592 @default.
- W2914443620 cites W2165090609 @default.
- W2914443620 cites W2169505993 @default.
- W2914443620 cites W2249880132 @default.
- W2914443620 cites W2283871706 @default.
- W2914443620 cites W2319744634 @default.
- W2914443620 cites W2322580831 @default.
- W2914443620 cites W2340606132 @default.
- W2914443620 cites W2411122904 @default.
- W2914443620 cites W2511867922 @default.
- W2914443620 cites W2518686384 @default.
- W2914443620 cites W2520222164 @default.
- W2914443620 cites W2526001849 @default.
- W2914443620 cites W2526986571 @default.
- W2914443620 cites W2529573043 @default.
- W2914443620 cites W2529620563 @default.
- W2914443620 cites W2559588208 @default.
- W2914443620 cites W2561291010 @default.
- W2914443620 cites W2585611321 @default.
- W2914443620 cites W2605148305 @default.
- W2914443620 cites W2614680488 @default.
- W2914443620 cites W2741564780 @default.
- W2914443620 cites W2755281951 @default.
- W2914443620 cites W2758551408 @default.
- W2914443620 cites W2778724589 @default.
- W2914443620 cites W2799537427 @default.
- W2914443620 cites W2950985821 @default.
- W2914443620 cites W839847231 @default.
- W2914443620 doi "https://doi.org/10.1093/toxsci/kfz028" @default.
- W2914443620 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6484887" @default.
- W2914443620 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30715528" @default.
- W2914443620 hasPublicationYear "2019" @default.
- W2914443620 type Work @default.
- W2914443620 sameAs 2914443620 @default.
- W2914443620 citedByCount "12" @default.
- W2914443620 countsByYear W29144436202019 @default.
- W2914443620 countsByYear W29144436202020 @default.
- W2914443620 countsByYear W29144436202021 @default.
- W2914443620 countsByYear W29144436202022 @default.
- W2914443620 countsByYear W29144436202023 @default.
- W2914443620 crossrefType "journal-article" @default.
- W2914443620 hasAuthorship W2914443620A5019994023 @default.