FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2914542817> ?p ?o ?g. }

• W2914542817 endingPage "182" @default.
• W2914542817 startingPage "174" @default.
• W2914542817 abstract "Abstract The encapsulated enzyme system by metal–organic frameworks exhibited great potential in pharmaceuticals, enzyme fuel cells, and biocatalysis. However, the enzymatic activity and overall catalytic efficiency are severely hampered due to their enzyme leaching and causal enzyme location. Herein, for the first time, horseradish peroxidase (HRP) and glucose oxidase (GOx) used as model enzymes were cross-linked by rationally designed DNA scaffold network, and the multienzyme system has been successfully encapsulated into zeolite imidazolate framework-8 (ZIF-8). The prepared encapsulation multienzyme can efficiently suppress enzyme leaching from the MOFs skeleton, and the leaching amount was more than 10-times lower than the traditional MOFs encapsulation strategy. The well-tailored proximity and colocalization of cascade enzymes by the DNA scaffold network obviously improved the overall catalytic efficiency, enzymatic activity, and kinetic performance. The Km and kcat/Km for encapsulation multienzyme was 13.8 mM and 3.47 s−1 mM−1, which was 0.27-fold lower and 9.9-fold higher than those of free enzymes, respectively, indicating a better substrate affinity. Furthermore, the multienzyme system exhibited excellent stability and reusability compared with free enzymes and control groups, which could preserve 95% initial activity at 50 °C after 75 min incubation and more than 87% of the original activity after 14 cycles. Finally, the immobilized multienzyme exhibited enhanced performance to detect low concentration of glucose (0.4 µM) with excellent selectivity. The outcome indicated that the encapsulated multienzyme could be extended for broad practical applications in biotechnology, biocatalysis, and biomedical engineering." @default.
• W2914542817 created "2019-02-21" @default.
• W2914542817 creator A5000074603 @default.
• W2914542817 creator A5020633369 @default.
• W2914542817 creator A5023014184 @default.
• W2914542817 creator A5031102018 @default.
• W2914542817 creator A5058291293 @default.
• W2914542817 creator A5084375841 @default.
• W2914542817 creator A5089822225 @default.
• W2914542817 date "2019-05-01" @default.
• W2914542817 modified "2023-09-23" @default.
• W2914542817 title "Construction of multiple enzyme metal–organic frameworks biocatalyst via DNA scaffold: A promising strategy for enzyme encapsulation" @default.
• W2914542817 cites W1001590327 @default.
• W2914542817 cites W1878403260 @default.
• W2914542817 cites W1914889505 @default.
• W2914542817 cites W1966172361 @default.
• W2914542817 cites W1981263132 @default.
• W2914542817 cites W1992658363 @default.
• W2914542817 cites W1998430793 @default.
• W2914542817 cites W2005518847 @default.
• W2914542817 cites W2005615084 @default.
• W2914542817 cites W2008686601 @default.
• W2914542817 cites W2018860363 @default.
• W2914542817 cites W2051739566 @default.
• W2914542817 cites W2058020286 @default.
• W2914542817 cites W2070814889 @default.
• W2914542817 cites W2079013207 @default.
• W2914542817 cites W2088389573 @default.
• W2914542817 cites W2102424174 @default.
• W2914542817 cites W2103031461 @default.
• W2914542817 cites W2107507270 @default.
• W2914542817 cites W2109135544 @default.
• W2914542817 cites W2113391704 @default.
• W2914542817 cites W2115269241 @default.
• W2914542817 cites W2126078170 @default.
• W2914542817 cites W2138393107 @default.
• W2914542817 cites W2147516046 @default.
• W2914542817 cites W2161278159 @default.
• W2914542817 cites W2163535530 @default.
• W2914542817 cites W2164697359 @default.
• W2914542817 cites W2275766475 @default.
• W2914542817 cites W2285098014 @default.
• W2914542817 cites W2327663231 @default.
• W2914542817 cites W2331326812 @default.
• W2914542817 cites W2331640858 @default.
• W2914542817 cites W2346463032 @default.
• W2914542817 cites W2494277124 @default.
• W2914542817 cites W2517659397 @default.
• W2914542817 cites W2562443457 @default.
• W2914542817 cites W2564356052 @default.
• W2914542817 cites W2608376917 @default.
• W2914542817 cites W2613420214 @default.
• W2914542817 cites W2677847820 @default.
• W2914542817 cites W2761082931 @default.
• W2914542817 cites W2796452276 @default.
• W2914542817 cites W2809542428 @default.
• W2914542817 doi "https://doi.org/10.1016/j.cej.2019.01.138" @default.
• W2914542817 hasPublicationYear "2019" @default.
• W2914542817 type Work @default.
• W2914542817 sameAs 2914542817 @default.
• W2914542817 citedByCount "65" @default.
• W2914542817 countsByYear W29145428172019 @default.
• W2914542817 countsByYear W29145428172020 @default.
• W2914542817 countsByYear W29145428172021 @default.
• W2914542817 countsByYear W29145428172022 @default.
• W2914542817 countsByYear W29145428172023 @default.
• W2914542817 crossrefType "journal-article" @default.
• W2914542817 hasAuthorship W2914542817A5000074603 @default.
• W2914542817 hasAuthorship W2914542817A5020633369 @default.
• W2914542817 hasAuthorship W2914542817A5023014184 @default.
• W2914542817 hasAuthorship W2914542817A5031102018 @default.
• W2914542817 hasAuthorship W2914542817A5058291293 @default.
• W2914542817 hasAuthorship W2914542817A5084375841 @default.
• W2914542817 hasAuthorship W2914542817A5089822225 @default.
• W2914542817 hasConcept C127413603 @default.
• W2914542817 hasConcept C136229726 @default.
• W2914542817 hasConcept C150394285 @default.
• W2914542817 hasConcept C154881586 @default.
• W2914542817 hasConcept C161790260 @default.
• W2914542817 hasConcept C171250308 @default.
• W2914542817 hasConcept C178790620 @default.
• W2914542817 hasConcept C179366358 @default.
• W2914542817 hasConcept C181199279 @default.
• W2914542817 hasConcept C183696295 @default.
• W2914542817 hasConcept C185592680 @default.
• W2914542817 hasConcept C191503008 @default.
• W2914542817 hasConcept C192562407 @default.
• W2914542817 hasConcept C21951064 @default.
• W2914542817 hasConcept C31258907 @default.
• W2914542817 hasConcept C33633552 @default.
• W2914542817 hasConcept C41008148 @default.
• W2914542817 hasConcept C552990157 @default.
• W2914542817 hasConcept C55493867 @default.
• W2914542817 hasConcept C81147070 @default.
• W2914542817 hasConcept C89429830 @default.
• W2914542817 hasConceptScore W2914542817C127413603 @default.
• W2914542817 hasConceptScore W2914542817C136229726 @default.
• W2914542817 hasConceptScore W2914542817C150394285 @default.

• next