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• W2914576668 startingPage "e0211192" @default.
• W2914576668 abstract "Adenovirus executes a finely tuned transcriptional program upon infection of a cell. To better understand the temporal dynamics of the viral transcriptional program we performed highly sensitive digital PCR on samples extracted from arrested human lung fibroblasts infected with human adenovirus 5 strain dl309. We show that the first transcript made from viral genomes is the virus associated non-coding RNA, in particular we detected abundant levels of virus associated RNA II four hours after infection. Activation of E1 and E4 occurred nearly simultaneously later in infection, followed by other early genes as well as late genes. Our study determined that genomes begin to replicate between 29 and 30 hours after infection. This study provides a comprehensive view of viral mRNA steady-state kinetics in arrested human cells using digital PCR." @default.
• W2914576668 created "2019-02-21" @default.
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• W2914576668 date "2019-01-24" @default.
• W2914576668 modified "2023-10-15" @default.
• W2914576668 title "Temporal dynamics of adenovirus 5 gene expression in normal human cells" @default.
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• W2914576668 doi "https://doi.org/10.1371/journal.pone.0211192" @default.
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