FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W291482005> ?p ?o ?g. }

• W291482005 endingPage "8" @default.
• W291482005 startingPage "94" @default.
• W291482005 abstract "Epidermal Langerhans' cells (LC) from human immunodeficiency virus type-1 (HIV-1)-infected patients harbour HIV-1 proviral DNA and RNA. In the present study, we investigated whether LC from epidermis of normal, HIV-seronegative subjects could be infected in vitro with HIV-1. Epidermal cells (EC) spontaneously detached from epidermal sheet cultures were enriched for LC (10-25% of CD1a+/CD4+ cells), deprived of contaminating T cells and then incubated with HIV-1IIIB. After 24 hr, purified LC and LC-depleted EC fractions were obtained by immunomagnetic separation. Polymerase chain reaction (PCR) analysis showed the presence of HIV-1 proviral DNA (gag) only in purified LC. In addition, LC-enriched EC, purified LC, LC-depleted EC or the non-permissive cell line, TF-1, the latter having being previously challenged with HIV-1IIIB for the same length of time as the EC, were co-cultivated with C8166 cells, and the co-cultures assessed for the presence of HIV DNA by PCR. Co-cultures of C8166 cells with purified LC or LC-enriched EC previously exposed to HIV-1IIIB exhibited a time-dependent increase in HIV proviral DNA. In contrast, PCR analysis of C8166 cells co-cultured with either LC-depleted EC or TF-1 cells gave negative results. Finally, C8166 cells co-cultured with HIV-infected LC formed syncytia, showed membrane budding and released numerous retroviral particles. The results indicate that LC from normal subjects can be infected in vitro with HIV and can transmit infection to myeloid cells. This in vitro model may help in understanding the regulation of HIV infection of LC." @default.
• W291482005 created "2016-06-24" @default.
• W291482005 creator A5009160829 @default.
• W291482005 creator A5009892820 @default.
• W291482005 creator A5013200935 @default.
• W291482005 creator A5016818733 @default.
• W291482005 creator A5029878487 @default.
• W291482005 creator A5058147541 @default.
• W291482005 creator A5064145324 @default.
• W291482005 creator A5067085999 @default.
• W291482005 creator A5067870640 @default.
• W291482005 creator A5087627289 @default.
• W291482005 date "1995-05-01" @default.
• W291482005 modified "2023-10-18" @default.
• W291482005 title "In vitro infection of human epidermal Langerhans' cells with HIV-1." @default.
• W291482005 cites W1272819057 @default.
• W291482005 cites W1486266992 @default.
• W291482005 cites W1508059507 @default.
• W291482005 cites W154086483 @default.
• W291482005 cites W1547706575 @default.
• W291482005 cites W1606653116 @default.
• W291482005 cites W170647179 @default.
• W291482005 cites W1967295852 @default.
• W291482005 cites W1968839848 @default.
• W291482005 cites W1968852205 @default.
• W291482005 cites W1972116890 @default.
• W291482005 cites W1984587196 @default.
• W291482005 cites W2045854456 @default.
• W291482005 cites W2050937329 @default.
• W291482005 cites W2059288217 @default.
• W291482005 cites W2065480000 @default.
• W291482005 cites W2071733283 @default.
• W291482005 cites W2075847325 @default.
• W291482005 cites W2086763790 @default.
• W291482005 cites W2087428164 @default.
• W291482005 cites W2111275724 @default.
• W291482005 cites W2121245482 @default.
• W291482005 cites W2129722343 @default.
• W291482005 cites W2142809896 @default.
• W291482005 cites W2166876123 @default.
• W291482005 cites W2416317892 @default.
• W291482005 cites W2416946457 @default.
• W291482005 cites W2434456953 @default.
• W291482005 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1384030" @default.
• W291482005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7635527" @default.
• W291482005 hasPublicationYear "1995" @default.
• W291482005 type Work @default.
• W291482005 sameAs 291482005 @default.
• W291482005 citedByCount "10" @default.
• W291482005 crossrefType "journal-article" @default.
• W291482005 hasAuthorship W291482005A5009160829 @default.
• W291482005 hasAuthorship W291482005A5009892820 @default.
• W291482005 hasAuthorship W291482005A5013200935 @default.
• W291482005 hasAuthorship W291482005A5016818733 @default.
• W291482005 hasAuthorship W291482005A5029878487 @default.
• W291482005 hasAuthorship W291482005A5058147541 @default.
• W291482005 hasAuthorship W291482005A5064145324 @default.
• W291482005 hasAuthorship W291482005A5067085999 @default.
• W291482005 hasAuthorship W291482005A5067870640 @default.
• W291482005 hasAuthorship W291482005A5087627289 @default.
• W291482005 hasConcept C104317684 @default.
• W291482005 hasConcept C147483822 @default.
• W291482005 hasConcept C153911025 @default.
• W291482005 hasConcept C159047783 @default.
• W291482005 hasConcept C195616568 @default.
• W291482005 hasConcept C202751555 @default.
• W291482005 hasConcept C203014093 @default.
• W291482005 hasConcept C2522874641 @default.
• W291482005 hasConcept C2776258796 @default.
• W291482005 hasConcept C40479148 @default.
• W291482005 hasConcept C49105822 @default.
• W291482005 hasConcept C54355233 @default.
• W291482005 hasConcept C55493867 @default.
• W291482005 hasConcept C81885089 @default.
• W291482005 hasConcept C86803240 @default.
• W291482005 hasConceptScore W291482005C104317684 @default.
• W291482005 hasConceptScore W291482005C147483822 @default.
• W291482005 hasConceptScore W291482005C153911025 @default.
• W291482005 hasConceptScore W291482005C159047783 @default.
• W291482005 hasConceptScore W291482005C195616568 @default.
• W291482005 hasConceptScore W291482005C202751555 @default.
• W291482005 hasConceptScore W291482005C203014093 @default.
• W291482005 hasConceptScore W291482005C2522874641 @default.
• W291482005 hasConceptScore W291482005C2776258796 @default.
• W291482005 hasConceptScore W291482005C40479148 @default.
• W291482005 hasConceptScore W291482005C49105822 @default.
• W291482005 hasConceptScore W291482005C54355233 @default.
• W291482005 hasConceptScore W291482005C55493867 @default.
• W291482005 hasConceptScore W291482005C81885089 @default.
• W291482005 hasConceptScore W291482005C86803240 @default.
• W291482005 hasIssue "1" @default.
• W291482005 hasLocation W2914820051 @default.
• W291482005 hasOpenAccess W291482005 @default.
• W291482005 hasPrimaryLocation W2914820051 @default.
• W291482005 hasRelatedWork W1487857142 @default.
• W291482005 hasRelatedWork W1966559400 @default.
• W291482005 hasRelatedWork W1992360229 @default.
• W291482005 hasRelatedWork W2032459272 @default.

• next