Matches in SemOpenAlex for { <https://semopenalex.org/work/W2914973094> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2914973094 endingPage "16" @default.
- W2914973094 startingPage "16" @default.
- W2914973094 abstract "Unnatural proteins are crucial biomacromolecules and have been widely applied in fundamental science, novel biopolymer materials, enzymes, and therapeutics. Cell-free protein synthesis (CFPS) system can serve as a robust platform to synthesize unnatural proteins by highly effective site-specific incorporation of unnatural amino acids (UNAAs), without the limitations of cell membrane permeability and the toxicity of unnatural components. Here, we describe a quick and simple method to synthesize unnatural proteins in CFPS system based on Escherichia coli crude extract, with unnatural orthogonal aminoacyl-tRNA synthetase and suppressor tRNA evolved from Methanocaldococcus jannaschii. The superfolder green fluorescent protein (sfGFP) and p-propargyloxyphenylalanine (pPaF) were used as the model protein and UNAA. The synthesis of unnatural sfGFPs was characterized by microplate spectrophotometer, affinity chromatography, and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). This protocol provides a detailed procedure guiding how to use the powerful CFPS system to synthesize unnatural proteins on demand." @default.
- W2914973094 created "2019-02-21" @default.
- W2914973094 creator A5070472934 @default.
- W2914973094 creator A5079273603 @default.
- W2914973094 creator A5085049693 @default.
- W2914973094 date "2019-02-12" @default.
- W2914973094 modified "2023-10-16" @default.
- W2914973094 title "Efficient Incorporation of Unnatural Amino Acids into Proteins with a Robust Cell-Free System" @default.
- W2914973094 cites W1970743827 @default.
- W2914973094 cites W1978880815 @default.
- W2914973094 cites W2012114611 @default.
- W2914973094 cites W2017765651 @default.
- W2914973094 cites W2039552715 @default.
- W2914973094 cites W2051556659 @default.
- W2914973094 cites W2052060414 @default.
- W2914973094 cites W2052061962 @default.
- W2914973094 cites W2077116582 @default.
- W2914973094 cites W2083369386 @default.
- W2914973094 cites W2102755926 @default.
- W2914973094 cites W2114795070 @default.
- W2914973094 cites W2118568444 @default.
- W2914973094 cites W2211918045 @default.
- W2914973094 cites W2266253078 @default.
- W2914973094 cites W2313602511 @default.
- W2914973094 cites W2529250862 @default.
- W2914973094 cites W2562682295 @default.
- W2914973094 cites W2593517473 @default.
- W2914973094 cites W914903399 @default.
- W2914973094 doi "https://doi.org/10.3390/mps2010016" @default.
- W2914973094 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6481062" @default.
- W2914973094 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31164598" @default.
- W2914973094 hasPublicationYear "2019" @default.
- W2914973094 type Work @default.
- W2914973094 sameAs 2914973094 @default.
- W2914973094 citedByCount "21" @default.
- W2914973094 countsByYear W29149730942019 @default.
- W2914973094 countsByYear W29149730942020 @default.
- W2914973094 countsByYear W29149730942021 @default.
- W2914973094 countsByYear W29149730942022 @default.
- W2914973094 countsByYear W29149730942023 @default.
- W2914973094 crossrefType "journal-article" @default.
- W2914973094 hasAuthorship W2914973094A5070472934 @default.
- W2914973094 hasAuthorship W2914973094A5079273603 @default.
- W2914973094 hasAuthorship W2914973094A5085049693 @default.
- W2914973094 hasBestOaLocation W29149730941 @default.
- W2914973094 hasConcept C104317684 @default.
- W2914973094 hasConcept C153957851 @default.
- W2914973094 hasConcept C185592680 @default.
- W2914973094 hasConcept C21951064 @default.
- W2914973094 hasConcept C2778665413 @default.
- W2914973094 hasConcept C3675279 @default.
- W2914973094 hasConcept C515207424 @default.
- W2914973094 hasConcept C547475151 @default.
- W2914973094 hasConcept C55493867 @default.
- W2914973094 hasConcept C67705224 @default.
- W2914973094 hasConceptScore W2914973094C104317684 @default.
- W2914973094 hasConceptScore W2914973094C153957851 @default.
- W2914973094 hasConceptScore W2914973094C185592680 @default.
- W2914973094 hasConceptScore W2914973094C21951064 @default.
- W2914973094 hasConceptScore W2914973094C2778665413 @default.
- W2914973094 hasConceptScore W2914973094C3675279 @default.
- W2914973094 hasConceptScore W2914973094C515207424 @default.
- W2914973094 hasConceptScore W2914973094C547475151 @default.
- W2914973094 hasConceptScore W2914973094C55493867 @default.
- W2914973094 hasConceptScore W2914973094C67705224 @default.
- W2914973094 hasFunder F4320321001 @default.
- W2914973094 hasIssue "1" @default.
- W2914973094 hasLocation W29149730941 @default.
- W2914973094 hasLocation W29149730942 @default.
- W2914973094 hasLocation W29149730943 @default.
- W2914973094 hasLocation W29149730944 @default.
- W2914973094 hasOpenAccess W2914973094 @default.
- W2914973094 hasPrimaryLocation W29149730941 @default.
- W2914973094 hasRelatedWork W1971333808 @default.
- W2914973094 hasRelatedWork W2031322846 @default.
- W2914973094 hasRelatedWork W2049462770 @default.
- W2914973094 hasRelatedWork W2086416196 @default.
- W2914973094 hasRelatedWork W2108318551 @default.
- W2914973094 hasRelatedWork W2115994761 @default.
- W2914973094 hasRelatedWork W2120744038 @default.
- W2914973094 hasRelatedWork W2345257221 @default.
- W2914973094 hasRelatedWork W2731175349 @default.
- W2914973094 hasRelatedWork W2913072500 @default.
- W2914973094 hasVolume "2" @default.
- W2914973094 isParatext "false" @default.
- W2914973094 isRetracted "false" @default.
- W2914973094 magId "2914973094" @default.
- W2914973094 workType "article" @default.