Matches in SemOpenAlex for { <https://semopenalex.org/work/W2915032512> ?p ?o ?g. }
- W2915032512 endingPage "e0211393" @default.
- W2915032512 startingPage "e0211393" @default.
- W2915032512 abstract "Cryptococcosis is an Invasive Fungal Infection (IFI) caused by Cryptococcus neoformans, mainly in immunocompromised patients. Therapeutic failure due to pathogen drug resistance, treatment inconstancy and few antifungal options is a problem. The study of amino acid biosynthesis and uptake represents an opportunity to explore possible development of novel antifungals. C. neoformans has 10 amino acids permeases, two of them (Aap3 and Aap7) not expressed at the conditions tested, and five were studied previously (Aap2, Aap4, Aap5, Mup1 and Mup3). Our previous results showed that Aap4 and Aap5 are major permeases with overlapping functions. The aap4Δ/aap5Δ double mutant fails to grow in amino acids as sole nitrogen source and is avirulent in animal model. Here, we deleted the remaining amino acid permeases (AAP1, AAP6, AAP8) that showed gene expression modulation by nutritional condition and created a double mutant (aap1Δ/aap2Δ). We studied the virulence attributes of these mutants and explored the regulatory mechanism behind amino acid uptake in C. neoformans. The aap1Δ/aap2Δ strain had reduced growth at 37°C in L-amino acids, reduced capsule production and was hypovirulent in the Galleria mellonella animal model. Our data, along with previous studies, (i) complement the analysis for all 10 amino acid permeases mutants, (ii) corroborate the idea that these transporters behave as global permeases, (iii) are required during heat and nutritional stress, and (iv) are important for virulence. Our study also indicates a new possible link between Ras1 signaling and amino acids uptake." @default.
- W2915032512 created "2019-02-21" @default.
- W2915032512 creator A5025949070 @default.
- W2915032512 creator A5029490674 @default.
- W2915032512 creator A5045241205 @default.
- W2915032512 creator A5065792899 @default.
- W2915032512 creator A5068188287 @default.
- W2915032512 creator A5068245119 @default.
- W2915032512 creator A5084178812 @default.
- W2915032512 creator A5091133047 @default.
- W2915032512 date "2019-01-25" @default.
- W2915032512 modified "2023-10-01" @default.
- W2915032512 title "Amino acid permeases in Cryptococcus neoformans are required for high temperature growth and virulence; and are regulated by Ras signaling" @default.
- W2915032512 cites W1219431034 @default.
- W2915032512 cites W138087915 @default.
- W2915032512 cites W1535070510 @default.
- W2915032512 cites W1872803163 @default.
- W2915032512 cites W1925046267 @default.
- W2915032512 cites W1944361623 @default.
- W2915032512 cites W1963311521 @default.
- W2915032512 cites W1971170967 @default.
- W2915032512 cites W1981239770 @default.
- W2915032512 cites W1994648408 @default.
- W2915032512 cites W1995941287 @default.
- W2915032512 cites W1996783999 @default.
- W2915032512 cites W2002155770 @default.
- W2915032512 cites W2003079967 @default.
- W2915032512 cites W2003092273 @default.
- W2915032512 cites W2003154726 @default.
- W2915032512 cites W2005800004 @default.
- W2915032512 cites W2007893118 @default.
- W2915032512 cites W2017811519 @default.
- W2915032512 cites W2024812116 @default.
- W2915032512 cites W2027209297 @default.
- W2915032512 cites W2028025575 @default.
- W2915032512 cites W2029192414 @default.
- W2915032512 cites W2033897927 @default.
- W2915032512 cites W2035502041 @default.
- W2915032512 cites W2045776897 @default.
- W2915032512 cites W2049491272 @default.
- W2915032512 cites W2052789908 @default.
- W2915032512 cites W2058333934 @default.
- W2915032512 cites W2062709789 @default.
- W2915032512 cites W2063779731 @default.
- W2915032512 cites W2071779987 @default.
- W2915032512 cites W2072693339 @default.
- W2915032512 cites W2084574037 @default.
- W2915032512 cites W2089269684 @default.
- W2915032512 cites W2094041961 @default.
- W2915032512 cites W2103485717 @default.
- W2915032512 cites W2107277218 @default.
- W2915032512 cites W2111030334 @default.
- W2915032512 cites W2114409750 @default.
- W2915032512 cites W2115175660 @default.
- W2915032512 cites W2119880324 @default.
- W2915032512 cites W2120277859 @default.
- W2915032512 cites W2121749642 @default.
- W2915032512 cites W2122002065 @default.
- W2915032512 cites W2122798106 @default.
- W2915032512 cites W2126519916 @default.
- W2915032512 cites W2127363691 @default.
- W2915032512 cites W2131386377 @default.
- W2915032512 cites W2133909443 @default.
- W2915032512 cites W2134624875 @default.
- W2915032512 cites W2135795082 @default.
- W2915032512 cites W2136579557 @default.
- W2915032512 cites W2140957436 @default.
- W2915032512 cites W2144353353 @default.
- W2915032512 cites W2152193146 @default.
- W2915032512 cites W2152955612 @default.
- W2915032512 cites W2154555878 @default.
- W2915032512 cites W2159227723 @default.
- W2915032512 cites W2161188281 @default.
- W2915032512 cites W2161316221 @default.
- W2915032512 cites W2166939812 @default.
- W2915032512 cites W2170973477 @default.
- W2915032512 cites W2192080449 @default.
- W2915032512 cites W2407688173 @default.
- W2915032512 cites W243079712 @default.
- W2915032512 cites W2474991451 @default.
- W2915032512 cites W2497375665 @default.
- W2915032512 cites W2525772736 @default.
- W2915032512 cites W2528943852 @default.
- W2915032512 cites W2564777824 @default.
- W2915032512 cites W2586809048 @default.
- W2915032512 cites W2606618714 @default.
- W2915032512 cites W2611697423 @default.
- W2915032512 cites W2780539894 @default.
- W2915032512 cites W2781848668 @default.
- W2915032512 cites W2792135755 @default.
- W2915032512 cites W2800919333 @default.
- W2915032512 cites W2810959900 @default.
- W2915032512 cites W2883592189 @default.
- W2915032512 cites W4211092440 @default.
- W2915032512 cites W4232192817 @default.
- W2915032512 cites W4234048494 @default.
- W2915032512 cites W2558327348 @default.
- W2915032512 doi "https://doi.org/10.1371/journal.pone.0211393" @default.