FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2915297581> ?p ?o ?g. }

• W2915297581 endingPage "e0007191" @default.
• W2915297581 startingPage "e0007191" @default.
• W2915297581 abstract "Background Robust protocols for the isolation of extracellular vesicles (EVs) from the rest of their excretory-secretory products are necessary for downstream studies and application development. The most widely used purification method of EVs for helminth pathogens is currently differential centrifugation (DC). In contrast, size exclusion chromatography (SEC) has been included in the purification pipeline for EVs from other pathogens, highlighting there is not an agreed research community ‘gold standard’ for EV isolation. In this case study, Fasciola hepatica from natural populations were cultured in order to collect EVs from culture media and evaluate a SEC or DC approach to pathogen helminth EV purification. Methodology/Principal findings Transmission electron and atomic force microscopy demonstrated that EVs prepared by SEC were both smaller in size and less diverse than EV resolved by DC. Protein quantification and Western blotting further demonstrated that SEC purification realised a higher EV purity to free excretory-secretory protein (ESP) yield ratio compared to DC approaches as evident by the reduction of soluble free cathepsin L proteases in SEC EV preparations. Proteomic analysis further highlighted DC contamination from ESP as shown by an increased diversity of protein identifications and unique peptide hits in DC EVs as compared to SEC EVs. In addition, SEC purified EVs contained less tegumental based proteins than DC purified EVs. Conclusions/Significance The data suggests that DC and SEC purification methods do not isolate equivalent EV population profiles and caution should be taken in the choice of EV purification utilised, with certain protocols for DC preparations including more free ES proteins and tegumental artefacts. We propose that SEC methods should be used for EV purification prior to downstream studies." @default.
• W2915297581 created "2019-03-02" @default.
• W2915297581 creator A5000794908 @default.
• W2915297581 creator A5004215500 @default.
• W2915297581 creator A5036354366 @default.
• W2915297581 creator A5043207237 @default.
• W2915297581 creator A5053816118 @default.
• W2915297581 creator A5065809831 @default.
• W2915297581 creator A5081427867 @default.
• W2915297581 date "2019-02-27" @default.
• W2915297581 modified "2023-10-16" @default.
• W2915297581 title "The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens" @default.
• W2915297581 cites W1535707905 @default.
• W2915297581 cites W1800636431 @default.
• W2915297581 cites W1839188703 @default.
• W2915297581 cites W1877686344 @default.
• W2915297581 cites W1888751811 @default.
• W2915297581 cites W1914452730 @default.
• W2915297581 cites W1926241811 @default.
• W2915297581 cites W1956207872 @default.
• W2915297581 cites W1971661217 @default.
• W2915297581 cites W1975319088 @default.
• W2915297581 cites W1981234142 @default.
• W2915297581 cites W1985619179 @default.
• W2915297581 cites W1989090719 @default.
• W2915297581 cites W1991743944 @default.
• W2915297581 cites W2001849319 @default.
• W2915297581 cites W2007499410 @default.
• W2915297581 cites W2010639804 @default.
• W2915297581 cites W2013055407 @default.
• W2915297581 cites W2014681081 @default.
• W2915297581 cites W2016706539 @default.
• W2915297581 cites W2018106032 @default.
• W2915297581 cites W2018122290 @default.
• W2915297581 cites W2018289835 @default.
• W2915297581 cites W2020863352 @default.
• W2915297581 cites W2026039738 @default.
• W2915297581 cites W2038944440 @default.
• W2915297581 cites W2057491043 @default.
• W2915297581 cites W2067120071 @default.
• W2915297581 cites W2076637458 @default.
• W2915297581 cites W2087888043 @default.
• W2915297581 cites W2089220247 @default.
• W2915297581 cites W2092275783 @default.
• W2915297581 cites W2092788370 @default.
• W2915297581 cites W2097761110 @default.
• W2915297581 cites W2101108802 @default.
• W2915297581 cites W2102517059 @default.
• W2915297581 cites W2109794910 @default.
• W2915297581 cites W2115787145 @default.
• W2915297581 cites W2118767511 @default.
• W2915297581 cites W2121875081 @default.
• W2915297581 cites W2125198788 @default.
• W2915297581 cites W2144026504 @default.
• W2915297581 cites W2144818372 @default.
• W2915297581 cites W2150904674 @default.
• W2915297581 cites W2152467862 @default.
• W2915297581 cites W2155271571 @default.
• W2915297581 cites W2164783491 @default.
• W2915297581 cites W2176750505 @default.
• W2915297581 cites W2200617834 @default.
• W2915297581 cites W2232601925 @default.
• W2915297581 cites W2232891548 @default.
• W2915297581 cites W2237583164 @default.
• W2915297581 cites W2259481044 @default.
• W2915297581 cites W2285639334 @default.
• W2915297581 cites W2288512038 @default.
• W2915297581 cites W2300410725 @default.
• W2915297581 cites W2312700816 @default.
• W2915297581 cites W2325369955 @default.
• W2915297581 cites W2345882633 @default.
• W2915297581 cites W2414083225 @default.
• W2915297581 cites W2491017452 @default.
• W2915297581 cites W2507700562 @default.
• W2915297581 cites W2522802829 @default.
• W2915297581 cites W2530854665 @default.
• W2915297581 cites W2540034041 @default.
• W2915297581 cites W2548862806 @default.
• W2915297581 cites W2558742480 @default.
• W2915297581 cites W2569149698 @default.
• W2915297581 cites W2583743622 @default.
• W2915297581 cites W2601301923 @default.
• W2915297581 cites W2614567943 @default.
• W2915297581 cites W2618954565 @default.
• W2915297581 cites W2623047193 @default.
• W2915297581 cites W2624377772 @default.
• W2915297581 cites W2756078572 @default.
• W2915297581 cites W2767654638 @default.
• W2915297581 cites W2769021844 @default.
• W2915297581 cites W2774233090 @default.
• W2915297581 cites W2793625129 @default.
• W2915297581 cites W564823130 @default.
• W2915297581 doi "https://doi.org/10.1371/journal.pntd.0007191" @default.
• W2915297581 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6411213" @default.
• W2915297581 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30811394" @default.
• W2915297581 hasPublicationYear "2019" @default.
• W2915297581 type Work @default.
• W2915297581 sameAs 2915297581 @default.

• next