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- W2915435202 abstract "Single-cell DNA sequencing is a powerful tool to evaluate the state of heterogeneity of heterogeneous tissues like cancer in a quantitative manner that bulk sequencing can never achieve. DOP-PCR (Degenerate Oligonucleotide-Primed Polymerase Chain Reaction), MDA (Multiple Displacement Amplification), MALBAC (Multiple Annealing and Looping-Based Amplification Cycles), LIANTI (Linear Amplification via Transposon Insertion) and TnBC (Transposon Barcoded) have been the primary choices to prepare single-cell libraries. TnBC library prep method is a simple and versatile methodology, to detect copy number variations or to obtain the absolute copy numbers of genes per cell." @default.
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- W2915435202 date "2019-01-01" @default.
- W2915435202 modified "2023-10-03" @default.
- W2915435202 title "Preparing Single-cell DNA Library Using Nextera for Detection of CNV" @default.
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- W2915435202 doi "https://doi.org/10.21769/bioprotoc.3175" @default.
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