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• W2915660748 abstract "Three‐photon microscopy excited at the 1700‐nm window (roughly covering 1600‐1840 nm) is especially suitable for deep‐brain imaging in living animals. To match the brain refractive index, D 2 O has been exclusively used as the immersion medium. However, the hygroscopic property of D 2 O leads to a decrease of transmittance of the excitation light and as a result a decrease in three‐photon signals over time. Solutions such as replacing D 2 O from time to time, wrapping both the objective lens and the immersion D 2 O, and sealing D 2 O with paraffin liquid have all been demonstrated, which add to the system complexity. Based on our recent characterization of immersion oils, we propose using silicone oil as a potential alternative to D 2 O for deep‐brain imaging. Excited at 1600 nm, our comparative deep‐brain imaging using both D 2 O and silicone oil immersion show that silicone oil immersion yields 17% higher three‐photon signal in third‐harmonic generation imaging within the white matter. Besides, silicone oil immersion also enables three‐photon fluorescence imaging of vasculature up to 1460 μm (mechanical depth) into the mouse brain in vivo acquired at 2 seconds/frame. Together with the nonhygroscopic physical property, silicone oil is promising for long‐span three‐photon brain imaging excited at the 1700‐nm window." @default.
• W2915660748 created "2019-03-02" @default.
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• W2915660748 date "2019-03-13" @default.
• W2915660748 modified "2023-09-23" @default.
• W2915660748 title "Deep‐brain three‐photon microscopy excited at 1600 nm with silicone oil immersion" @default.
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• W2915660748 doi "https://doi.org/10.1002/jbio.201800423" @default.
• W2915660748 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30801979" @default.
• W2915660748 hasPublicationYear "2019" @default.
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