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- W2915695958 abstract "This study was designed to develop a immunofluorescence chromatography kit for detection of CA153 in human serum, and to evaluate the method performance. The fluorescent polystyrene particle conjugated with the monoclonal antibody MIC15 was used as a fluorescent label. The monoclonal antibody MIC16 and mouse anti-rabbit IgG were immobilized on the nitrocellulose membrane as the test line and control line. The linear response rang of the method to detect the serum CA153 was 0.36–250U/ml. And the intra-and inter-assay coefficient were 5.81% and 9.88%. Total 1200 samples were tested by the immunofluorescence chromatography kit, and the overall coincidence rate was 96.35%. The positive coincidence rate was 95.62%, the negative coincidence rate was 97.23%, and the Kappa value surpassed 0.8, which indicated that there was no significant difference between the results of the immunofluorescence chromatography kit and of isolated culture. In conclusion, the immunofluorescence chromatography kit for assaying CA153 provided high sensitivity, rapid, simple operation, low cost, single-servings and can be extensively used for clinical operarions." @default.
- W2915695958 created "2019-03-02" @default.
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- W2915695958 date "2017-08-01" @default.
- W2915695958 modified "2023-09-25" @default.
- W2915695958 title "The establishment of detecting carbohydrate antigen 153 (CA153) with immunofluorescence chromatography" @default.
- W2915695958 doi "https://doi.org/10.1109/icarm.2017.8273175" @default.
- W2915695958 hasPublicationYear "2017" @default.
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