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• W2915698316 abstract "We present a rapid, scalable, user-friendly method for in vitro production of high-purity single-stranded DNA (ssDNA) ranging from 89–3315 nucleotides in length. PCR with a forward primer bearing a methanol-responsive polymer generates a tagged amplicon that enables selective precipitation of the modified strand under denaturing conditions. We demonstrate that the recovered ssDNA can be used for CRISPR/Cas9 homology-directed repair in human cells, DNA-origami folding, and fluorescent in situ hybridization." @default.
• W2915698316 created "2019-03-02" @default.
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• W2915698316 date "2019-02-22" @default.
• W2915698316 modified "2023-09-27" @default.
• W2915698316 title "Rapid and scalable in vitro production of single-stranded DNA" @default.
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• W2915698316 doi "https://doi.org/10.1101/558429" @default.
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