FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2915956887> ?p ?o ?g. }

• W2915956887 abstract "Background Interaction between different types of T-cells and surrounding non-hematopoietic cells is essential for the proper function of the immune system. Here, we focus on the synoviocytes from RA patients and Endothelial Cells (EC) and compare their interaction with Th1 and Th17 cells. Methods To assess the interaction of T cells with stromal cells, the effects of Th1 or Th17 cytokines, Th1 or Th17 clone supernatants and coculture of Th clones and stromal cells were analysed. HUVEC (Human Umbilical Vein Endothelial Cells) were used as a model for EC and synoviocytes were isolated from synovium from RA patients. CD4 + T cells, derived from PBMCs of healthy donors, were used to obtain T-cell clones. CD4 + T-cell clones were classified on the basis of their ability to produce IFN-γ and/or IL-17. T cell clones were polyclonally stimulated with anti-CD3/CD28. Cytokine expression was assessed with RT-PCR and production in supernatants by ELISA. Results As previously described, IL-17A and TNF-α had a synergistic effect for both cells. Moreover, a stronger induction for IL-6 pro- duction was seen for synoviocytes compared to EC (1005 ± 22 pg/ml versus 250 ± 15 ng/ml respectively). Supernatants from inactivated Th1 or Th17 clones had no effect on EC. Regarding synoviocytes, supernatants from inactivated clones induced IL-6 and IL-8 mRNA with a stronger effect for Th17 cells (1000 fold versus 100 fold compared to resting synoviocytes, p = 0.045). While activated Th1 supernatants had a strong effect on EC and increased the expression of IL-6, IL-8, E-selectin, and tissue factor mRNA (98.12, 89 fold respectively, compare to control), Th17 supernatants had no effect. Activated Th1 and Th17 supernatants had the same effect on synoviocytes (254 and 754 fold compared to control). Th1 cells were more potent inducers of IL-6, IL-8 and tissue factor mRNA in EC than Th17 which had no significant effect (5, 12.5, 7.5 fold respectively compared to control). Th17 cells were most effective to stimulate IL-6 and IL-8 mRNA expression in synoviocytes compared to Th1 (100 fold versus 24 for IL-6 mRNA, p = 0.027). Anti-IL-17 antibody reduced IL-6 production from 20.0 ± 4.5 ng/mL to 13.2 ± 3.9 ng/ml (p = 0.032) in synoviocytes cultured with Th17 clones. Conclusions While Th1 cells were able to induce inflammatory cytokine expression in EC and synoviocytes, Th17 cells were only active to promote inflammation in inflammatory cells such as synoviocytes. The effect of Th17 cells appears to depend of IL-17 and the type of stromal cells." @default.
• W2915956887 created "2019-03-02" @default.
• W2915956887 creator A5011711495 @default.
• W2915956887 creator A5016322649 @default.
• W2915956887 creator A5021103847 @default.
• W2915956887 creator A5026642894 @default.
• W2915956887 creator A5089310225 @default.
• W2915956887 date "2013-02-25" @default.
• W2915956887 modified "2023-10-01" @default.
• W2915956887 title "A3.7 Comparison of the Effects of Th17 and Th1 Cells on Endothelial Cells and Synoviocytes" @default.
• W2915956887 doi "https://doi.org/10.1136/annrheumdis-2013-203216.7" @default.
• W2915956887 hasPublicationYear "2013" @default.
• W2915956887 type Work @default.
• W2915956887 sameAs 2915956887 @default.
• W2915956887 citedByCount "0" @default.
• W2915956887 crossrefType "journal-article" @default.
• W2915956887 hasAuthorship W2915956887A5011711495 @default.
• W2915956887 hasAuthorship W2915956887A5016322649 @default.
• W2915956887 hasAuthorship W2915956887A5021103847 @default.
• W2915956887 hasAuthorship W2915956887A5026642894 @default.
• W2915956887 hasAuthorship W2915956887A5089310225 @default.
• W2915956887 hasBestOaLocation W29159568871 @default.
• W2915956887 hasConcept C123012128 @default.
• W2915956887 hasConcept C126322002 @default.
• W2915956887 hasConcept C137061746 @default.
• W2915956887 hasConcept C148125776 @default.
• W2915956887 hasConcept C153911025 @default.
• W2915956887 hasConcept C16930146 @default.
• W2915956887 hasConcept C202751555 @default.
• W2915956887 hasConcept C203014093 @default.
• W2915956887 hasConcept C2776090121 @default.
• W2915956887 hasConcept C2777411675 @default.
• W2915956887 hasConcept C2778690821 @default.
• W2915956887 hasConcept C54355233 @default.
• W2915956887 hasConcept C55493867 @default.
• W2915956887 hasConcept C71924100 @default.
• W2915956887 hasConcept C81885089 @default.
• W2915956887 hasConcept C86803240 @default.
• W2915956887 hasConcept C8891405 @default.
• W2915956887 hasConceptScore W2915956887C123012128 @default.
• W2915956887 hasConceptScore W2915956887C126322002 @default.
• W2915956887 hasConceptScore W2915956887C137061746 @default.
• W2915956887 hasConceptScore W2915956887C148125776 @default.
• W2915956887 hasConceptScore W2915956887C153911025 @default.
• W2915956887 hasConceptScore W2915956887C16930146 @default.
• W2915956887 hasConceptScore W2915956887C202751555 @default.
• W2915956887 hasConceptScore W2915956887C203014093 @default.
• W2915956887 hasConceptScore W2915956887C2776090121 @default.
• W2915956887 hasConceptScore W2915956887C2777411675 @default.
• W2915956887 hasConceptScore W2915956887C2778690821 @default.
• W2915956887 hasConceptScore W2915956887C54355233 @default.
• W2915956887 hasConceptScore W2915956887C55493867 @default.
• W2915956887 hasConceptScore W2915956887C71924100 @default.
• W2915956887 hasConceptScore W2915956887C81885089 @default.
• W2915956887 hasConceptScore W2915956887C86803240 @default.
• W2915956887 hasConceptScore W2915956887C8891405 @default.
• W2915956887 hasLocation W29159568871 @default.
• W2915956887 hasOpenAccess W2915956887 @default.
• W2915956887 hasPrimaryLocation W29159568871 @default.
• W2915956887 hasRelatedWork W129256357 @default.
• W2915956887 hasRelatedWork W1501452428 @default.
• W2915956887 hasRelatedWork W1525884872 @default.
• W2915956887 hasRelatedWork W1547393557 @default.
• W2915956887 hasRelatedWork W1601633981 @default.
• W2915956887 hasRelatedWork W193194099 @default.
• W2915956887 hasRelatedWork W2027144987 @default.
• W2915956887 hasRelatedWork W2031798296 @default.
• W2915956887 hasRelatedWork W2057457816 @default.
• W2915956887 hasRelatedWork W2105846642 @default.
• W2915956887 hasRelatedWork W2147211613 @default.
• W2915956887 hasRelatedWork W2161916137 @default.
• W2915956887 hasRelatedWork W2360342361 @default.
• W2915956887 hasRelatedWork W2360508859 @default.
• W2915956887 hasRelatedWork W2380122544 @default.
• W2915956887 hasRelatedWork W2389042292 @default.
• W2915956887 hasRelatedWork W2389279179 @default.
• W2915956887 hasRelatedWork W2392006293 @default.
• W2915956887 hasRelatedWork W2414875410 @default.
• W2915956887 hasRelatedWork W3032075346 @default.
• W2915956887 isParatext "false" @default.
• W2915956887 isRetracted "false" @default.
• W2915956887 magId "2915956887" @default.
• W2915956887 workType "article" @default.