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- W2916387552 endingPage "1257" @default.
- W2916387552 startingPage "1257" @default.
- W2916387552 abstract "Open-top light-sheet microscopy is a technique that can potentially enable rapid ex vivo inspection of large tissue surfaces and volumes. Here, we have optimized an open-top light-sheet (OTLS) microscope and image-processing workflow for the comprehensive examination of surgical margin surfaces, and have also developed a novel fluorescent analog of H&E staining that is robust for staining fresh unfixed tissues. Our tissue-staining method can be achieved within 2.5 minutes followed by OTLS microscopy of lumpectomy surfaces at a rate of up to 1.5 cm2/minute. An image atlas is presented to show that OTLS image quality surpasses that of intraoperative frozen sectioning and can approximate that of gold-standard H&E histology of formalin-fixed paraffin-embedded (FFPE) tissues. Qualitative evidence indicates that these intraoperative methods do not interfere with downstream post-operative H&E histology and immunohistochemistry. These results should facilitate the translation of OTLS microscopy for intraoperative guidance of lumpectomy and other surgical oncology procedures." @default.
- W2916387552 created "2019-03-02" @default.
- W2916387552 creator A5007033351 @default.
- W2916387552 creator A5010034326 @default.
- W2916387552 creator A5014254460 @default.
- W2916387552 creator A5018781073 @default.
- W2916387552 creator A5026060144 @default.
- W2916387552 creator A5028635627 @default.
- W2916387552 creator A5047878380 @default.
- W2916387552 creator A5072229921 @default.
- W2916387552 date "2019-02-19" @default.
- W2916387552 modified "2023-10-14" @default.
- W2916387552 title "Rapid pathology of lumpectomy margins with open-top light-sheet (OTLS) microscopy" @default.
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