Matches in SemOpenAlex for { <https://semopenalex.org/work/W2916774911> ?p ?o ?g. }
- W2916774911 endingPage "66" @default.
- W2916774911 startingPage "51" @default.
- W2916774911 abstract "Glyoxal (GO) and methylglyoxal (MGO) are two important biomarkers in diabetes. Analytical methods for determination of GO and MGO in serum samples are either HPLC with UV-Vis (low sensitivity) or MS/MS (expensive) detection. These disadvantages have hampered the introduction of these biomarkers as a routine analyte for diabetes diagnostics into the clinical laboratory. In this study, we introduce a UHPLC method with fluorescence detection for the measurement of GO and MGO in serum samples by pre-column derivatization at neutral pH with 5, 6-diamino-2,4-dihydroxypyrimidine sulfate (DDP) to form lumazines. The method was validated as per FDA guidelines. Using this method, we have determined GO and MGO in a variety of animal serum samples, and for example, determined the GO and MGO concentration in adult bovine serum to be 852 ± 27 and 192 ± 10 nmol/L, respectively. In human serum, GO and MGO levels in non-diabetic subjects (n = 14) were determined to be 154 ± 88 and 98 ± 27 nmol/L, and in serum samples from subjects with diabetes (n = 14) 244 ± 137 and 190 ± 68 nmol/L, respectively. In addition, interference studies showed that physiological serum components did not lead to an artificial increase in the levels of GO and MGO." @default.
- W2916774911 created "2019-03-02" @default.
- W2916774911 creator A5001547015 @default.
- W2916774911 creator A5010759721 @default.
- W2916774911 creator A5023854592 @default.
- W2916774911 creator A5040031252 @default.
- W2916774911 creator A5050431731 @default.
- W2916774911 creator A5055491886 @default.
- W2916774911 creator A5080965119 @default.
- W2916774911 date "2019-05-01" @default.
- W2916774911 modified "2023-10-18" @default.
- W2916774911 title "Determination of glyoxal and methylglyoxal in serum by UHPLC coupled with fluorescence detection" @default.
- W2916774911 cites W174942032 @default.
- W2916774911 cites W1945454793 @default.
- W2916774911 cites W1945607742 @default.
- W2916774911 cites W1969938978 @default.
- W2916774911 cites W1974113243 @default.
- W2916774911 cites W1976850731 @default.
- W2916774911 cites W1980925734 @default.
- W2916774911 cites W1999493479 @default.
- W2916774911 cites W2000228729 @default.
- W2916774911 cites W2001697674 @default.
- W2916774911 cites W2003817153 @default.
- W2916774911 cites W2007659337 @default.
- W2916774911 cites W2008462000 @default.
- W2916774911 cites W2011065483 @default.
- W2916774911 cites W2022660408 @default.
- W2916774911 cites W2023423234 @default.
- W2916774911 cites W2024703652 @default.
- W2916774911 cites W2027183895 @default.
- W2916774911 cites W2027470518 @default.
- W2916774911 cites W2027555302 @default.
- W2916774911 cites W2038184872 @default.
- W2916774911 cites W2038350512 @default.
- W2916774911 cites W2041822787 @default.
- W2916774911 cites W2044636787 @default.
- W2916774911 cites W2045428941 @default.
- W2916774911 cites W2051783189 @default.
- W2916774911 cites W2058731809 @default.
- W2916774911 cites W2059681268 @default.
- W2916774911 cites W2061653788 @default.
- W2916774911 cites W2074437273 @default.
- W2916774911 cites W2076972400 @default.
- W2916774911 cites W2080294402 @default.
- W2916774911 cites W2082593420 @default.
- W2916774911 cites W2085765714 @default.
- W2916774911 cites W2093321550 @default.
- W2916774911 cites W2096438273 @default.
- W2916774911 cites W2105214321 @default.
- W2916774911 cites W2115467405 @default.
- W2916774911 cites W2120293565 @default.
- W2916774911 cites W2121948932 @default.
- W2916774911 cites W2123135727 @default.
- W2916774911 cites W2133022777 @default.
- W2916774911 cites W2160492902 @default.
- W2916774911 cites W2307933541 @default.
- W2916774911 cites W2400795251 @default.
- W2916774911 cites W2617741929 @default.
- W2916774911 doi "https://doi.org/10.1016/j.ab.2019.02.014" @default.
- W2916774911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30796906" @default.
- W2916774911 hasPublicationYear "2019" @default.
- W2916774911 type Work @default.
- W2916774911 sameAs 2916774911 @default.
- W2916774911 citedByCount "16" @default.
- W2916774911 countsByYear W29167749112019 @default.
- W2916774911 countsByYear W29167749112020 @default.
- W2916774911 countsByYear W29167749112021 @default.
- W2916774911 countsByYear W29167749112022 @default.
- W2916774911 countsByYear W29167749112023 @default.
- W2916774911 crossrefType "journal-article" @default.
- W2916774911 hasAuthorship W2916774911A5001547015 @default.
- W2916774911 hasAuthorship W2916774911A5010759721 @default.
- W2916774911 hasAuthorship W2916774911A5023854592 @default.
- W2916774911 hasAuthorship W2916774911A5040031252 @default.
- W2916774911 hasAuthorship W2916774911A5050431731 @default.
- W2916774911 hasAuthorship W2916774911A5055491886 @default.
- W2916774911 hasAuthorship W2916774911A5080965119 @default.
- W2916774911 hasConcept C10390740 @default.
- W2916774911 hasConcept C121332964 @default.
- W2916774911 hasConcept C126322002 @default.
- W2916774911 hasConcept C134018914 @default.
- W2916774911 hasConcept C178790620 @default.
- W2916774911 hasConcept C179998833 @default.
- W2916774911 hasConcept C181199279 @default.
- W2916774911 hasConcept C185592680 @default.
- W2916774911 hasConcept C2776923573 @default.
- W2916774911 hasConcept C2777603720 @default.
- W2916774911 hasConcept C2778592357 @default.
- W2916774911 hasConcept C3020081911 @default.
- W2916774911 hasConcept C43617362 @default.
- W2916774911 hasConcept C55493867 @default.
- W2916774911 hasConcept C555293320 @default.
- W2916774911 hasConcept C62520636 @default.
- W2916774911 hasConcept C71924100 @default.
- W2916774911 hasConcept C91881484 @default.
- W2916774911 hasConceptScore W2916774911C10390740 @default.
- W2916774911 hasConceptScore W2916774911C121332964 @default.
- W2916774911 hasConceptScore W2916774911C126322002 @default.