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- W2916879268 abstract "Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS 481 , which is not specific for Bordetella pertussis . Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS 481 -targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS 481 +) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS 1002 and IS 1001 . Results: The results showed that 2.3% of the samples were false-positive for Bordetella pertussis . Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS 481 PCR is limited in Danish patients." @default.
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- W2916879268 date "2019-02-28" @default.
- W2916879268 modified "2023-09-28" @default.
- W2916879268 title "False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients" @default.
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- W2916879268 doi "https://doi.org/10.2174/1874285801913010051" @default.
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