FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2917202476> ?p ?o ?g. }

• W2917202476 endingPage "126" @default.
• W2917202476 startingPage "123" @default.
• W2917202476 abstract "Cannabis sativa L. has been cultivated and used around the globe for its medicinal properties for millennia1. Some cannabinoids, the hallmark constituents of Cannabis, and their analogues have been investigated extensively for their potential medical applications2. Certain cannabinoid formulations have been approved as prescription drugs in several countries for the treatment of a range of human ailments3. However, the study and medicinal use of cannabinoids has been hampered by the legal scheduling of Cannabis, the low in planta abundances of nearly all of the dozens of known cannabinoids4, and their structural complexity, which limits bulk chemical synthesis. Here we report the complete biosynthesis of the major cannabinoids cannabigerolic acid, Δ9-tetrahydrocannabinolic acid, cannabidiolic acid, Δ9-tetrahydrocannabivarinic acid and cannabidivarinic acid in Saccharomyces cerevisiae, from the simple sugar galactose. To accomplish this, we engineered the native mevalonate pathway to provide a high flux of geranyl pyrophosphate and introduced a heterologous, multi-organism-derived hexanoyl-CoA biosynthetic pathway5. We also introduced the Cannabis genes that encode the enzymes involved in the biosynthesis of olivetolic acid6, as well as the gene for a previously undiscovered enzyme with geranylpyrophosphate:olivetolate geranyltransferase activity and the genes for corresponding cannabinoid synthases7,8. Furthermore, we established a biosynthetic approach that harnessed the promiscuity of several pathway genes to produce cannabinoid analogues. Feeding different fatty acids to our engineered strains yielded cannabinoid analogues with modifications in the part of the molecule that is known to alter receptor binding affinity and potency9. We also demonstrated that our biological system could be complemented by simple synthetic chemistry to further expand the accessible chemical space. Our work presents a platform for the production of natural and unnatural cannabinoids that will allow for more rigorous study of these compounds and could be used in the development of treatments for a variety of human health problems." @default.
• W2917202476 created "2019-03-02" @default.
• W2917202476 creator A5000312223 @default.
• W2917202476 creator A5001293753 @default.
• W2917202476 creator A5002506513 @default.
• W2917202476 creator A5005377453 @default.
• W2917202476 creator A5005384855 @default.
• W2917202476 creator A5006284440 @default.
• W2917202476 creator A5010225578 @default.
• W2917202476 creator A5016729665 @default.
• W2917202476 creator A5017900776 @default.
• W2917202476 creator A5019804925 @default.
• W2917202476 creator A5019809213 @default.
• W2917202476 creator A5037892946 @default.
• W2917202476 creator A5043766259 @default.
• W2917202476 creator A5052699089 @default.
• W2917202476 creator A5053308739 @default.
• W2917202476 creator A5055332125 @default.
• W2917202476 creator A5057715969 @default.
• W2917202476 creator A5063223142 @default.
• W2917202476 creator A5070209979 @default.
• W2917202476 creator A5076158937 @default.
• W2917202476 creator A5090006268 @default.
• W2917202476 date "2019-02-27" @default.
• W2917202476 modified "2023-10-17" @default.
• W2917202476 title "Complete biosynthesis of cannabinoids and their unnatural analogues in yeast" @default.
• W2917202476 cites W1962052985 @default.
• W2917202476 cites W1964386724 @default.
• W2917202476 cites W1966360455 @default.
• W2917202476 cites W1977736913 @default.
• W2917202476 cites W2004724869 @default.
• W2917202476 cites W2014994755 @default.
• W2917202476 cites W2023442988 @default.
• W2917202476 cites W2027161101 @default.
• W2917202476 cites W2058997347 @default.
• W2917202476 cites W2069004062 @default.
• W2917202476 cites W2089047063 @default.
• W2917202476 cites W2107523606 @default.
• W2917202476 cites W2119826244 @default.
• W2917202476 cites W2120227293 @default.
• W2917202476 cites W2127438675 @default.
• W2917202476 cites W2129095580 @default.
• W2917202476 cites W2132926880 @default.
• W2917202476 cites W2133174852 @default.
• W2917202476 cites W2136826170 @default.
• W2917202476 cites W2148161442 @default.
• W2917202476 cites W2152770371 @default.
• W2917202476 cites W2161245622 @default.
• W2917202476 cites W2163614601 @default.
• W2917202476 cites W2164213081 @default.
• W2917202476 cites W2277953252 @default.
• W2917202476 cites W2332641124 @default.
• W2917202476 cites W2336400828 @default.
• W2917202476 cites W2342249984 @default.
• W2917202476 cites W2435067507 @default.
• W2917202476 cites W2559716871 @default.
• W2917202476 cites W2729185095 @default.
• W2917202476 cites W966158533 @default.
• W2917202476 doi "https://doi.org/10.1038/s41586-019-0978-9" @default.
• W2917202476 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30814733" @default.
• W2917202476 hasPublicationYear "2019" @default.
• W2917202476 type Work @default.
• W2917202476 sameAs 2917202476 @default.
• W2917202476 citedByCount "420" @default.
• W2917202476 countsByYear W29172024762019 @default.
• W2917202476 countsByYear W29172024762020 @default.
• W2917202476 countsByYear W29172024762021 @default.
• W2917202476 countsByYear W29172024762022 @default.
• W2917202476 countsByYear W29172024762023 @default.
• W2917202476 crossrefType "journal-article" @default.
• W2917202476 hasAuthorship W2917202476A5000312223 @default.
• W2917202476 hasAuthorship W2917202476A5001293753 @default.
• W2917202476 hasAuthorship W2917202476A5002506513 @default.
• W2917202476 hasAuthorship W2917202476A5005377453 @default.
• W2917202476 hasAuthorship W2917202476A5005384855 @default.
• W2917202476 hasAuthorship W2917202476A5006284440 @default.
• W2917202476 hasAuthorship W2917202476A5010225578 @default.
• W2917202476 hasAuthorship W2917202476A5016729665 @default.
• W2917202476 hasAuthorship W2917202476A5017900776 @default.
• W2917202476 hasAuthorship W2917202476A5019804925 @default.
• W2917202476 hasAuthorship W2917202476A5019809213 @default.
• W2917202476 hasAuthorship W2917202476A5037892946 @default.
• W2917202476 hasAuthorship W2917202476A5043766259 @default.
• W2917202476 hasAuthorship W2917202476A5052699089 @default.
• W2917202476 hasAuthorship W2917202476A5053308739 @default.
• W2917202476 hasAuthorship W2917202476A5055332125 @default.
• W2917202476 hasAuthorship W2917202476A5057715969 @default.
• W2917202476 hasAuthorship W2917202476A5063223142 @default.
• W2917202476 hasAuthorship W2917202476A5070209979 @default.
• W2917202476 hasAuthorship W2917202476A5076158937 @default.
• W2917202476 hasAuthorship W2917202476A5090006268 @default.
• W2917202476 hasBestOaLocation W29172024764 @default.
• W2917202476 hasConcept C104317684 @default.
• W2917202476 hasConcept C170493617 @default.
• W2917202476 hasConcept C181199279 @default.
• W2917202476 hasConcept C185592680 @default.
• W2917202476 hasConcept C2780871563 @default.
• W2917202476 hasConcept C553450214 @default.

• next