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- W2917222364 abstract "[Objective]The aim was to obtain and analyze Se62 gene from Spodoptera exigua multicapsid nucleopolyhedrovirus(SeMNPV) genomic DNA.[Method]The sequence of Se62 gene was obtained by PCR method.The PCR product was cloned into pMD18-T vector to get the recombinant plasmid(T-Se62).Then Se62 gene was sequenced and analyzed.[Result]Nucleotide sequence analysis showed that the amplified sequence was 1 128 bp in length,shared 100% identity with the sequence of GenBank(No.NC_002169) of Se62.Predicted protein from nucleotide sequence of Se62(SE62) had two notable transmembrane regions,and contained much PKC-PHOSPHO-SITE,CK2-PHOSPHO-SITE,ASN_GLYCOSYLATION.BLAST protein results showed that SE62 belonged to baculo_P48 protein super family and had high homology with Autographa califorica multicapsid nucleopolyhedrovirus(AcMNPV) P48(Ac_P48)(52%).The function of SE62 in the course of virus production claims attention.[Conclusion]Se62 gene was cloned,which laid a foundation for further research of the gene functions in the course of virus production." @default.
- W2917222364 created "2019-03-02" @default.
- W2917222364 creator A5080196380 @default.
- W2917222364 date "2011-01-01" @default.
- W2917222364 modified "2023-09-23" @default.
- W2917222364 title "Cloning and Sequence Analysis of Spodoptera exigua Multicapsid Nucleopolyhedrovirus Se62 Gene" @default.
- W2917222364 hasPublicationYear "2011" @default.
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