FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2917305439> ?p ?o ?g. }

• W2917305439 abstract "The shortage of food based feedstocks has been one of the stumbling blocks in industrial biomanufacturing. The acetone bioproduction from the traditional acetone–butanol–ethanol fermentation is limited by the non-specificity of products and competitive utilization of food-based substrates. Using genetically modified Escherichia coli to produce acetone as sole product from the cost-effective non-food based substrates showed great potential to overcome these problems. A novel acetone biosynthetic pathway were constructed based on genes from Clostridium acetobutylicum (thlA encoding for thiolase, adc encoding for acetoacetate decarboxylase, ctfAB encoding for coenzyme A transferase) and Escherichia coli MG1655 (atoB encoding acetyl-CoA acetyltransferase, atoDA encoding for acetyl-CoA: acetoacetyl-CoA transferase subunit α and β). Among these constructs, one recombinant MG1655 derivative containing the hybrid pathway consisting of thlA, atoDA, and adc, produced the highest level of acetone from acetate. Reducing the gluconeogenesis pathway had little effect on acetone production, while blocking the TCA cycle by knocking out the icdA gene enhanced the yield of acetone significantly. As a result, acetone concentration increased up to 113.18 mM in 24 h by the resting cell culture coupling with gas-stripping methods. An engineered E. coli strain with optimized hybrid acetone biosynthetic pathway can utilize acetate as substrate efficiently to synthesize acetone without other non-gas byproducts. It provides a potential method for industrial biomanufacturing of acetone by engineered E. coli strains from non-food based substrate." @default.
• W2917305439 created "2019-03-02" @default.
• W2917305439 creator A5009583749 @default.
• W2917305439 creator A5027297044 @default.
• W2917305439 creator A5029479817 @default.
• W2917305439 creator A5064217312 @default.
• W2917305439 creator A5076251153 @default.
• W2917305439 creator A5080276547 @default.
• W2917305439 creator A5088971303 @default.
• W2917305439 date "2019-01-14" @default.
• W2917305439 modified "2023-10-17" @default.
• W2917305439 title "Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate" @default.
• W2917305439 cites W1867800400 @default.
• W2917305439 cites W1882032094 @default.
• W2917305439 cites W1966173822 @default.
• W2917305439 cites W1979184629 @default.
• W2917305439 cites W1990061397 @default.
• W2917305439 cites W2003696779 @default.
• W2917305439 cites W2020138750 @default.
• W2917305439 cites W2022307288 @default.
• W2917305439 cites W2037722742 @default.
• W2917305439 cites W2060438193 @default.
• W2917305439 cites W2062636982 @default.
• W2917305439 cites W2065399928 @default.
• W2917305439 cites W2079485359 @default.
• W2917305439 cites W2108131456 @default.
• W2917305439 cites W2116137883 @default.
• W2917305439 cites W2119908734 @default.
• W2917305439 cites W2150088285 @default.
• W2917305439 cites W2156528225 @default.
• W2917305439 cites W2204347736 @default.
• W2917305439 cites W2294471624 @default.
• W2917305439 cites W2326410332 @default.
• W2917305439 cites W2331970207 @default.
• W2917305439 cites W2339423968 @default.
• W2917305439 cites W2345895335 @default.
• W2917305439 cites W2472131322 @default.
• W2917305439 cites W2490034073 @default.
• W2917305439 cites W2621165553 @default.
• W2917305439 cites W2775236216 @default.
• W2917305439 cites W2781428063 @default.
• W2917305439 cites W2810508147 @default.
• W2917305439 cites W2884307066 @default.
• W2917305439 cites W2888540619 @default.
• W2917305439 doi "https://doi.org/10.1186/s12934-019-1054-8" @default.
• W2917305439 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6330746" @default.
• W2917305439 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30642338" @default.
• W2917305439 hasPublicationYear "2019" @default.
• W2917305439 type Work @default.
• W2917305439 sameAs 2917305439 @default.
• W2917305439 citedByCount "21" @default.
• W2917305439 countsByYear W29173054392020 @default.
• W2917305439 countsByYear W29173054392021 @default.
• W2917305439 countsByYear W29173054392022 @default.
• W2917305439 countsByYear W29173054392023 @default.
• W2917305439 crossrefType "journal-article" @default.
• W2917305439 hasAuthorship W2917305439A5009583749 @default.
• W2917305439 hasAuthorship W2917305439A5027297044 @default.
• W2917305439 hasAuthorship W2917305439A5029479817 @default.
• W2917305439 hasAuthorship W2917305439A5064217312 @default.
• W2917305439 hasAuthorship W2917305439A5076251153 @default.
• W2917305439 hasAuthorship W2917305439A5080276547 @default.
• W2917305439 hasAuthorship W2917305439A5088971303 @default.
• W2917305439 hasBestOaLocation W29173054391 @default.
• W2917305439 hasConcept C100544194 @default.
• W2917305439 hasConcept C104317684 @default.
• W2917305439 hasConcept C127078168 @default.
• W2917305439 hasConcept C181199279 @default.
• W2917305439 hasConcept C185592680 @default.
• W2917305439 hasConcept C2776928978 @default.
• W2917305439 hasConcept C2777713963 @default.
• W2917305439 hasConcept C2779227428 @default.
• W2917305439 hasConcept C2780161600 @default.
• W2917305439 hasConcept C2781031557 @default.
• W2917305439 hasConcept C2781213060 @default.
• W2917305439 hasConcept C547475151 @default.
• W2917305439 hasConcept C553450214 @default.
• W2917305439 hasConcept C55493867 @default.
• W2917305439 hasConcept C6350086 @default.
• W2917305439 hasConcept C86803240 @default.
• W2917305439 hasConceptScore W2917305439C100544194 @default.
• W2917305439 hasConceptScore W2917305439C104317684 @default.
• W2917305439 hasConceptScore W2917305439C127078168 @default.
• W2917305439 hasConceptScore W2917305439C181199279 @default.
• W2917305439 hasConceptScore W2917305439C185592680 @default.
• W2917305439 hasConceptScore W2917305439C2776928978 @default.
• W2917305439 hasConceptScore W2917305439C2777713963 @default.
• W2917305439 hasConceptScore W2917305439C2779227428 @default.
• W2917305439 hasConceptScore W2917305439C2780161600 @default.
• W2917305439 hasConceptScore W2917305439C2781031557 @default.
• W2917305439 hasConceptScore W2917305439C2781213060 @default.
• W2917305439 hasConceptScore W2917305439C547475151 @default.
• W2917305439 hasConceptScore W2917305439C553450214 @default.
• W2917305439 hasConceptScore W2917305439C55493867 @default.
• W2917305439 hasConceptScore W2917305439C6350086 @default.
• W2917305439 hasConceptScore W2917305439C86803240 @default.
• W2917305439 hasFunder F4320321001 @default.
• W2917305439 hasFunder F4320321885 @default.
• W2917305439 hasFunder F4320334945 @default.
• W2917305439 hasFunder F4320335777 @default.

• next