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- W2917496630 abstract "Niemann–Pick C1 (NPC1) is a membrane protein required for the transport of low-density lipoprotein (LDL)-derived cholesterol from endosomes and lysosomes to the other organelles. Here, we describe the recombinant protein expression, purification, and characterization of the human NPC1. The protein is transiently expressed in human embryonic kidney (HEK) cells. Our purification protocol describes the steps to obtain a pure and homogeneous NPC1 protein. Niemann–Pick C2 (NPC2) is a small soluble protein, which mediates cholesterol transport in tandem with NPC1. Finally, we also describe two biochemical approaches to characterize NPC1 function in vitro—a cholesterol transfer assay from purified NPC2 to NPC1 and a binding assay between NPC1 and NPC2." @default.
- W2917496630 created "2019-03-02" @default.
- W2917496630 creator A5029546833 @default.
- W2917496630 creator A5061459684 @default.
- W2917496630 date "2019-01-01" @default.
- W2917496630 modified "2023-10-18" @default.
- W2917496630 title "Purification and Characterization of Human Niemann–Pick C1 Protein" @default.
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- W2917496630 doi "https://doi.org/10.1007/978-1-4939-9136-5_18" @default.
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- W2917496630 hasPublicationYear "2019" @default.
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