Matches in SemOpenAlex for { <https://semopenalex.org/work/W2918014462> ?p ?o ?g. }
- W2918014462 endingPage "667" @default.
- W2918014462 startingPage "658" @default.
- W2918014462 abstract "We systematically analyzed the synergistic effect of: (i) cytokine-mediated inflammatory activation of endothelial cells (ECs) with and (ii) shear-mediated platelet activation (SMPA) as a potential contributory mechanism to intraventricular thrombus formation in the setting of left ventricular assist device (LVAD) support.Intact and shear-activated human platelets were exposed to non-activated and cytokine-activated ECs. To modulate the level of LVAD-related shear activation, platelets were exposed to shear stress patterns of varying magnitude (30, 50, and 70 dynes/cm2, 10 minutes) via a hemodynamic shearing device. ECs were activated via exposure to inflammatory tumor necrosis factor-α (TNF-α 10 and 100 ng/ml, 24 hours), consistent with inflammatory activation recorded in patients on LVAD circulatory support.Adhesivity of shear-activated platelets to ECs was significantly higher than that of intact/unactivated platelets, regardless of the initial activation level (70 dynes/cm2 shear-activated platelets vs intact platelets: +80%, p < 0.001). Importantly, inflammatory activation of ECs amplified platelet prothrombinase activity progressively with increasing shear stress magnitude and TNF-α concentration: thrombin generation of 70 dynes/cm2 shear-activated platelets was 2.6-fold higher after exposure and adhesion to 100 ng/ml TNF-α‒activated ECs (p < 0.0001).We demonstrated synergistic effect of SMPA and cytokine-mediated EC inflammatory activation to enhance EC‒platelet adhesion and platelet prothrombotic function. These mechanisms may contribute to intraventricular thrombosis in the setting of mechanical circulatory support." @default.
- W2918014462 created "2019-03-02" @default.
- W2918014462 creator A5002274002 @default.
- W2918014462 creator A5012683951 @default.
- W2918014462 creator A5019645693 @default.
- W2918014462 creator A5031595683 @default.
- W2918014462 creator A5036701219 @default.
- W2918014462 creator A5044743374 @default.
- W2918014462 creator A5047132049 @default.
- W2918014462 creator A5051249259 @default.
- W2918014462 creator A5051492953 @default.
- W2918014462 creator A5060255192 @default.
- W2918014462 creator A5064413183 @default.
- W2918014462 creator A5070730048 @default.
- W2918014462 creator A5077168507 @default.
- W2918014462 creator A5079570118 @default.
- W2918014462 creator A5081695706 @default.
- W2918014462 date "2019-06-01" @default.
- W2918014462 modified "2023-10-18" @default.
- W2918014462 title "Prothrombotic activity of cytokine-activated endothelial cells and shear-activated platelets in the setting of ventricular assist device support" @default.
- W2918014462 cites W1490054092 @default.
- W2918014462 cites W1529634963 @default.
- W2918014462 cites W1909159002 @default.
- W2918014462 cites W1970959706 @default.
- W2918014462 cites W1972029688 @default.
- W2918014462 cites W2024003187 @default.
- W2918014462 cites W2032356038 @default.
- W2918014462 cites W2036553170 @default.
- W2918014462 cites W2051014008 @default.
- W2918014462 cites W2060021951 @default.
- W2918014462 cites W2073247033 @default.
- W2918014462 cites W2077156149 @default.
- W2918014462 cites W2082897182 @default.
- W2918014462 cites W2084621368 @default.
- W2918014462 cites W2086095109 @default.
- W2918014462 cites W2087580761 @default.
- W2918014462 cites W2091515332 @default.
- W2918014462 cites W2094032463 @default.
- W2918014462 cites W2103149583 @default.
- W2918014462 cites W2105054347 @default.
- W2918014462 cites W2112504623 @default.
- W2918014462 cites W2113737137 @default.
- W2918014462 cites W2114190018 @default.
- W2918014462 cites W2126844680 @default.
- W2918014462 cites W2129447163 @default.
- W2918014462 cites W2130349041 @default.
- W2918014462 cites W2134766290 @default.
- W2918014462 cites W2137719165 @default.
- W2918014462 cites W2140423139 @default.
- W2918014462 cites W2156117572 @default.
- W2918014462 cites W2157716190 @default.
- W2918014462 cites W2173050817 @default.
- W2918014462 cites W2261175747 @default.
- W2918014462 cites W2261797381 @default.
- W2918014462 cites W2296498874 @default.
- W2918014462 cites W2325468228 @default.
- W2918014462 cites W2347172325 @default.
- W2918014462 cites W2461121939 @default.
- W2918014462 cites W2501166177 @default.
- W2918014462 cites W2530312528 @default.
- W2918014462 cites W2553350361 @default.
- W2918014462 cites W2559940191 @default.
- W2918014462 cites W2567017682 @default.
- W2918014462 cites W2604244160 @default.
- W2918014462 cites W2613972821 @default.
- W2918014462 cites W2726254719 @default.
- W2918014462 cites W2750612035 @default.
- W2918014462 cites W2769047652 @default.
- W2918014462 cites W2772263333 @default.
- W2918014462 cites W2773788026 @default.
- W2918014462 cites W2776973297 @default.
- W2918014462 cites W2777734922 @default.
- W2918014462 cites W2783176782 @default.
- W2918014462 cites W2802764288 @default.
- W2918014462 cites W2810765134 @default.
- W2918014462 cites W2898177938 @default.
- W2918014462 cites W789684726 @default.
- W2918014462 cites W1812676615 @default.
- W2918014462 doi "https://doi.org/10.1016/j.healun.2019.02.009" @default.
- W2918014462 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6535357" @default.
- W2918014462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30846234" @default.
- W2918014462 hasPublicationYear "2019" @default.
- W2918014462 type Work @default.
- W2918014462 sameAs 2918014462 @default.
- W2918014462 citedByCount "15" @default.
- W2918014462 countsByYear W29180144622020 @default.
- W2918014462 countsByYear W29180144622021 @default.
- W2918014462 countsByYear W29180144622022 @default.
- W2918014462 countsByYear W29180144622023 @default.
- W2918014462 crossrefType "journal-article" @default.
- W2918014462 hasAuthorship W2918014462A5002274002 @default.
- W2918014462 hasAuthorship W2918014462A5012683951 @default.
- W2918014462 hasAuthorship W2918014462A5019645693 @default.
- W2918014462 hasAuthorship W2918014462A5031595683 @default.
- W2918014462 hasAuthorship W2918014462A5036701219 @default.
- W2918014462 hasAuthorship W2918014462A5044743374 @default.
- W2918014462 hasAuthorship W2918014462A5047132049 @default.
- W2918014462 hasAuthorship W2918014462A5051249259 @default.