Matches in SemOpenAlex for { <https://semopenalex.org/work/W2918014795> ?p ?o ?g. }
- W2918014795 endingPage "97" @default.
- W2918014795 startingPage "77" @default.
- W2918014795 abstract "The corneal epithelium in humans is the only layer in the cornea that is able to regenerate in vivo, and corneal integrity and function are dependent on this. The ability of the epithelium to regenerate relies on limbal stem cells (LSCs). The LSC niche has been demonstrated to be found within the palisades of Vogt at the corneoscleral limbus. Total limbal stem cell deficiency (LSCD) results in conjunctivalisation of the corneal surface and subsequent permanent visual impairment, together with recurrent or persistent epithelial erosions and ulcerations that lead to stromal opacity and chronic pain and discomfort. In addition to conservative management with lubricating eye drops, serum eye drops, topical steroid and antibiotics, the definitive treatment of total LSCD involves replacing the LSC population before patients can undergo corneal transplantation for visual rehabilitation. This chapter describes the Newcastle methods of autologous limbal stem cell transplantation from the harvesting and culture of autologous LSCs to transplantation of the medicinal product, using a cell suspension method or ex vivo expansion method, describes the Good Manufacturing Practice (GMP) processes for the production of cells for therapeutic use and looks to the future use of a European-licensed LSC therapeutic product (Holoclar®, Chiesi Farmaceutici S.p.A., Parma, Italy) for the treatment of unilateral severe or total LSCD due to ocular surface burns. This chapter also describes the methods for the assessment of the LSC culture, including histological methods, and the potential use of decellularised, γ-irradiated human amniotic membrane as a suitable substrate for LSC culture." @default.
- W2918014795 created "2019-03-02" @default.
- W2918014795 creator A5012252844 @default.
- W2918014795 creator A5032539966 @default.
- W2918014795 creator A5068464460 @default.
- W2918014795 creator A5081989385 @default.
- W2918014795 date "2019-01-01" @default.
- W2918014795 modified "2023-09-28" @default.
- W2918014795 title "Corneal Epithelial Stem Cells: Methods for Ex Vivo Expansion" @default.
- W2918014795 cites W1514351476 @default.
- W2918014795 cites W1554831199 @default.
- W2918014795 cites W1595887419 @default.
- W2918014795 cites W1963604144 @default.
- W2918014795 cites W1966632162 @default.
- W2918014795 cites W1967354060 @default.
- W2918014795 cites W1967479683 @default.
- W2918014795 cites W1968882420 @default.
- W2918014795 cites W1969097782 @default.
- W2918014795 cites W1969100829 @default.
- W2918014795 cites W1972552247 @default.
- W2918014795 cites W1974046502 @default.
- W2918014795 cites W1976170651 @default.
- W2918014795 cites W1978818002 @default.
- W2918014795 cites W1982238146 @default.
- W2918014795 cites W1984133400 @default.
- W2918014795 cites W1987766583 @default.
- W2918014795 cites W1991367509 @default.
- W2918014795 cites W1992744445 @default.
- W2918014795 cites W1993512542 @default.
- W2918014795 cites W1995972022 @default.
- W2918014795 cites W1998791958 @default.
- W2918014795 cites W2000411167 @default.
- W2918014795 cites W2002296022 @default.
- W2918014795 cites W2004790394 @default.
- W2918014795 cites W2004920043 @default.
- W2918014795 cites W2008766968 @default.
- W2918014795 cites W2009846860 @default.
- W2918014795 cites W2011866974 @default.
- W2918014795 cites W2013153771 @default.
- W2918014795 cites W2013442349 @default.
- W2918014795 cites W2013857188 @default.
- W2918014795 cites W2013871655 @default.
- W2918014795 cites W2015927418 @default.
- W2918014795 cites W2018945153 @default.
- W2918014795 cites W2019072415 @default.
- W2918014795 cites W2019886227 @default.
- W2918014795 cites W2020396111 @default.
- W2918014795 cites W2020801013 @default.
- W2918014795 cites W2021966010 @default.
- W2918014795 cites W2023468388 @default.
- W2918014795 cites W2029919789 @default.
- W2918014795 cites W2030826031 @default.
- W2918014795 cites W2031499346 @default.
- W2918014795 cites W2035255316 @default.
- W2918014795 cites W2037092716 @default.
- W2918014795 cites W2037477217 @default.
- W2918014795 cites W2037718179 @default.
- W2918014795 cites W2038723174 @default.
- W2918014795 cites W2042862773 @default.
- W2918014795 cites W2043360705 @default.
- W2918014795 cites W2043634767 @default.
- W2918014795 cites W2051048131 @default.
- W2918014795 cites W2051408850 @default.
- W2918014795 cites W2057518564 @default.
- W2918014795 cites W2059515600 @default.
- W2918014795 cites W2065069982 @default.
- W2918014795 cites W2065251855 @default.
- W2918014795 cites W2070097727 @default.
- W2918014795 cites W2075384925 @default.
- W2918014795 cites W2075864763 @default.
- W2918014795 cites W2076835986 @default.
- W2918014795 cites W2077537705 @default.
- W2918014795 cites W2077597743 @default.
- W2918014795 cites W2078246403 @default.
- W2918014795 cites W2085820087 @default.
- W2918014795 cites W2088579171 @default.
- W2918014795 cites W2089966723 @default.
- W2918014795 cites W2093531961 @default.
- W2918014795 cites W2093583163 @default.
- W2918014795 cites W2094008821 @default.
- W2918014795 cites W2096604029 @default.
- W2918014795 cites W2099154561 @default.
- W2918014795 cites W2099642484 @default.
- W2918014795 cites W2100465544 @default.
- W2918014795 cites W2102245343 @default.
- W2918014795 cites W2115926498 @default.
- W2918014795 cites W2118751485 @default.
- W2918014795 cites W2123900930 @default.
- W2918014795 cites W2126105482 @default.
- W2918014795 cites W2128078747 @default.
- W2918014795 cites W2129640308 @default.
- W2918014795 cites W2132984034 @default.
- W2918014795 cites W2134208433 @default.
- W2918014795 cites W2137411554 @default.
- W2918014795 cites W2142665498 @default.
- W2918014795 cites W2155289759 @default.
- W2918014795 cites W2157730445 @default.
- W2918014795 cites W2167122163 @default.