Matches in SemOpenAlex for { <https://semopenalex.org/work/W2918094865> ?p ?o ?g. }
- W2918094865 endingPage "558" @default.
- W2918094865 startingPage "546" @default.
- W2918094865 abstract "Hybridoma methods for monoclonal antibody (mAb) cloning are a mainstay of biomedical research, but they are hindered by the need to maintain hybridomas in oligoclonal pools during antibody screening. Here, we describe a system in which hybridomas specifically capture and display the mAbs they secrete: On-Cell mAb Screening (OCMS™). In OCMS™, mAbs displayed on the cell surface can be rapidly assayed for expression level and binding specificity using fluorescent antigens with high-content (image-based) methods or flow cytometry. OCMS™ demonstrated specific mAb binding to poliovirus and rabies virus by forming a cell surface IgG “cap”, as a universal assay for anti-viral mAbs. We produced and characterized OCMS™-enabled hybridomas secreting mAbs that neutralize poliovirus and used fluorescence microscopy to identify and clone a human mAb specific for the human N-methyl-D-aspartate receptor. Lastly, we used OCMS™ to assess expression and antigen binding of a recombinant mAb produced in 293T cells. As a novel method to physically associate mAbs with the hybridomas that secrete them, OCMS™ overcomes a central challenge to hybridoma mAb screening and offers new paradigms for mAb discovery and production." @default.
- W2918094865 created "2019-03-02" @default.
- W2918094865 creator A5010542220 @default.
- W2918094865 creator A5023994204 @default.
- W2918094865 creator A5033646110 @default.
- W2918094865 creator A5039038504 @default.
- W2918094865 creator A5048863725 @default.
- W2918094865 creator A5049094904 @default.
- W2918094865 creator A5068823607 @default.
- W2918094865 creator A5075414157 @default.
- W2918094865 creator A5080830950 @default.
- W2918094865 creator A5089381889 @default.
- W2918094865 date "2019-02-22" @default.
- W2918094865 modified "2023-10-16" @default.
- W2918094865 title "Capture and display of antibodies secreted by hybridoma cells enables fluorescent on-cell screening" @default.
- W2918094865 cites W1717642344 @default.
- W2918094865 cites W190722417 @default.
- W2918094865 cites W1951570756 @default.
- W2918094865 cites W1971501807 @default.
- W2918094865 cites W1975801479 @default.
- W2918094865 cites W1976828230 @default.
- W2918094865 cites W1986917490 @default.
- W2918094865 cites W1996101693 @default.
- W2918094865 cites W2011825464 @default.
- W2918094865 cites W2018285317 @default.
- W2918094865 cites W2018836262 @default.
- W2918094865 cites W2023581362 @default.
- W2918094865 cites W2030522563 @default.
- W2918094865 cites W2033042708 @default.
- W2918094865 cites W2043391059 @default.
- W2918094865 cites W2048421209 @default.
- W2918094865 cites W2052853913 @default.
- W2918094865 cites W2054109196 @default.
- W2918094865 cites W2062857909 @default.
- W2918094865 cites W2071333804 @default.
- W2918094865 cites W2072183005 @default.
- W2918094865 cites W2075621143 @default.
- W2918094865 cites W2079277731 @default.
- W2918094865 cites W2080799466 @default.
- W2918094865 cites W2086239639 @default.
- W2918094865 cites W2096387523 @default.
- W2918094865 cites W2108491944 @default.
- W2918094865 cites W2129157800 @default.
- W2918094865 cites W2130479394 @default.
- W2918094865 cites W2146199022 @default.
- W2918094865 cites W2166316275 @default.
- W2918094865 cites W2199567922 @default.
- W2918094865 cites W2273249389 @default.
- W2918094865 cites W2297024106 @default.
- W2918094865 cites W2340102874 @default.
- W2918094865 cites W2413651649 @default.
- W2918094865 cites W2510204423 @default.
- W2918094865 cites W2603365441 @default.
- W2918094865 cites W2761910001 @default.
- W2918094865 cites W2807465248 @default.
- W2918094865 cites W2820181338 @default.
- W2918094865 doi "https://doi.org/10.1080/19420862.2019.1574520" @default.
- W2918094865 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6512912" @default.
- W2918094865 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30794061" @default.
- W2918094865 hasPublicationYear "2019" @default.
- W2918094865 type Work @default.
- W2918094865 sameAs 2918094865 @default.
- W2918094865 citedByCount "11" @default.
- W2918094865 countsByYear W29180948652019 @default.
- W2918094865 countsByYear W29180948652020 @default.
- W2918094865 countsByYear W29180948652021 @default.
- W2918094865 countsByYear W29180948652022 @default.
- W2918094865 countsByYear W29180948652023 @default.
- W2918094865 crossrefType "journal-article" @default.
- W2918094865 hasAuthorship W2918094865A5010542220 @default.
- W2918094865 hasAuthorship W2918094865A5023994204 @default.
- W2918094865 hasAuthorship W2918094865A5033646110 @default.
- W2918094865 hasAuthorship W2918094865A5039038504 @default.
- W2918094865 hasAuthorship W2918094865A5048863725 @default.
- W2918094865 hasAuthorship W2918094865A5049094904 @default.
- W2918094865 hasAuthorship W2918094865A5068823607 @default.
- W2918094865 hasAuthorship W2918094865A5075414157 @default.
- W2918094865 hasAuthorship W2918094865A5080830950 @default.
- W2918094865 hasAuthorship W2918094865A5089381889 @default.
- W2918094865 hasBestOaLocation W29180948651 @default.
- W2918094865 hasConcept C104317684 @default.
- W2918094865 hasConcept C147483822 @default.
- W2918094865 hasConcept C153911025 @default.
- W2918094865 hasConcept C159047783 @default.
- W2918094865 hasConcept C159654299 @default.
- W2918094865 hasConcept C185592680 @default.
- W2918094865 hasConcept C203014093 @default.
- W2918094865 hasConcept C2778502174 @default.
- W2918094865 hasConcept C40767141 @default.
- W2918094865 hasConcept C542903549 @default.
- W2918094865 hasConcept C54355233 @default.
- W2918094865 hasConcept C553184892 @default.
- W2918094865 hasConcept C55493867 @default.
- W2918094865 hasConcept C81089528 @default.
- W2918094865 hasConcept C81885089 @default.
- W2918094865 hasConcept C86803240 @default.
- W2918094865 hasConceptScore W2918094865C104317684 @default.
- W2918094865 hasConceptScore W2918094865C147483822 @default.