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• W2918383407 startingPage "956" @default.
• W2918383407 abstract "The fresh water microalga Chlamydomonas reinhardtii bioreduced Ag+ to silver nanoparticles (AgNPs) via three biosynthetic routes in a process that could be a more sustainable alternative to conventionally produced AgNPs. The AgNPs were synthesized in either the presence of whole cell cultures, an exopolysaccharide (EPS)-containing cell culture supernatant, or living cells that had been separated from the EPS-containing supernatant and then washed before being suspended again in fresh media. While AgNPs were produced by all three methods, the washed cultures had no supernatant-derived EPS and produced only unstable AgNPs, thus the supernatant-EPS was shown to be necessary to cap and stabilize the biogenic AgNPs. TEM images showed stable AgNPs were mostly spherical and showed a bimodal size distribution about the size ranges of 3.0 ± 1.3 nm and 19.2 ± 5.0 nm for whole cultures and 3.5 ± 0.6 nm and 17.4 ± 2.6 nm for EPS only. Moreover, selected area electron diffraction pattern of these AgNPs confirmed their polycrystalline nature. FTIR of the as-produced AgNPs identified polysaccharides, polyphenols and proteins were responsible for the observed differences in the AgNP stability, size and shape. Additionally, Raman spectroscopy indicated carboxylate and amine groups were bound to the AgNP surface." @default.
• W2918383407 created "2019-03-11" @default.
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• W2918383407 date "2019-03-08" @default.
• W2918383407 modified "2023-10-03" @default.
• W2918383407 title "Individual and Combined Effects of Extracellular Polymeric Substances and Whole Cell Components of Chlamydomonas reinhardtii on Silver Nanoparticle Synthesis and Stability" @default.
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• W2918383407 doi "https://doi.org/10.3390/molecules24050956" @default.
• W2918383407 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6429613" @default.
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