FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2918784045> ?p ?o ?g. }

• W2918784045 abstract "A cutinase from Saccharomonospora viridis AHK190, Cut190, can hydrolyze polyethylene terephthalate and has a unique feature that the activity and stability are regulated by Ca2+ binding. Our recent structural and functional analyses showed three Ca2+ binding sites and their respective roles. Here, we analysed the binding thermodynamics of Mn2+, Zn2+ and Mg2+ to Cut190 and their effects on the catalytic activity and thermal stability. The binding affinities of Mn2+ and Zn2+ were higher than that of Mg2+ and are all entropy driven with a binding stoichiometry of three, one and one for Zn2+, Mn2+ and Mg2+, respectively. The catalytic activity was measured in the presence of the respective metals, where the activity of 0.25 mM Mn2+ was comparable to that of 2.5 mM Ca2+. Our 3D Reference Interaction Site Model calculations suggested that all the ions exhibited a high occupancy rate for Site 2. Thus, Mn2+ and Mg2+ would most likely bind to Site 2 (contributes to stability) with high affinity, while to Sites 1 and 3 (contributes to activity) with low affinity. We elucidate the metal-dependent structural and functional properties of Cut190 and show the subtle balance on structure stability and flexibility is controlled by specific metal ions." @default.
• W2918784045 created "2019-03-11" @default.
• W2918784045 creator A5016590498 @default.
• W2918784045 creator A5037431715 @default.
• W2918784045 creator A5041997474 @default.
• W2918784045 creator A5058425101 @default.
• W2918784045 creator A5065783054 @default.
• W2918784045 creator A5079901164 @default.
• W2918784045 creator A5081898662 @default.
• W2918784045 date "2019-03-02" @default.
• W2918784045 modified "2023-10-12" @default.
• W2918784045 title "Metal binding to cutinase-like enzyme from Saccharomonospora viridis AHK190 and its effects on enzyme activity and stability" @default.
• W2918784045 cites W1580779245 @default.
• W2918784045 cites W1975329681 @default.
• W2918784045 cites W1976499671 @default.
• W2918784045 cites W1979401635 @default.
• W2918784045 cites W1981225934 @default.
• W2918784045 cites W1987161922 @default.
• W2918784045 cites W2004851477 @default.
• W2918784045 cites W2019532760 @default.
• W2918784045 cites W2030664418 @default.
• W2918784045 cites W2042706604 @default.
• W2918784045 cites W2047300683 @default.
• W2918784045 cites W2049991913 @default.
• W2918784045 cites W2093410202 @default.
• W2918784045 cites W2145310386 @default.
• W2918784045 cites W217058915 @default.
• W2918784045 cites W2304982037 @default.
• W2918784045 cites W2517814100 @default.
• W2918784045 cites W2543839061 @default.
• W2918784045 cites W2594021531 @default.
• W2918784045 cites W2600922952 @default.
• W2918784045 cites W2747261161 @default.
• W2918784045 cites W2765971920 @default.
• W2918784045 cites W2806530234 @default.
• W2918784045 cites W2806842034 @default.
• W2918784045 cites W2886639208 @default.
• W2918784045 cites W2892949128 @default.
• W2918784045 doi "https://doi.org/10.1093/jb/mvz020" @default.
• W2918784045 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30825308" @default.
• W2918784045 hasPublicationYear "2019" @default.
• W2918784045 type Work @default.
• W2918784045 sameAs 2918784045 @default.
• W2918784045 citedByCount "3" @default.
• W2918784045 countsByYear W29187840452019 @default.
• W2918784045 countsByYear W29187840452021 @default.
• W2918784045 crossrefType "journal-article" @default.
• W2918784045 hasAuthorship W2918784045A5016590498 @default.
• W2918784045 hasAuthorship W2918784045A5037431715 @default.
• W2918784045 hasAuthorship W2918784045A5041997474 @default.
• W2918784045 hasAuthorship W2918784045A5058425101 @default.
• W2918784045 hasAuthorship W2918784045A5065783054 @default.
• W2918784045 hasAuthorship W2918784045A5079901164 @default.
• W2918784045 hasAuthorship W2918784045A5081898662 @default.
• W2918784045 hasConcept C107824862 @default.
• W2918784045 hasConcept C178790620 @default.
• W2918784045 hasConcept C181199279 @default.
• W2918784045 hasConcept C185592680 @default.
• W2918784045 hasConcept C199164860 @default.
• W2918784045 hasConcept C2777613533 @default.
• W2918784045 hasConcept C2780120296 @default.
• W2918784045 hasConcept C41183919 @default.
• W2918784045 hasConcept C544153396 @default.
• W2918784045 hasConcept C55493867 @default.
• W2918784045 hasConcept C56856141 @default.
• W2918784045 hasConcept C59061564 @default.
• W2918784045 hasConcept C71240020 @default.
• W2918784045 hasConcept C8010536 @default.
• W2918784045 hasConceptScore W2918784045C107824862 @default.
• W2918784045 hasConceptScore W2918784045C178790620 @default.
• W2918784045 hasConceptScore W2918784045C181199279 @default.
• W2918784045 hasConceptScore W2918784045C185592680 @default.
• W2918784045 hasConceptScore W2918784045C199164860 @default.
• W2918784045 hasConceptScore W2918784045C2777613533 @default.
• W2918784045 hasConceptScore W2918784045C2780120296 @default.
• W2918784045 hasConceptScore W2918784045C41183919 @default.
• W2918784045 hasConceptScore W2918784045C544153396 @default.
• W2918784045 hasConceptScore W2918784045C55493867 @default.
• W2918784045 hasConceptScore W2918784045C56856141 @default.
• W2918784045 hasConceptScore W2918784045C59061564 @default.
• W2918784045 hasConceptScore W2918784045C71240020 @default.
• W2918784045 hasConceptScore W2918784045C8010536 @default.
• W2918784045 hasFunder F4320311405 @default.
• W2918784045 hasFunder F4320334764 @default.
• W2918784045 hasLocation W29187840451 @default.
• W2918784045 hasLocation W29187840452 @default.
• W2918784045 hasOpenAccess W2918784045 @default.
• W2918784045 hasPrimaryLocation W29187840451 @default.
• W2918784045 hasRelatedWork W2025566860 @default.
• W2918784045 hasRelatedWork W2084354306 @default.
• W2918784045 hasRelatedWork W2334511488 @default.
• W2918784045 hasRelatedWork W2352203881 @default.
• W2918784045 hasRelatedWork W2354067357 @default.
• W2918784045 hasRelatedWork W2357451662 @default.
• W2918784045 hasRelatedWork W2392537197 @default.
• W2918784045 hasRelatedWork W2759274657 @default.
• W2918784045 hasRelatedWork W4249294104 @default.
• W2918784045 hasRelatedWork W2775064938 @default.
• W2918784045 isParatext "false" @default.
• W2918784045 isRetracted "false" @default.

• next