FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2919723712> ?p ?o ?g. }

• W2919723712 endingPage "1763" @default.
• W2919723712 startingPage "1745" @default.
• W2919723712 abstract "Background and Purpose Although protein phosphatases regulate multiple cellular functions, their modulation under hypoxia remains unclear. We investigated expression of the protein phosphatase system under normoxic/hypoxic conditions and the mechanism by which hypoxia alters protein phosphatase 2A (PP2A) activity. Experimental Approach Human cardiovascular cells were cultured in cell type specific media under normoxic or hypoxic conditions (1% O 2 ). Effects on mRNA expression, phosphatase activity, post‐translational modification, and involvement of hypoxia inducible factor 1α (HIF‐1α) were assessed using RT‐PCR, immunoblotting, an activity assay, and siRNA silencing. Key Results All components of the protein phosphatase system studied were expressed in each cell line. Hypoxia attenuated mRNA expression of the transcripts in a cell line‐ and time‐dependent manner. In human aortic smooth muscle cells (HASMC) and AC16 cells, hypoxia decreased PP2Ac activity and mRNA expression without altering PP2Ac abundance. Hypoxia increased demethylated PP2Ac (DPP2Ac) and phosphatase methylesterase 1 (PME‐1) abundance but decreased leucine carboxyl methyltransferase 1 (LCMT‐1) abundance. HIF‐1α siRNA prevented the hypoxia‐mediated decrease in phosphatase activity and expression of the catalytic subunit of protein phosphatase 2A (PPP2CA), independently of altering pPP2Ac, DPP2Ac, LCMT‐1, or PME‐1 abundance. Conclusion and Implications Cardiovascular cells express multiple components of the PP2A system. In HASMC and AC16 cells, hypoxia inhibits PP2A activity through HIF‐1α‐dependent and ‐independent mechanisms, with the latter being consistent with altered PP2A holoenzyme assembly. This indicates a complex inhibitory effect of hypoxia on the PP2A system, and highlights PP2A as a therapeutic target for diseases associated with dysregulated protein phosphorylation." @default.
• W2919723712 created "2019-03-11" @default.
• W2919723712 creator A5062836082 @default.
• W2919723712 creator A5086504073 @default.
• W2919723712 date "2019-04-22" @default.
• W2919723712 modified "2023-10-13" @default.
• W2919723712 title "Hypoxia modulates protein phosphatase 2A through HIF‐1α dependent and independent mechanisms in human aortic smooth muscle cells and ventricular cardiomyocytes" @default.
• W2919723712 cites W1490748999 @default.
• W2919723712 cites W1812584421 @default.
• W2919723712 cites W1819171550 @default.
• W2919723712 cites W1973881801 @default.
• W2919723712 cites W1976313499 @default.
• W2919723712 cites W1977599918 @default.
• W2919723712 cites W1984128917 @default.
• W2919723712 cites W1988862655 @default.
• W2919723712 cites W1995047831 @default.
• W2919723712 cites W1997664222 @default.
• W2919723712 cites W1999560753 @default.
• W2919723712 cites W2010332019 @default.
• W2919723712 cites W2010386340 @default.
• W2919723712 cites W2016687512 @default.
• W2919723712 cites W2026508325 @default.
• W2919723712 cites W2027255839 @default.
• W2919723712 cites W2030880839 @default.
• W2919723712 cites W2032137122 @default.
• W2919723712 cites W2032896827 @default.
• W2919723712 cites W2033587029 @default.
• W2919723712 cites W2046958672 @default.
• W2919723712 cites W2054574453 @default.
• W2919723712 cites W2057593147 @default.
• W2919723712 cites W2062158492 @default.
• W2919723712 cites W2070681674 @default.
• W2919723712 cites W2071143412 @default.
• W2919723712 cites W2079745507 @default.
• W2919723712 cites W2085041795 @default.
• W2919723712 cites W2085078083 @default.
• W2919723712 cites W2091466873 @default.
• W2919723712 cites W2097607584 @default.
• W2919723712 cites W2101201744 @default.
• W2919723712 cites W2108474070 @default.
• W2919723712 cites W2112049656 @default.
• W2919723712 cites W2117408406 @default.
• W2919723712 cites W2117767094 @default.
• W2919723712 cites W2120553816 @default.
• W2919723712 cites W2122530165 @default.
• W2919723712 cites W2122653610 @default.
• W2919723712 cites W2134827458 @default.
• W2919723712 cites W2139836283 @default.
• W2919723712 cites W2150541721 @default.
• W2919723712 cites W2159527263 @default.
• W2919723712 cites W2223519024 @default.
• W2919723712 cites W2224592948 @default.
• W2919723712 cites W2302002701 @default.
• W2919723712 cites W2405438293 @default.
• W2919723712 cites W2431250996 @default.
• W2919723712 cites W2522883401 @default.
• W2919723712 cites W2550469153 @default.
• W2919723712 cites W2568675678 @default.
• W2919723712 cites W2588464299 @default.
• W2919723712 cites W2600756322 @default.
• W2919723712 cites W2606954735 @default.
• W2919723712 cites W2740928018 @default.
• W2919723712 cites W2753083403 @default.
• W2919723712 cites W2765684626 @default.
• W2919723712 cites W2766306821 @default.
• W2919723712 cites W2771328536 @default.
• W2919723712 cites W2884790554 @default.
• W2919723712 cites W2899037189 @default.
• W2919723712 cites W632054478 @default.
• W2919723712 doi "https://doi.org/10.1111/bph.14648" @default.
• W2919723712 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6514298" @default.
• W2919723712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30825189" @default.
• W2919723712 hasPublicationYear "2019" @default.
• W2919723712 type Work @default.
• W2919723712 sameAs 2919723712 @default.
• W2919723712 citedByCount "10" @default.
• W2919723712 countsByYear W29197237122019 @default.
• W2919723712 countsByYear W29197237122020 @default.
• W2919723712 countsByYear W29197237122021 @default.
• W2919723712 countsByYear W29197237122022 @default.
• W2919723712 countsByYear W29197237122023 @default.
• W2919723712 crossrefType "journal-article" @default.
• W2919723712 hasAuthorship W2919723712A5062836082 @default.
• W2919723712 hasAuthorship W2919723712A5086504073 @default.
• W2919723712 hasBestOaLocation W29197237121 @default.
• W2919723712 hasConcept C104292427 @default.
• W2919723712 hasConcept C104317684 @default.
• W2919723712 hasConcept C105580179 @default.
• W2919723712 hasConcept C119056186 @default.
• W2919723712 hasConcept C11960822 @default.
• W2919723712 hasConcept C153911025 @default.
• W2919723712 hasConcept C160160445 @default.
• W2919723712 hasConcept C178666793 @default.
• W2919723712 hasConcept C178790620 @default.
• W2919723712 hasConcept C181199279 @default.
• W2919723712 hasConcept C185592680 @default.
• W2919723712 hasConcept C2779408958 @default.
• W2919723712 hasConcept C389152 @default.

• next