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- W2920165086 abstract "We used modofied Koh method to cultivate the primary neurons of newborn rat. Evaluation of neurons was made by immunocytochemical techniques. Then, the neurons were incubated with AP4-Netrin 4 fusion protein and localization was performed by in situ staining of AP. Cultured neurons were stick to the wall of the cultivation plate when they were growing. The neurons were satiety and with long axon. Immunocytochmical staining showed 90% neurofilament positive staining cell. In situ staining of AP4-Netrin 4 protein bound to neurons showed that Netrin 4 receptor was on the mebrane. Primary culture of olfactory and cortical neurons of newborn rat by the method is a reliable and highly stable way to obtain neurons. This neuronal culture is a useful model for moleculo-biological and neuronbiological studies." @default.
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- W2920165086 title "Primary culture of olfactory bulb and cortical neurons of newborn rat and primary study of the localization of Netrin 4 receptor on the neurons" @default.
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