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- W2920174847 abstract "A crucial feature of differentiated cells is the rapid activation of enhancer-driven transcriptional programs in response to signals. The potential contributions of physicochemical properties of enhancer assembly in signaling events remain poorly understood. Here we report that in human breast cancer cells, the acute 17β-estradiol-dependent activation of functional enhancers requires assembly of an enhancer RNA–dependent ribonucleoprotein (eRNP) complex exhibiting properties of phase-separated condensates. Unexpectedly, while acute ligand-dependent assembly of eRNPs resulted in enhancer activation sensitive to chemical disruption of phase separation, chronically activated enhancers proved resistant to such disruption, with progressive maturation of eRNPs to a more gel-like state. Acute, but not chronic, stimulation resulted in ligand-induced, condensin-dependent changes in spatial chromatin conformation based on homotypic enhancer association, resulting in cooperative enhancer-activation events. Thus, distinct physicochemical properties of eRNP condensates on enhancers serve as determinants of rapid ligand-dependent alterations in chromosomal architecture and cooperative enhancer activation. After acute agonist stimulation, phase-separated complexes form at estrogen-receptor-bound enhancer sites and coalesce into condensates with cooperative enhancer activity. Chronic stimulation causes the condensates to mature into a less dynamic, gel-like state." @default.
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- W2920174847 date "2019-03-01" @default.
- W2920174847 modified "2023-10-14" @default.
- W2920174847 title "Phase separation of ligand-activated enhancers licenses cooperative chromosomal enhancer assembly" @default.
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- W2920174847 doi "https://doi.org/10.1038/s41594-019-0190-5" @default.
- W2920174847 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6709854" @default.
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- W2920174847 hasPublicationYear "2019" @default.
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