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- W2920346076 abstract "We determined the diagnostic value of the detection of specific antibodies against various Angiostrongylus vasorum antigens by ELISA. Also, A. vasorum adult somatic antigen purified by monoclonal antibodies was evaluated in two sandwich-ELISAs. Among the crude antigens, the best sensitivities when testing naturally infected dogs were obtained using adult excretory/secretory (E/S) antigen (85.7%) and adult somatic antigen (76.2%), both yielding results comparable to the sandwich-ELISA (81%). In experimentally inoculated dogs, the sensitivities ranged from 97.7 to 100%. The specificity was 98.8% with all ELISAs. Cross-reactions using adult somatic, adult E/S and L1 somatic antigen were observed in sera of dogs infected with Crenosoma vulpis, Dirofilaria immitis, D. repens or Eucoleus aerophilous. In contrast, using the mAb-purified antigens, cross-reactions were minimal. Specific antibodies were detected starting between 13 and 21 days post experimental inoculation. After anthelmintic treatment, the animals became seronegative within 2-9 weeks. Serology represents an alternative method for individual diagnosis and for follow-up investigations after anthelmintic treatment, and it is a useful tool for epidemiological investigations on a population level. Wir bestimmten den diagnostischen Wert des Nachweises spezifischer Antikorper gegen verschiedene Angiostrongylus vasorum-Antigene im ELISA. In einem Sandwich-ELISA wurde zudem ein somatisches A. vasorum-Adultantigen evaluiert, welches mit monoklonalen Antikorpern gereinigt wurde. Bei naturlich infizierten Hunden wurden bei Verwendung von Rohantigenen die hochsten Sensitivitaten mit adultem exkretorischen/sekretorischen (E/S) (85.7%) und adultem somatischen Antigen (76.2%) erreicht, welche beide mit den zwei Sandwich-ELISAs (81%) vergleichbare Resultate lieferten. Bei experimentell infizierten Hunden lag die Sensitivitat zwischen 97.7 und 100%. Die Spezifitat lag in allen ELISAs bei 98.8%. Bei der Verwendung des adulten somatischen Antigens, des adulten E/S Antigens, sowie des somatischen L1-Antigens wurden Kreuzreaktionen bei Seren von Hunden beobachtet, welche mit Crenosoma vulpis, Dirofilaria immitis, D. repens oder Eucoleus aerophilus infiziert waren. Im Gegensatz dazu waren Kreuzreaktionen im Sandwich-ELISA minimal. Spezifische Antikorper konnten bereits zwischen 13 und 21 Tagen nach experimenteller Infektion nachgewiesen werden. Nach einer anthelminthischen Behandlung wurden die Hunde innerhalb von 2-9 Wochen seronegativ. Die Serologie stellt eine Alternative dar fur die individuelle Diagnose und fur Verlaufs-Untersuchungen nach anthelminthischer Behandlung, und sie eignet sich auch fur Populationsuntersuchungen in epidemiologischen Studien." @default.
- W2920346076 created "2019-03-11" @default.
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- W2920346076 date "2012-01-01" @default.
- W2920346076 modified "2023-09-27" @default.
- W2920346076 title "Detection of specific antibodies in dogs with Angiostrongylus vasorum" @default.
- W2920346076 doi "https://doi.org/10.5167/uzh-63177" @default.
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