Matches in SemOpenAlex for { <https://semopenalex.org/work/W2920467501> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2920467501 endingPage "79" @default.
- W2920467501 startingPage "76" @default.
- W2920467501 abstract "Locating the antigenic determinant(epitope)of allergenic protein TB22 of tartary buckwheat, to elucidate the relative allergenic mechanism and lay the foundation for molecular modification and breeding improvement, it is essential to obtain large amount of purified protein in microbes. The TB22 gene was cloned into vector pQE-31 and expressed as inclusion body in host strain M15 after induced by IPTG at 37℃. Western Blotting test proved that the Histidine tag has been attached to the N-terminal of the expressed protein. The target protein accounted for over 40% of total precipitated protein after preliminarily purified by 8mol/L urea and finally reached to above 90% by Hitrap Chelating HP affinity chromatography. An effective purification method of recombinant TB22 was built and the purified inclusion body was obtained also, which laid the foundation for the following preparation of anti-TB22 antibody and antigenic determinant research." @default.
- W2920467501 created "2019-03-11" @default.
- W2920467501 creator A5041448915 @default.
- W2920467501 creator A5044954133 @default.
- W2920467501 creator A5063551023 @default.
- W2920467501 creator A5068534508 @default.
- W2920467501 creator A5088888083 @default.
- W2920467501 date "2003-01-01" @default.
- W2920467501 modified "2023-09-23" @default.
- W2920467501 title "Prokaryotic Expression and Purification of Allergenic Protein TB22 of Tartary Buckwheat" @default.
- W2920467501 hasPublicationYear "2003" @default.
- W2920467501 type Work @default.
- W2920467501 sameAs 2920467501 @default.
- W2920467501 citedByCount "0" @default.
- W2920467501 crossrefType "journal-article" @default.
- W2920467501 hasAuthorship W2920467501A5041448915 @default.
- W2920467501 hasAuthorship W2920467501A5044954133 @default.
- W2920467501 hasAuthorship W2920467501A5063551023 @default.
- W2920467501 hasAuthorship W2920467501A5068534508 @default.
- W2920467501 hasAuthorship W2920467501A5088888083 @default.
- W2920467501 hasConcept C104317684 @default.
- W2920467501 hasConcept C147483822 @default.
- W2920467501 hasConcept C153911025 @default.
- W2920467501 hasConcept C159654299 @default.
- W2920467501 hasConcept C170344550 @default.
- W2920467501 hasConcept C179247698 @default.
- W2920467501 hasConcept C181199279 @default.
- W2920467501 hasConcept C185592680 @default.
- W2920467501 hasConcept C195616568 @default.
- W2920467501 hasConcept C203750385 @default.
- W2920467501 hasConcept C33161422 @default.
- W2920467501 hasConcept C40767141 @default.
- W2920467501 hasConcept C54355233 @default.
- W2920467501 hasConcept C55493867 @default.
- W2920467501 hasConcept C85265743 @default.
- W2920467501 hasConcept C86803240 @default.
- W2920467501 hasConceptScore W2920467501C104317684 @default.
- W2920467501 hasConceptScore W2920467501C147483822 @default.
- W2920467501 hasConceptScore W2920467501C153911025 @default.
- W2920467501 hasConceptScore W2920467501C159654299 @default.
- W2920467501 hasConceptScore W2920467501C170344550 @default.
- W2920467501 hasConceptScore W2920467501C179247698 @default.
- W2920467501 hasConceptScore W2920467501C181199279 @default.
- W2920467501 hasConceptScore W2920467501C185592680 @default.
- W2920467501 hasConceptScore W2920467501C195616568 @default.
- W2920467501 hasConceptScore W2920467501C203750385 @default.
- W2920467501 hasConceptScore W2920467501C33161422 @default.
- W2920467501 hasConceptScore W2920467501C40767141 @default.
- W2920467501 hasConceptScore W2920467501C54355233 @default.
- W2920467501 hasConceptScore W2920467501C55493867 @default.
- W2920467501 hasConceptScore W2920467501C85265743 @default.
- W2920467501 hasConceptScore W2920467501C86803240 @default.
- W2920467501 hasIssue "11" @default.
- W2920467501 hasLocation W29204675011 @default.
- W2920467501 hasOpenAccess W2920467501 @default.
- W2920467501 hasPrimaryLocation W29204675011 @default.
- W2920467501 hasRelatedWork W1514415175 @default.
- W2920467501 hasRelatedWork W2012117922 @default.
- W2920467501 hasRelatedWork W2025800051 @default.
- W2920467501 hasRelatedWork W2058825595 @default.
- W2920467501 hasRelatedWork W2085792173 @default.
- W2920467501 hasRelatedWork W2098328030 @default.
- W2920467501 hasRelatedWork W2361011428 @default.
- W2920467501 hasRelatedWork W2361637742 @default.
- W2920467501 hasRelatedWork W2389556740 @default.
- W2920467501 hasRelatedWork W2404783165 @default.
- W2920467501 hasRelatedWork W2411211624 @default.
- W2920467501 hasRelatedWork W2418247666 @default.
- W2920467501 hasRelatedWork W2462878231 @default.
- W2920467501 hasRelatedWork W2767648121 @default.
- W2920467501 hasRelatedWork W2960089222 @default.
- W2920467501 hasRelatedWork W2990861945 @default.
- W2920467501 hasRelatedWork W3142340049 @default.
- W2920467501 hasRelatedWork W3152350177 @default.
- W2920467501 hasRelatedWork W2097669783 @default.
- W2920467501 hasRelatedWork W2270760637 @default.
- W2920467501 hasVolume "23" @default.
- W2920467501 isParatext "false" @default.
- W2920467501 isRetracted "false" @default.
- W2920467501 magId "2920467501" @default.
- W2920467501 workType "article" @default.