Matches in SemOpenAlex for { <https://semopenalex.org/work/W2920495229> ?p ?o ?g. }
- W2920495229 endingPage "e1006213" @default.
- W2920495229 startingPage "e1006213" @default.
- W2920495229 abstract "Understanding the fundamental characteristics of microbial communities could have far reaching implications for human health and applied biotechnology. Despite this, much is still unknown regarding the genetic basis and evolutionary strategies underlying the formation of viable synthetic communities. By pairing auxotrophic mutants in co-culture, it has been demonstrated that viable nascent E. coli communities can be established where the mutant strains are metabolically coupled. A novel algorithm, OptAux, was constructed to design 61 unique multi-knockout E. coli auxotrophic strains that require significant metabolite uptake to grow. These predicted knockouts included a diverse set of novel non-specific auxotrophs that result from inhibition of major biosynthetic subsystems. Three OptAux predicted non-specific auxotrophic strains—with diverse metabolic deficiencies—were co-cultured with an L-histidine auxotroph and optimized via adaptive laboratory evolution (ALE). Time-course sequencing revealed the genetic changes employed by each strain to achieve higher community growth rates and provided insight into mechanisms for adapting to the syntrophic niche. A community model of metabolism and gene expression was utilized to predict the relative community composition and fundamental characteristics of the evolved communities. This work presents new insight into the genetic strategies underlying viable nascent community formation and a cutting-edge computational method to elucidate metabolic changes that empower the creation of cooperative communities." @default.
- W2920495229 created "2019-03-11" @default.
- W2920495229 creator A5002373415 @default.
- W2920495229 creator A5008123229 @default.
- W2920495229 creator A5022020022 @default.
- W2920495229 creator A5028654663 @default.
- W2920495229 creator A5033881627 @default.
- W2920495229 creator A5048081015 @default.
- W2920495229 creator A5051432302 @default.
- W2920495229 creator A5062042072 @default.
- W2920495229 creator A5066195302 @default.
- W2920495229 creator A5069073018 @default.
- W2920495229 creator A5072991489 @default.
- W2920495229 creator A5073232270 @default.
- W2920495229 creator A5075454403 @default.
- W2920495229 creator A5082559708 @default.
- W2920495229 date "2019-03-01" @default.
- W2920495229 modified "2023-10-14" @default.
- W2920495229 title "The genetic basis for adaptation of model-designed syntrophic co-cultures" @default.
- W2920495229 cites W1509674904 @default.
- W2920495229 cites W1535567480 @default.
- W2920495229 cites W1546232003 @default.
- W2920495229 cites W1602357967 @default.
- W2920495229 cites W1628821170 @default.
- W2920495229 cites W1824535033 @default.
- W2920495229 cites W1828505669 @default.
- W2920495229 cites W1832792590 @default.
- W2920495229 cites W1889289315 @default.
- W2920495229 cites W1893986342 @default.
- W2920495229 cites W1955448153 @default.
- W2920495229 cites W1958250075 @default.
- W2920495229 cites W1964832282 @default.
- W2920495229 cites W1967677046 @default.
- W2920495229 cites W1968033606 @default.
- W2920495229 cites W1970559599 @default.
- W2920495229 cites W1974662378 @default.
- W2920495229 cites W1975597731 @default.
- W2920495229 cites W1982013068 @default.
- W2920495229 cites W1984445372 @default.
- W2920495229 cites W1991509434 @default.
- W2920495229 cites W1998240064 @default.
- W2920495229 cites W2005931802 @default.
- W2920495229 cites W2022616740 @default.
- W2920495229 cites W2022720960 @default.
- W2920495229 cites W2028974656 @default.
- W2920495229 cites W2029403511 @default.
- W2920495229 cites W2029764374 @default.
- W2920495229 cites W2029945156 @default.
- W2920495229 cites W2038168681 @default.
- W2920495229 cites W2041500621 @default.
- W2920495229 cites W2047389560 @default.
- W2920495229 cites W2048465561 @default.
- W2920495229 cites W2048950596 @default.
- W2920495229 cites W2056747353 @default.
- W2920495229 cites W2060566975 @default.
- W2920495229 cites W2076393137 @default.
- W2920495229 cites W2085661494 @default.
- W2920495229 cites W2090971499 @default.
- W2920495229 cites W2097113949 @default.
- W2920495229 cites W2107349404 @default.
- W2920495229 cites W2108234281 @default.
- W2920495229 cites W2108595206 @default.
- W2920495229 cites W2108656223 @default.
- W2920495229 cites W2109251637 @default.
- W2920495229 cites W2111967267 @default.
- W2920495229 cites W2113497222 @default.
- W2920495229 cites W2113651242 @default.
- W2920495229 cites W2114064051 @default.
- W2920495229 cites W2114270359 @default.
- W2920495229 cites W2121427573 @default.
- W2920495229 cites W2122093325 @default.
- W2920495229 cites W2122860350 @default.
- W2920495229 cites W2124737787 @default.
- W2920495229 cites W2126175129 @default.
- W2920495229 cites W2126999541 @default.
- W2920495229 cites W2128710055 @default.
- W2920495229 cites W2129087744 @default.
- W2920495229 cites W2129890743 @default.
- W2920495229 cites W2130335772 @default.
- W2920495229 cites W2132473766 @default.
- W2920495229 cites W2142026735 @default.
- W2920495229 cites W2146134224 @default.
- W2920495229 cites W2148520571 @default.
- W2920495229 cites W2149001103 @default.
- W2920495229 cites W2152625710 @default.
- W2920495229 cites W2163949203 @default.
- W2920495229 cites W2167708455 @default.
- W2920495229 cites W2170298003 @default.
- W2920495229 cites W2170551349 @default.
- W2920495229 cites W2181523240 @default.
- W2920495229 cites W2224056471 @default.
- W2920495229 cites W2271440286 @default.
- W2920495229 cites W2290768068 @default.
- W2920495229 cites W2293953879 @default.
- W2920495229 cites W2397950241 @default.
- W2920495229 cites W2402388476 @default.
- W2920495229 cites W2416065856 @default.
- W2920495229 cites W2521256720 @default.