Matches in SemOpenAlex for { <https://semopenalex.org/work/W2920536551> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2920536551 abstract "Background. The aim of this study is to measure global 5-methylcystosine (5MeC) methylation in paraffin embedded prostatic tissues and cell lines using flow cytometry. Methods. Cell/nuclei suspension from 10 cases of benign prostatic hyperplasia (BPH), 10 cases of prostatic adenocarcinoma, and two prostatic cell lines (PNT1A and LNCaP) were prepared using modified heat pretreatment technique. 5MeC global methylation was assessed by flow cytometry of cell/nuclei suspension and immunostaining of tissue sections. Results. Higher percentage of positively stained cells (PPSC) and mean channel fluorescence (MCF) were detected in PNT1A cell line and BPH cell/nuclei suspensions as compared to LNCaP cell lines and adenocarcinoma cell/nuclei suspensions. Lower scores of 5MeC immunostaining were observed in all prostate adenocarcinoma tissue sections as compared to BPH sections indicating global hypomethylation in prostate adenocarcinoma. Two distinctive populations of cells were detected in histograms generated from most of the BPH cell/nuclei suspensions. Conclusion. The study developed a novel technique that could measure 5MeC global methylation in paraffin embedded prostatic tissues. This represents a rapid and objective assessment of methylation and when combined with tissue micro-dissection and cell sorting, this technique could be applied to larger tissue samples such as post radical prostatectomy and transurethral resected specimens." @default.
- W2920536551 created "2019-03-11" @default.
- W2920536551 creator A5025866705 @default.
- W2920536551 creator A5057531245 @default.
- W2920536551 creator A5086671850 @default.
- W2920536551 date "2018-03-20" @default.
- W2920536551 modified "2023-09-26" @default.
- W2920536551 title "Assessment of Global Methylation in Paraffin Embedded Prostatic Tissues and Cell Lines Using Flow Cytometry" @default.
- W2920536551 doi "https://doi.org/10.31377/ammr.v1i1.495" @default.
- W2920536551 hasPublicationYear "2018" @default.
- W2920536551 type Work @default.
- W2920536551 sameAs 2920536551 @default.
- W2920536551 citedByCount "0" @default.
- W2920536551 crossrefType "journal-article" @default.
- W2920536551 hasAuthorship W2920536551A5025866705 @default.
- W2920536551 hasAuthorship W2920536551A5057531245 @default.
- W2920536551 hasAuthorship W2920536551A5086671850 @default.
- W2920536551 hasBestOaLocation W29205365511 @default.
- W2920536551 hasConcept C104317684 @default.
- W2920536551 hasConcept C121608353 @default.
- W2920536551 hasConcept C126322002 @default.
- W2920536551 hasConcept C142724271 @default.
- W2920536551 hasConcept C1491633281 @default.
- W2920536551 hasConcept C153911025 @default.
- W2920536551 hasConcept C185592680 @default.
- W2920536551 hasConcept C204232928 @default.
- W2920536551 hasConcept C2776235491 @default.
- W2920536551 hasConcept C2776438761 @default.
- W2920536551 hasConcept C2777562237 @default.
- W2920536551 hasConcept C2779466945 @default.
- W2920536551 hasConcept C2779723316 @default.
- W2920536551 hasConcept C2781182431 @default.
- W2920536551 hasConcept C33288867 @default.
- W2920536551 hasConcept C4224716 @default.
- W2920536551 hasConcept C502942594 @default.
- W2920536551 hasConcept C54355233 @default.
- W2920536551 hasConcept C553184892 @default.
- W2920536551 hasConcept C55493867 @default.
- W2920536551 hasConcept C71924100 @default.
- W2920536551 hasConcept C81885089 @default.
- W2920536551 hasConcept C86803240 @default.
- W2920536551 hasConceptScore W2920536551C104317684 @default.
- W2920536551 hasConceptScore W2920536551C121608353 @default.
- W2920536551 hasConceptScore W2920536551C126322002 @default.
- W2920536551 hasConceptScore W2920536551C142724271 @default.
- W2920536551 hasConceptScore W2920536551C1491633281 @default.
- W2920536551 hasConceptScore W2920536551C153911025 @default.
- W2920536551 hasConceptScore W2920536551C185592680 @default.
- W2920536551 hasConceptScore W2920536551C204232928 @default.
- W2920536551 hasConceptScore W2920536551C2776235491 @default.
- W2920536551 hasConceptScore W2920536551C2776438761 @default.
- W2920536551 hasConceptScore W2920536551C2777562237 @default.
- W2920536551 hasConceptScore W2920536551C2779466945 @default.
- W2920536551 hasConceptScore W2920536551C2779723316 @default.
- W2920536551 hasConceptScore W2920536551C2781182431 @default.
- W2920536551 hasConceptScore W2920536551C33288867 @default.
- W2920536551 hasConceptScore W2920536551C4224716 @default.
- W2920536551 hasConceptScore W2920536551C502942594 @default.
- W2920536551 hasConceptScore W2920536551C54355233 @default.
- W2920536551 hasConceptScore W2920536551C553184892 @default.
- W2920536551 hasConceptScore W2920536551C55493867 @default.
- W2920536551 hasConceptScore W2920536551C71924100 @default.
- W2920536551 hasConceptScore W2920536551C81885089 @default.
- W2920536551 hasConceptScore W2920536551C86803240 @default.
- W2920536551 hasLocation W29205365511 @default.
- W2920536551 hasOpenAccess W2920536551 @default.
- W2920536551 hasPrimaryLocation W29205365511 @default.
- W2920536551 hasRelatedWork W1578440178 @default.
- W2920536551 hasRelatedWork W1965936810 @default.
- W2920536551 hasRelatedWork W1994515912 @default.
- W2920536551 hasRelatedWork W1997087627 @default.
- W2920536551 hasRelatedWork W2023599261 @default.
- W2920536551 hasRelatedWork W2031709263 @default.
- W2920536551 hasRelatedWork W2032416309 @default.
- W2920536551 hasRelatedWork W2048248290 @default.
- W2920536551 hasRelatedWork W2058163450 @default.
- W2920536551 hasRelatedWork W2079685816 @default.
- W2920536551 hasRelatedWork W2147779944 @default.
- W2920536551 hasRelatedWork W2327802513 @default.
- W2920536551 hasRelatedWork W2332191975 @default.
- W2920536551 hasRelatedWork W2341592614 @default.
- W2920536551 hasRelatedWork W2402748035 @default.
- W2920536551 hasRelatedWork W2464985916 @default.
- W2920536551 hasRelatedWork W2474985844 @default.
- W2920536551 hasRelatedWork W2733040513 @default.
- W2920536551 hasRelatedWork W2898687107 @default.
- W2920536551 hasRelatedWork W437264737 @default.
- W2920536551 isParatext "false" @default.
- W2920536551 isRetracted "false" @default.
- W2920536551 magId "2920536551" @default.
- W2920536551 workType "article" @default.