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- W2920613080 abstract "Super-resolution microscopy techniques enable optical imaging in live cells with unprecedented spatial resolution. They unfortunately lack the temporal resolution required to directly investigate cellular dynamics at scales sufficient to measure molecular diffusion. These fast time scales are, on the other hand, routinely accessible by spectroscopic techniques such as fluorescence correlation spectroscopy (FCS). To enable the direct investigation of fast dynamics at the relevant spatial scales, FCS has been combined with super-resolution stimulated emission depletion (STED) microscopy. STED-FCS has been applied in point or scanning mode to reveal nanoscale diffusion behavior of molecules in live cells. In this protocol, we describe the technical details of performing point STED-FCS (pSTED-FCS) and scanning STED-FCS (sSTED-FCS) measurements, from calibration and sample preparation to data acquisition and analysis. We give particular emphasis to 2D diffusion dynamics in cellular membranes, using molecules tagged with organic fluorophores. These measurements can be accomplished within 4-6 h by those proficient in fluorescence imaging." @default.
- W2920613080 created "2019-03-11" @default.
- W2920613080 creator A5002316926 @default.
- W2920613080 creator A5029490828 @default.
- W2920613080 creator A5046989784 @default.
- W2920613080 creator A5058514738 @default.
- W2920613080 creator A5067171744 @default.
- W2920613080 creator A5069417812 @default.
- W2920613080 creator A5073935293 @default.
- W2920613080 date "2019-03-06" @default.
- W2920613080 modified "2023-10-01" @default.
- W2920613080 title "Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED–FCS" @default.
- W2920613080 cites W1665694733 @default.
- W2920613080 cites W1748288754 @default.
- W2920613080 cites W1930766016 @default.
- W2920613080 cites W1965180796 @default.
- W2920613080 cites W1965238080 @default.
- W2920613080 cites W1967640331 @default.
- W2920613080 cites W1972234131 @default.
- W2920613080 cites W1974642741 @default.
- W2920613080 cites W1978249718 @default.
- W2920613080 cites W1978250115 @default.
- W2920613080 cites W1979911163 @default.
- W2920613080 cites W1981916955 @default.
- W2920613080 cites W1982051519 @default.
- W2920613080 cites W1982859702 @default.
- W2920613080 cites W1998053479 @default.
- W2920613080 cites W2001274423 @default.
- W2920613080 cites W2002832176 @default.
- W2920613080 cites W2008264883 @default.
- W2920613080 cites W2013990977 @default.
- W2920613080 cites W2015832765 @default.
- W2920613080 cites W2016036293 @default.
- W2920613080 cites W2018333808 @default.
- W2920613080 cites W2021882008 @default.
- W2920613080 cites W2022393754 @default.
- W2920613080 cites W2022903803 @default.
- W2920613080 cites W2023834823 @default.
- W2920613080 cites W2030014489 @default.
- W2920613080 cites W2030863049 @default.
- W2920613080 cites W2031032645 @default.
- W2920613080 cites W2032003671 @default.
- W2920613080 cites W2038202291 @default.
- W2920613080 cites W2044600486 @default.
- W2920613080 cites W2046321737 @default.
- W2920613080 cites W2050719403 @default.
- W2920613080 cites W2057062200 @default.
- W2920613080 cites W2057141116 @default.
- W2920613080 cites W2063093669 @default.
- W2920613080 cites W2070380977 @default.
- W2920613080 cites W2078366572 @default.
- W2920613080 cites W2088381564 @default.
- W2920613080 cites W2089355821 @default.
- W2920613080 cites W2089988249 @default.
- W2920613080 cites W2101873758 @default.
- W2920613080 cites W2103944364 @default.
- W2920613080 cites W2115265985 @default.
- W2920613080 cites W2115737236 @default.
- W2920613080 cites W2120849439 @default.
- W2920613080 cites W2124967991 @default.
- W2920613080 cites W2130261475 @default.
- W2920613080 cites W2133559641 @default.
- W2920613080 cites W2134178604 @default.
- W2920613080 cites W2136133782 @default.
- W2920613080 cites W2138773137 @default.
- W2920613080 cites W2141829222 @default.
- W2920613080 cites W2167279371 @default.
- W2920613080 cites W2192142078 @default.
- W2920613080 cites W2193995799 @default.
- W2920613080 cites W2210154426 @default.
- W2920613080 cites W2253838293 @default.
- W2920613080 cites W2299243614 @default.
- W2920613080 cites W2300023655 @default.
- W2920613080 cites W2410070880 @default.
- W2920613080 cites W2418877647 @default.
- W2920613080 cites W2510778908 @default.
- W2920613080 cites W2513825440 @default.
- W2920613080 cites W2573984120 @default.
- W2920613080 cites W2587132439 @default.
- W2920613080 cites W2599649240 @default.
- W2920613080 cites W2605759836 @default.
- W2920613080 cites W2613527097 @default.
- W2920613080 cites W2729283896 @default.
- W2920613080 cites W2743153858 @default.
- W2920613080 cites W2751886572 @default.
- W2920613080 cites W2752919824 @default.
- W2920613080 cites W2753791829 @default.
- W2920613080 cites W2756232354 @default.
- W2920613080 cites W2756299032 @default.
- W2920613080 cites W2768096711 @default.
- W2920613080 cites W2769509299 @default.
- W2920613080 cites W2773468850 @default.
- W2920613080 cites W2775616627 @default.
- W2920613080 cites W2776366088 @default.
- W2920613080 cites W2776544823 @default.
- W2920613080 cites W2782890832 @default.
- W2920613080 cites W2791176879 @default.
- W2920613080 cites W2792000794 @default.
- W2920613080 cites W2793510372 @default.
- W2920613080 cites W2883057275 @default.