FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2920778523> ?p ?o ?g. }

• W2920778523 abstract "Purpose: Emerging evidence suggests that 27-Hydroxycholesterol (27-OHC) causes neurodegenerative diseases through induction of cytotoxicity and cholesterol metabolism disorder. The objective of this study is to determine the impacts of 27-OHC on lysosomal membrane permeabilization (LMP) and pyroptosis in neurons in the development of neural degenerative diseases. Methods: In this study, SH-SY5Y cells and C6 cells were co-cultured in vitro to investigate the influence of 27-OHC on the function of lysosome, lysosomal membrane permeabilization (LMP) and pyroptosis related factors in neuron. Lyso Tracker Red was used to detect the changes of lysosome pH, volume and number. Acridine Orange (AO) Staining was also used to detect the LMP in neurons. Then the morphological changes of cells were observed by scanning electron microscope. The content of lysosome function associated proteins (including Cathepsin B, Cathepsin D, LAMP-1, LAMP-2) and the pyroptosis associated proteins (including NLRP3, GSDMD, caspase-1and IL-1β) were detected through Western blot. Results: Results showed higher levels of lysosome function associated proteins, such as Cathepsin B (p < 0.05), Cathepsin D (p < 0.05), LAMP-1 (p < 0.01), LAMP-2) (p < 0.01) in 27-OHC treated group than that in control group. AO Staining and Lyso-Tracker Red Staining showed that 27-OHC induced lysosome dysfunction with LMP. Content of pyroptosis related factor proteins, such as GSDMD (p < 0.01), NLRP3 (p < 0.001), caspase-1 (p < 0.01) and IL-1β (p < 0.01), were increased in 27-OHC treated neurons. Additionally, cathepsin B was leaked through LMP into the cytosol and induced pyroptosis. Results from the present study also suggested that the CTSB is involved in activation of pyroptosis. Conclusions: Our data indicate that 27-OHC contributes to the pathogenesis of cell death by inducing LMP and pyroptosis in neurons." @default.
• W2920778523 created "2019-03-11" @default.
• W2920778523 creator A5008636823 @default.
• W2920778523 creator A5018863416 @default.
• W2920778523 creator A5030283501 @default.
• W2920778523 creator A5030458072 @default.
• W2920778523 creator A5036505752 @default.
• W2920778523 creator A5047446019 @default.
• W2920778523 creator A5051936772 @default.
• W2920778523 creator A5070445320 @default.
• W2920778523 creator A5080715110 @default.
• W2920778523 date "2019-03-01" @default.
• W2920778523 modified "2023-10-15" @default.
• W2920778523 title "27-Hydroxycholesterol Contributes to Lysosomal Membrane Permeabilization-Mediated Pyroptosis in Co-cultured SH-SY5Y Cells and C6 Cells" @default.
• W2920778523 cites W1010077035 @default.
• W2920778523 cites W121387658 @default.
• W2920778523 cites W1864325817 @default.
• W2920778523 cites W1972483153 @default.
• W2920778523 cites W1982959887 @default.
• W2920778523 cites W2002301299 @default.
• W2920778523 cites W2010628196 @default.
• W2920778523 cites W2027390173 @default.
• W2920778523 cites W2037627674 @default.
• W2920778523 cites W2039184324 @default.
• W2920778523 cites W2053139703 @default.
• W2920778523 cites W2054071408 @default.
• W2920778523 cites W2055998713 @default.
• W2920778523 cites W2061263039 @default.
• W2920778523 cites W2061858464 @default.
• W2920778523 cites W2069274491 @default.
• W2920778523 cites W2080878773 @default.
• W2920778523 cites W2083719092 @default.
• W2920778523 cites W2101472229 @default.
• W2920778523 cites W2113839377 @default.
• W2920778523 cites W2118918019 @default.
• W2920778523 cites W2120302366 @default.
• W2920778523 cites W2152462290 @default.
• W2920778523 cites W2157815025 @default.
• W2920778523 cites W2175794645 @default.
• W2920778523 cites W2180008421 @default.
• W2920778523 cites W218096516 @default.
• W2920778523 cites W2291436232 @default.
• W2920778523 cites W2301103785 @default.
• W2920778523 cites W2306263271 @default.
• W2920778523 cites W2345256317 @default.
• W2920778523 cites W2421416176 @default.
• W2920778523 cites W2510919342 @default.
• W2920778523 cites W2515080674 @default.
• W2920778523 cites W2522054313 @default.
• W2920778523 cites W2534599324 @default.
• W2920778523 cites W2559307267 @default.
• W2920778523 cites W2573995712 @default.
• W2920778523 cites W2584695841 @default.
• W2920778523 cites W2606822554 @default.
• W2920778523 cites W2610948238 @default.
• W2920778523 cites W2627048017 @default.
• W2920778523 cites W2651764022 @default.
• W2920778523 cites W2746736235 @default.
• W2920778523 cites W2751597345 @default.
• W2920778523 cites W2755248898 @default.
• W2920778523 cites W2767533865 @default.
• W2920778523 cites W2770002604 @default.
• W2920778523 cites W2770304782 @default.
• W2920778523 cites W2788640168 @default.
• W2920778523 cites W2790568121 @default.
• W2920778523 cites W2790775254 @default.
• W2920778523 cites W2805481194 @default.
• W2920778523 cites W2898923389 @default.
• W2920778523 cites W4210958114 @default.
• W2920778523 cites W833076889 @default.
• W2920778523 cites W2514822261 @default.
• W2920778523 doi "https://doi.org/10.3389/fnmol.2019.00014" @default.
• W2920778523 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6405519" @default.
• W2920778523 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30881285" @default.
• W2920778523 hasPublicationYear "2019" @default.
• W2920778523 type Work @default.
• W2920778523 sameAs 2920778523 @default.
• W2920778523 citedByCount "37" @default.
• W2920778523 countsByYear W29207785232019 @default.
• W2920778523 countsByYear W29207785232020 @default.
• W2920778523 countsByYear W29207785232021 @default.
• W2920778523 countsByYear W29207785232022 @default.
• W2920778523 countsByYear W29207785232023 @default.
• W2920778523 crossrefType "journal-article" @default.
• W2920778523 hasAuthorship W2920778523A5008636823 @default.
• W2920778523 hasAuthorship W2920778523A5018863416 @default.
• W2920778523 hasAuthorship W2920778523A5030283501 @default.
• W2920778523 hasAuthorship W2920778523A5030458072 @default.
• W2920778523 hasAuthorship W2920778523A5036505752 @default.
• W2920778523 hasAuthorship W2920778523A5047446019 @default.
• W2920778523 hasAuthorship W2920778523A5051936772 @default.
• W2920778523 hasAuthorship W2920778523A5070445320 @default.
• W2920778523 hasAuthorship W2920778523A5080715110 @default.
• W2920778523 hasBestOaLocation W29207785231 @default.
• W2920778523 hasConcept C153911025 @default.
• W2920778523 hasConcept C17172800 @default.
• W2920778523 hasConcept C173633252 @default.
• W2920778523 hasConcept C181199279 @default.
• W2920778523 hasConcept C185592680 @default.
• W2920778523 hasConcept C190283241 @default.

• next