FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2920795461> ?p ?o ?g. }

• W2920795461 endingPage "5338" @default.
• W2920795461 startingPage "5331" @default.
• W2920795461 abstract "RNAs are emerging as important biomarkers and therapeutic targets. The strategy of directly targeting double-stranded RNA (dsRNA) by triplex-formation is relatively underexplored mainly due to the weak binding at physiological conditions for the traditional triplex-forming oligonucleotides (TFOs). Compared to DNA and RNA, peptide nucleic acids (PNAs) are chemically stable and have a neutral peptide-like backbone, and thus, they show significantly enhanced binding to natural nucleic acids. We have successfully developed nucleobase-modified dsRNA-binding PNAs (dbPNAs) to facilitate structure-specific and selective recognition of dsRNA over single-stranded RNA (ssRNA) and dsDNA regions at near-physiological conditions. The triplex formation strategy facilitates the targeting of not only the sequence but also the secondary structure of RNA. Here, we report the development of novel dbPNA-based fluorescent light-up probes through the incorporation of A-U pair-recognizing 5-benzothiophene uracil (btU). The incorporation of btU into dbPNAs does not affect the binding affinity toward dsRNAs significantly, in most cases, as evidenced by our nondenaturing gel shift assay data. The blue fluorescence emission intensity of btU-modified dbPNAs is sequence- and structure-specifically enhanced by dsRNAs, including the influenza viral RNA panhandle duplex and HIV-1–1 ribosomal frameshift-inducing RNA hairpin, but not ssRNAs or DNAs, at 200 mM NaCl, pH 7.5. Thus, dbPNAs incorporating btU-modified and other further modified fluorescent nucleobases will be useful biochemical tools for probing and detecting RNA structures, interactions, and functions." @default.
• W2920795461 created "2019-03-22" @default.
• W2920795461 creator A5011455423 @default.
• W2920795461 creator A5032467527 @default.
• W2920795461 creator A5039250073 @default.
• W2920795461 creator A5040711579 @default.
• W2920795461 creator A5062129951 @default.
• W2920795461 creator A5062382287 @default.
• W2920795461 creator A5062991118 @default.
• W2920795461 creator A5066461716 @default.
• W2920795461 creator A5084610901 @default.
• W2920795461 date "2019-03-15" @default.
• W2920795461 modified "2023-10-15" @default.
• W2920795461 title "Sequence- And Structure-Specific Probing of RNAs by Short Nucleobase-Modified dsRNA-Binding PNAs Incorporating a Fluorescent Light-up Uracil Analog" @default.
• W2920795461 cites W1875625635 @default.
• W2920795461 cites W1968000756 @default.
• W2920795461 cites W1968355807 @default.
• W2920795461 cites W1968421165 @default.
• W2920795461 cites W1980494339 @default.
• W2920795461 cites W1983347833 @default.
• W2920795461 cites W1986588909 @default.
• W2920795461 cites W1989249011 @default.
• W2920795461 cites W1991734736 @default.
• W2920795461 cites W2006167464 @default.
• W2920795461 cites W2009765991 @default.
• W2920795461 cites W2010430643 @default.
• W2920795461 cites W2012006084 @default.
• W2920795461 cites W2018822137 @default.
• W2920795461 cites W2019273806 @default.
• W2920795461 cites W2021865492 @default.
• W2920795461 cites W2027263767 @default.
• W2920795461 cites W2028280716 @default.
• W2920795461 cites W2032136448 @default.
• W2920795461 cites W2032694270 @default.
• W2920795461 cites W2035110007 @default.
• W2920795461 cites W2037017264 @default.
• W2920795461 cites W2040778984 @default.
• W2920795461 cites W2048691199 @default.
• W2920795461 cites W2048791248 @default.
• W2920795461 cites W2048911142 @default.
• W2920795461 cites W2057666290 @default.
• W2920795461 cites W2067763881 @default.
• W2920795461 cites W2068205741 @default.
• W2920795461 cites W2077327925 @default.
• W2920795461 cites W2079377204 @default.
• W2920795461 cites W2081886031 @default.
• W2920795461 cites W2087784483 @default.
• W2920795461 cites W2089571336 @default.
• W2920795461 cites W2096608756 @default.
• W2920795461 cites W2099053774 @default.
• W2920795461 cites W2101219438 @default.
• W2920795461 cites W2105684348 @default.
• W2920795461 cites W2111804964 @default.
• W2920795461 cites W2112757080 @default.
• W2920795461 cites W2128876908 @default.
• W2920795461 cites W2133798407 @default.
• W2920795461 cites W2134166196 @default.
• W2920795461 cites W2141232134 @default.
• W2920795461 cites W2142056356 @default.
• W2920795461 cites W2145334235 @default.
• W2920795461 cites W2147253833 @default.
• W2920795461 cites W2148959513 @default.
• W2920795461 cites W2160057385 @default.
• W2920795461 cites W2163916041 @default.
• W2920795461 cites W2164922887 @default.
• W2920795461 cites W2168036745 @default.
• W2920795461 cites W2226259383 @default.
• W2920795461 cites W2236738108 @default.
• W2920795461 cites W2263000113 @default.
• W2920795461 cites W2314431251 @default.
• W2920795461 cites W2395804252 @default.
• W2920795461 cites W2396853212 @default.
• W2920795461 cites W2397889286 @default.
• W2920795461 cites W2409709309 @default.
• W2920795461 cites W2466965536 @default.
• W2920795461 cites W2478892848 @default.
• W2920795461 cites W2503125643 @default.
• W2920795461 cites W2517866739 @default.
• W2920795461 cites W2531506955 @default.
• W2920795461 cites W2556252626 @default.
• W2920795461 cites W2603187159 @default.
• W2920795461 cites W2618538917 @default.
• W2920795461 cites W2757211226 @default.
• W2920795461 cites W2766432068 @default.
• W2920795461 cites W2767283947 @default.
• W2920795461 cites W2767578311 @default.
• W2920795461 cites W2771932281 @default.
• W2920795461 cites W2786624865 @default.
• W2920795461 cites W2791586881 @default.
• W2920795461 cites W2795531081 @default.
• W2920795461 cites W2883574975 @default.
• W2920795461 cites W2884838339 @default.
• W2920795461 cites W2899224111 @default.
• W2920795461 cites W2902239578 @default.
• W2920795461 cites W2913916873 @default.
• W2920795461 doi "https://doi.org/10.1021/acs.analchem.9b00280" @default.
• W2920795461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30873827" @default.
• W2920795461 hasPublicationYear "2019" @default.

• next