Matches in SemOpenAlex for { <https://semopenalex.org/work/W2920912892> ?p ?o ?g. }
- W2920912892 abstract "In the field of quantitative proteomics, the Isobaric Tags for Relative & Absolute Quantitation (iTRAQ) technology has demonstrated efficacy for proteome monitoring despite its lack of a consensus for data handling. In this study, after peptide and protein identification, we compared the widespread quantitation method based on the calculation of MS/MS reporter ion peaks areas ratios (ProteinPilot) to the alternative method based on the calculation of ratios of the sum of peak intensities (jTRAQx (Quant)) and we processed output data with the in-house Customizable iTRAQ Ratios Calculator (CiR-C) algorithm. Quantitation based on peak area ratios displayed no significant linear correlation with Western blot quantitation. In contrast, quantitation based on the sum of peak intensities displayed a significant linear association with Western blot quantitation (non-zero slope; Pearson correlation coefficient test, r = 0.2962, p = 0.0099 **) with an average bias of 0.08747 ± 0.5004 and 95% Limits of Agreement from - 0.8932 to 1.068. We proposed the Mascot-jTRAQx-CiR-C strategy as a simple yet powerful data processing adjunct to the iTRAQ technology." @default.
- W2920912892 created "2019-03-22" @default.
- W2920912892 creator A5003712720 @default.
- W2920912892 creator A5011796944 @default.
- W2920912892 creator A5020892389 @default.
- W2920912892 creator A5024365801 @default.
- W2920912892 creator A5073480980 @default.
- W2920912892 creator A5076938715 @default.
- W2920912892 creator A5086621234 @default.
- W2920912892 creator A5091575574 @default.
- W2920912892 date "2019-03-13" @default.
- W2920912892 modified "2023-10-17" @default.
- W2920912892 title "Strategies based on sum of peak intensities may better reflect protein expression than peak area integration in iTRAQ protein expression measurement by LC-MS/MS using a TripleTOF 5600+ platform" @default.
- W2920912892 cites W1971676830 @default.
- W2920912892 cites W1973243635 @default.
- W2920912892 cites W1981601285 @default.
- W2920912892 cites W1993991012 @default.
- W2920912892 cites W1999556153 @default.
- W2920912892 cites W2001387260 @default.
- W2920912892 cites W2002294758 @default.
- W2920912892 cites W2015651974 @default.
- W2920912892 cites W2015795623 @default.
- W2920912892 cites W2016022557 @default.
- W2920912892 cites W2016267237 @default.
- W2920912892 cites W2025510888 @default.
- W2920912892 cites W2028211858 @default.
- W2920912892 cites W2032921320 @default.
- W2920912892 cites W2044914821 @default.
- W2920912892 cites W2048678109 @default.
- W2920912892 cites W2062693438 @default.
- W2920912892 cites W2063546126 @default.
- W2920912892 cites W2070689312 @default.
- W2920912892 cites W2071146787 @default.
- W2920912892 cites W2073352191 @default.
- W2920912892 cites W2081945763 @default.
- W2920912892 cites W2095577394 @default.
- W2920912892 cites W2100584122 @default.
- W2920912892 cites W2111208057 @default.
- W2920912892 cites W2117327695 @default.
- W2920912892 cites W2120016245 @default.
- W2920912892 cites W2128969781 @default.
- W2920912892 cites W2133750711 @default.
- W2920912892 cites W2135581618 @default.
- W2920912892 cites W2139208986 @default.
- W2920912892 cites W2140946052 @default.
- W2920912892 cites W2145820743 @default.
- W2920912892 cites W2150981032 @default.
- W2920912892 cites W2167816339 @default.
- W2920912892 cites W2537132940 @default.
- W2920912892 cites W2546452030 @default.
- W2920912892 cites W2553427010 @default.
- W2920912892 cites W2589838140 @default.
- W2920912892 doi "https://doi.org/10.1101/577254" @default.
- W2920912892 hasPublicationYear "2019" @default.
- W2920912892 type Work @default.
- W2920912892 sameAs 2920912892 @default.
- W2920912892 citedByCount "1" @default.
- W2920912892 countsByYear W29209128922021 @default.
- W2920912892 crossrefType "posted-content" @default.
- W2920912892 hasAuthorship W2920912892A5003712720 @default.
- W2920912892 hasAuthorship W2920912892A5011796944 @default.
- W2920912892 hasAuthorship W2920912892A5020892389 @default.
- W2920912892 hasAuthorship W2920912892A5024365801 @default.
- W2920912892 hasAuthorship W2920912892A5073480980 @default.
- W2920912892 hasAuthorship W2920912892A5076938715 @default.
- W2920912892 hasAuthorship W2920912892A5086621234 @default.
- W2920912892 hasAuthorship W2920912892A5091575574 @default.
- W2920912892 hasBestOaLocation W29209128921 @default.
- W2920912892 hasConcept C104317684 @default.
- W2920912892 hasConcept C104397665 @default.
- W2920912892 hasConcept C105795698 @default.
- W2920912892 hasConcept C113196181 @default.
- W2920912892 hasConcept C121332964 @default.
- W2920912892 hasConcept C146211579 @default.
- W2920912892 hasConcept C184185385 @default.
- W2920912892 hasConcept C185592680 @default.
- W2920912892 hasConcept C2776415932 @default.
- W2920912892 hasConcept C2780092901 @default.
- W2920912892 hasConcept C2989005 @default.
- W2920912892 hasConcept C2989041947 @default.
- W2920912892 hasConcept C3018577593 @default.
- W2920912892 hasConcept C33923547 @default.
- W2920912892 hasConcept C43617362 @default.
- W2920912892 hasConcept C46111723 @default.
- W2920912892 hasConcept C55078378 @default.
- W2920912892 hasConcept C55493867 @default.
- W2920912892 hasConcept C6856738 @default.
- W2920912892 hasConcept C71924100 @default.
- W2920912892 hasConcept C80311884 @default.
- W2920912892 hasConcept C89838059 @default.
- W2920912892 hasConcept C97355855 @default.
- W2920912892 hasConceptScore W2920912892C104317684 @default.
- W2920912892 hasConceptScore W2920912892C104397665 @default.
- W2920912892 hasConceptScore W2920912892C105795698 @default.
- W2920912892 hasConceptScore W2920912892C113196181 @default.
- W2920912892 hasConceptScore W2920912892C121332964 @default.
- W2920912892 hasConceptScore W2920912892C146211579 @default.
- W2920912892 hasConceptScore W2920912892C184185385 @default.
- W2920912892 hasConceptScore W2920912892C185592680 @default.
- W2920912892 hasConceptScore W2920912892C2776415932 @default.