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• W2921080611 abstract "Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes." @default.
• W2921080611 created "2019-03-22" @default.
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• W2921080611 date "2019-03-13" @default.
• W2921080611 modified "2023-10-17" @default.
• W2921080611 title "124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics" @default.
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• W2921080611 doi "https://doi.org/10.1021/acs.nanolett.9b00508" @default.
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