FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2921080785> ?p ?o ?g. }

• W2921080785 endingPage "758" @default.
• W2921080785 startingPage "742" @default.
• W2921080785 abstract "Mesenchymal stem cells (MSCs) hold a great promise for successful development of regenerative medicine. Among the plenty of uncovered MSCs sources, desquamated endometrium collected from the menstrual blood probably remains the most accessible. Though numerous studies have been published on human endometrium-derived mesenchymal stem cells (hMESCs) properties in the past years, there are only a few data regarding their genetic modulation. Moreover, there is a lack of information about the fate of the transduced hMESCs. The present study aimed to optimize hMESCs transduction parameters and apply Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology for genome and secretome modification. The fate of hMESCs transduced either in presence of polybrene (Pb) or protamine sulfate (Ps) was assessed by alterations in CD expression profile, growth rate, cell size, migration capability, osteogenic, adipogenic, and decidual differential potentials. Here, we postulated that the use of Ps for hMESCs genetic manipulations is preferable, as it has no impact on the stem-cell properties, whereas Pb application is undesirable, as it induces cellular senescence. Plasminogen activator inhibitor-1 was selected for further targeted hMESCs genome and secretome modification using CRISPR/Cas9 systems. The obtained data provide optimized transduction scheme for hMESCs and verification of its effectiveness by successful hMESCs genome editing via CRISPR/Cas9 technology." @default.
• W2921080785 created "2019-03-22" @default.
• W2921080785 creator A5035659070 @default.
• W2921080785 creator A5046105863 @default.
• W2921080785 creator A5052738306 @default.
• W2921080785 creator A5072869764 @default.
• W2921080785 creator A5077963098 @default.
• W2921080785 creator A5091059718 @default.
• W2921080785 date "2019-03-28" @default.
• W2921080785 modified "2023-10-17" @default.
• W2921080785 title "Optimization of lentiviral transduction parameters and its application for CRISPR-based secretome modification of human endometrial mesenchymal stem cells" @default.
• W2921080785 cites W1524529935 @default.
• W2921080785 cites W1548000125 @default.
• W2921080785 cites W1739944755 @default.
• W2921080785 cites W1973260880 @default.
• W2921080785 cites W1981660632 @default.
• W2921080785 cites W1982080361 @default.
• W2921080785 cites W1983182526 @default.
• W2921080785 cites W1985119800 @default.
• W2921080785 cites W1992760653 @default.
• W2921080785 cites W2007783345 @default.
• W2921080785 cites W2013074450 @default.
• W2921080785 cites W2013357297 @default.
• W2921080785 cites W2016792838 @default.
• W2921080785 cites W2024073909 @default.
• W2921080785 cites W2027831318 @default.
• W2921080785 cites W2031211461 @default.
• W2921080785 cites W2032697503 @default.
• W2921080785 cites W2035788028 @default.
• W2921080785 cites W2036363776 @default.
• W2921080785 cites W2038916787 @default.
• W2921080785 cites W2044459868 @default.
• W2921080785 cites W2048644480 @default.
• W2921080785 cites W2051013988 @default.
• W2921080785 cites W2070334783 @default.
• W2921080785 cites W2071760903 @default.
• W2921080785 cites W2072755256 @default.
• W2921080785 cites W2086181200 @default.
• W2921080785 cites W2109160378 @default.
• W2921080785 cites W2118462479 @default.
• W2921080785 cites W2119975890 @default.
• W2921080785 cites W2120749461 @default.
• W2921080785 cites W2127862779 @default.
• W2921080785 cites W2130201056 @default.
• W2921080785 cites W2136798617 @default.
• W2921080785 cites W2140368652 @default.
• W2921080785 cites W2150890673 @default.
• W2921080785 cites W2151245068 @default.
• W2921080785 cites W2151901676 @default.
• W2921080785 cites W2155732511 @default.
• W2921080785 cites W2164217401 @default.
• W2921080785 cites W2174849886 @default.
• W2921080785 cites W2175410088 @default.
• W2921080785 cites W2259306818 @default.
• W2921080785 cites W2294114319 @default.
• W2921080785 cites W2304917549 @default.
• W2921080785 cites W2331417303 @default.
• W2921080785 cites W2345349969 @default.
• W2921080785 cites W2347092228 @default.
• W2921080785 cites W2397438111 @default.
• W2921080785 cites W2403845114 @default.
• W2921080785 cites W2479719846 @default.
• W2921080785 cites W2480308563 @default.
• W2921080785 cites W2492425651 @default.
• W2921080785 cites W2494515430 @default.
• W2921080785 cites W2505967049 @default.
• W2921080785 cites W2521025961 @default.
• W2921080785 cites W2555145417 @default.
• W2921080785 cites W2592666263 @default.
• W2921080785 cites W2601885162 @default.
• W2921080785 cites W2605187236 @default.
• W2921080785 cites W2605492484 @default.
• W2921080785 cites W2608676324 @default.
• W2921080785 cites W2741653375 @default.
• W2921080785 cites W2746710261 @default.
• W2921080785 cites W2749639510 @default.
• W2921080785 cites W2760127932 @default.
• W2921080785 cites W2770574911 @default.
• W2921080785 doi "https://doi.org/10.1080/15384101.2019.1593650" @default.
• W2921080785 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6464586" @default.
• W2921080785 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30880567" @default.
• W2921080785 hasPublicationYear "2019" @default.
• W2921080785 type Work @default.
• W2921080785 sameAs 2921080785 @default.
• W2921080785 citedByCount "12" @default.
• W2921080785 countsByYear W29210807852019 @default.
• W2921080785 countsByYear W29210807852020 @default.
• W2921080785 countsByYear W29210807852021 @default.
• W2921080785 countsByYear W29210807852022 @default.
• W2921080785 countsByYear W29210807852023 @default.
• W2921080785 crossrefType "journal-article" @default.
• W2921080785 hasAuthorship W2921080785A5035659070 @default.
• W2921080785 hasAuthorship W2921080785A5046105863 @default.
• W2921080785 hasAuthorship W2921080785A5052738306 @default.
• W2921080785 hasAuthorship W2921080785A5072869764 @default.
• W2921080785 hasAuthorship W2921080785A5077963098 @default.
• W2921080785 hasAuthorship W2921080785A5091059718 @default.
• W2921080785 hasBestOaLocation W29210807851 @default.

• next