Matches in SemOpenAlex for { <https://semopenalex.org/work/W2921088517> ?p ?o ?g. }
- W2921088517 endingPage "536" @default.
- W2921088517 startingPage "526" @default.
- W2921088517 abstract "In this work, a novel thermosensitive surface protein imprinted polymer monolithic column (TsIPMC) was synthesized by combining high internal phase emulsion with 1,1-diphenylethene (DPE) controlled polymerization. Innovatively, DPE and acrylic acid (AA) monomers were introduced in high internal oil and water phases respectively. The research showed that DPE could not only initiate the polymerization of monomers, but also improve the pore performance of monolithic columns. The elution efficiency of template or target protein could be significantly improved by the thermoresponse characteristics of TsIPMC. The effects of DPE and AA on adsorption capacity and imprinting factor (IF) were studied. The optimization results presented that the optimal addition amounts were 55 mg and 50 mg. Under such conditions, the IF of as-prepared TsIPMC was 1.61 and the saturated adsorption capacity was 66 mg/mL. The influences of the flow rate and target protein concentration on the adsorption equilibrium time and effluent volume were revealed. TsIPMC showed higher selectivity for different competing proteins. The reuse stability result showed that the adsorption of TsIPMC to BSA decreased by 3.69% after 12 times of reuse, and the IF remained basically unchanged. TsIPMC would demonstrate the potential applications in the field of protein purification and separation." @default.
- W2921088517 created "2019-03-22" @default.
- W2921088517 creator A5026704485 @default.
- W2921088517 creator A5027009250 @default.
- W2921088517 creator A5028494415 @default.
- W2921088517 creator A5030625728 @default.
- W2921088517 creator A5061120009 @default.
- W2921088517 creator A5061758260 @default.
- W2921088517 creator A5075458376 @default.
- W2921088517 creator A5077880590 @default.
- W2921088517 date "2019-08-01" @default.
- W2921088517 modified "2023-09-30" @default.
- W2921088517 title "Preparation of surface protein imprinted thermosensitive polymer monolithic column and its specific adsorption for BSA" @default.
- W2921088517 cites W1574943361 @default.
- W2921088517 cites W1966709166 @default.
- W2921088517 cites W1967554108 @default.
- W2921088517 cites W1976805011 @default.
- W2921088517 cites W1980376401 @default.
- W2921088517 cites W1982709113 @default.
- W2921088517 cites W2010468151 @default.
- W2921088517 cites W2017700328 @default.
- W2921088517 cites W2042748079 @default.
- W2921088517 cites W2043162076 @default.
- W2921088517 cites W2062635777 @default.
- W2921088517 cites W2083449773 @default.
- W2921088517 cites W2087938237 @default.
- W2921088517 cites W2092408986 @default.
- W2921088517 cites W2139065414 @default.
- W2921088517 cites W2141926421 @default.
- W2921088517 cites W2180688687 @default.
- W2921088517 cites W2233888737 @default.
- W2921088517 cites W2277619431 @default.
- W2921088517 cites W2287086718 @default.
- W2921088517 cites W2289629300 @default.
- W2921088517 cites W2493924958 @default.
- W2921088517 cites W2533925470 @default.
- W2921088517 cites W2549622488 @default.
- W2921088517 cites W2564734256 @default.
- W2921088517 cites W2568817473 @default.
- W2921088517 cites W2570576713 @default.
- W2921088517 cites W2576569317 @default.
- W2921088517 cites W2579967479 @default.
- W2921088517 cites W2602741652 @default.
- W2921088517 cites W2739115540 @default.
- W2921088517 cites W2747516038 @default.
- W2921088517 cites W2749942660 @default.
- W2921088517 cites W2772779874 @default.
- W2921088517 cites W2793883339 @default.
- W2921088517 cites W2804232349 @default.
- W2921088517 cites W2809516763 @default.
- W2921088517 cites W2890369577 @default.
- W2921088517 cites W313038786 @default.
- W2921088517 doi "https://doi.org/10.1016/j.talanta.2019.03.056" @default.
- W2921088517 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31036219" @default.
- W2921088517 hasPublicationYear "2019" @default.
- W2921088517 type Work @default.
- W2921088517 sameAs 2921088517 @default.
- W2921088517 citedByCount "32" @default.
- W2921088517 countsByYear W29210885172019 @default.
- W2921088517 countsByYear W29210885172020 @default.
- W2921088517 countsByYear W29210885172021 @default.
- W2921088517 countsByYear W29210885172022 @default.
- W2921088517 countsByYear W29210885172023 @default.
- W2921088517 crossrefType "journal-article" @default.
- W2921088517 hasAuthorship W2921088517A5026704485 @default.
- W2921088517 hasAuthorship W2921088517A5027009250 @default.
- W2921088517 hasAuthorship W2921088517A5028494415 @default.
- W2921088517 hasAuthorship W2921088517A5030625728 @default.
- W2921088517 hasAuthorship W2921088517A5061120009 @default.
- W2921088517 hasAuthorship W2921088517A5061758260 @default.
- W2921088517 hasAuthorship W2921088517A5075458376 @default.
- W2921088517 hasAuthorship W2921088517A5077880590 @default.
- W2921088517 hasConcept C118792377 @default.
- W2921088517 hasConcept C127413603 @default.
- W2921088517 hasConcept C134871310 @default.
- W2921088517 hasConcept C150394285 @default.
- W2921088517 hasConcept C161790260 @default.
- W2921088517 hasConcept C162602672 @default.
- W2921088517 hasConcept C166940927 @default.
- W2921088517 hasConcept C178790620 @default.
- W2921088517 hasConcept C179998833 @default.
- W2921088517 hasConcept C185592680 @default.
- W2921088517 hasConcept C2778123984 @default.
- W2921088517 hasConcept C2780503075 @default.
- W2921088517 hasConcept C39442485 @default.
- W2921088517 hasConcept C42360764 @default.
- W2921088517 hasConcept C43617362 @default.
- W2921088517 hasConcept C44228677 @default.
- W2921088517 hasConcept C521977710 @default.
- W2921088517 hasConcept C73826308 @default.
- W2921088517 hasConcept C88001094 @default.
- W2921088517 hasConceptScore W2921088517C118792377 @default.
- W2921088517 hasConceptScore W2921088517C127413603 @default.
- W2921088517 hasConceptScore W2921088517C134871310 @default.
- W2921088517 hasConceptScore W2921088517C150394285 @default.
- W2921088517 hasConceptScore W2921088517C161790260 @default.
- W2921088517 hasConceptScore W2921088517C162602672 @default.
- W2921088517 hasConceptScore W2921088517C166940927 @default.