Matches in SemOpenAlex for { <https://semopenalex.org/work/W2921619055> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2921619055 endingPage "132" @default.
- W2921619055 startingPage "125" @default.
- W2921619055 abstract "Cryopreservation is an in vitro conservation method which has become an important tool for long-term storage of plant genetic resources. New protocorm-like bodies (PLBs) (about 4-5 mm in diameter) of Cymbidium finlaysonianum Lindl. were isolated individually from 2-month-old proliferating PLB clusters which had been cultured in VW liquid medium (VW; Vacin and Went, 1949) supplemented with 8.84 µM 6-benzyl-aminopurine were successfully cryopreserved using a vitrification method. In this cryogenic procedure, PLBs were precultured in MS liquid medium (MS; Murashige and Skoog, 1962) supplemented with 0.5 M sucrose at 25±2°C for 2 d on an orbital shaker at 110 rpm. The PLBs were treated with loading solution (2 M glycerol plus 0.4 M sucrose) for 20 min at 25±2°C to make the precultured PLBs tolerant to plant vitrification solution 2 (PVS2). Subsequently, the selected PLBs were subjected to PVS2 (30% (w/v) glycerol, 15% (w/v) ethylene glycol, 15% (w/v) dimethyl sulfoxide and 0.4 M sucrose in MS medium, pH 5.8) treatment at various exposure times (0-120 min) at 0°C and plunged into liquid nitrogen (LN) for 1 d. After storage in LN, the PLBs were rewarmed and washed by MS liquid medium containing 0.5 mL of 1.2 M sucrose for 20 min. One week after rewarming PLBs, viability was determined by TTC reduction and regrowth assessed. The results showed that the PLBs precultured with 0.5 M sucrose for 2 d, followed by dehydration with vitrification solution for 60 min had the highest post rewarming viability in terms of TTC reduction (40%) and regrowth (33.5%). No survival rate of PLBs was found without vitrification treatment. Regenerated plants showed the same morphological characteristics as the control." @default.
- W2921619055 created "2019-03-22" @default.
- W2921619055 creator A5018812784 @default.
- W2921619055 creator A5041236473 @default.
- W2921619055 creator A5047994955 @default.
- W2921619055 creator A5074104035 @default.
- W2921619055 creator A5077963889 @default.
- W2921619055 creator A5082799897 @default.
- W2921619055 date "2019-02-01" @default.
- W2921619055 modified "2023-10-14" @default.
- W2921619055 title "Cryopreservation of an endangered pharmaceutically important orchid, <i>Cymbidium finlaysonianum</i> Lindl. using vitrification technique" @default.
- W2921619055 cites W148528149 @default.
- W2921619055 cites W1985412443 @default.
- W2921619055 cites W2000121682 @default.
- W2921619055 cites W2001259815 @default.
- W2921619055 cites W2009306401 @default.
- W2921619055 cites W2011390829 @default.
- W2921619055 cites W2014475231 @default.
- W2921619055 cites W2016512941 @default.
- W2921619055 cites W2022513837 @default.
- W2921619055 cites W2023029287 @default.
- W2921619055 cites W2024556021 @default.
- W2921619055 cites W2040651077 @default.
- W2921619055 cites W2041506075 @default.
- W2921619055 cites W2043993053 @default.
- W2921619055 cites W2044287636 @default.
- W2921619055 cites W2044860740 @default.
- W2921619055 cites W2047450302 @default.
- W2921619055 cites W2048372345 @default.
- W2921619055 cites W2050128839 @default.
- W2921619055 cites W2063923182 @default.
- W2921619055 cites W2071055146 @default.
- W2921619055 cites W2083686851 @default.
- W2921619055 cites W2143139062 @default.
- W2921619055 cites W2461208511 @default.
- W2921619055 cites W249387915 @default.
- W2921619055 cites W2591293133 @default.
- W2921619055 cites W2737148285 @default.
- W2921619055 doi "https://doi.org/10.17660/actahortic.2019.1234.16" @default.
- W2921619055 hasPublicationYear "2019" @default.
- W2921619055 type Work @default.
- W2921619055 sameAs 2921619055 @default.
- W2921619055 citedByCount "2" @default.
- W2921619055 countsByYear W29216190552021 @default.
- W2921619055 countsByYear W29216190552022 @default.
- W2921619055 crossrefType "journal-article" @default.
- W2921619055 hasAuthorship W2921619055A5018812784 @default.
- W2921619055 hasAuthorship W2921619055A5041236473 @default.
- W2921619055 hasAuthorship W2921619055A5047994955 @default.
- W2921619055 hasAuthorship W2921619055A5074104035 @default.
- W2921619055 hasAuthorship W2921619055A5077963889 @default.
- W2921619055 hasAuthorship W2921619055A5082799897 @default.
- W2921619055 hasConcept C16685009 @default.
- W2921619055 hasConcept C179345059 @default.
- W2921619055 hasConcept C179933525 @default.
- W2921619055 hasConcept C185933670 @default.
- W2921619055 hasConcept C18903297 @default.
- W2921619055 hasConcept C196843134 @default.
- W2921619055 hasConcept C2775935240 @default.
- W2921619055 hasConcept C2778022349 @default.
- W2921619055 hasConcept C505870484 @default.
- W2921619055 hasConcept C59822182 @default.
- W2921619055 hasConcept C71924100 @default.
- W2921619055 hasConcept C86803240 @default.
- W2921619055 hasConceptScore W2921619055C16685009 @default.
- W2921619055 hasConceptScore W2921619055C179345059 @default.
- W2921619055 hasConceptScore W2921619055C179933525 @default.
- W2921619055 hasConceptScore W2921619055C185933670 @default.
- W2921619055 hasConceptScore W2921619055C18903297 @default.
- W2921619055 hasConceptScore W2921619055C196843134 @default.
- W2921619055 hasConceptScore W2921619055C2775935240 @default.
- W2921619055 hasConceptScore W2921619055C2778022349 @default.
- W2921619055 hasConceptScore W2921619055C505870484 @default.
- W2921619055 hasConceptScore W2921619055C59822182 @default.
- W2921619055 hasConceptScore W2921619055C71924100 @default.
- W2921619055 hasConceptScore W2921619055C86803240 @default.
- W2921619055 hasIssue "1234" @default.
- W2921619055 hasLocation W29216190551 @default.
- W2921619055 hasOpenAccess W2921619055 @default.
- W2921619055 hasPrimaryLocation W29216190551 @default.
- W2921619055 hasRelatedWork W1544113648 @default.
- W2921619055 hasRelatedWork W1972917209 @default.
- W2921619055 hasRelatedWork W2023558490 @default.
- W2921619055 hasRelatedWork W2151029822 @default.
- W2921619055 hasRelatedWork W2157153554 @default.
- W2921619055 hasRelatedWork W2382639178 @default.
- W2921619055 hasRelatedWork W2913737711 @default.
- W2921619055 hasRelatedWork W2921619055 @default.
- W2921619055 hasRelatedWork W3195128277 @default.
- W2921619055 hasRelatedWork W4220983607 @default.
- W2921619055 isParatext "false" @default.
- W2921619055 isRetracted "false" @default.
- W2921619055 magId "2921619055" @default.
- W2921619055 workType "article" @default.