FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2922004616> ?p ?o ?g. }

• W2922004616 endingPage "875" @default.
• W2922004616 startingPage "866" @default.
• W2922004616 abstract "Primary metabolism plays a key role in the synthesis of secondary metabolite. In this study, the main transcription factors in carbon, nitrogen, and phosphorus metabolisms (CcpA, CcpC, CcpN, CodY, TnrA, GlnR, and PhoP) were engineered to improve bacitracin yield in Bacillus licheniformis DW2, an industrial strain for bacitracin production. First, our results demonstrated that deletions of ccpC and ccpN improved ATP and NADPH supplies, and the bacitracin yields were respectively increased by 14.02% and 16.06% compared with that of DW2, while it was decreased significantly in ccpA deficient strain DW2ΔccpA. Second, excessive branched chain amino acids (BCAAs) were accumulated in codY, tnrA, and glnR deletion strains DW2ΔcodY, DW2ΔtnrA, and DW2ΔglnR, which resulted in the nitrogen catabolite repressions and reductions of bacitracin yields. Moreover, overexpression of these regulators improved intracellular BCAA supplies, and further enhanced bacitracin yields by 14.17%, 12.98%, and 16.20%, respectively. Furthermore, our results confirmed that phosphate addition reduced bacitracin synthesis capability, and bacitracin yield was improved by 15.71% in gene phop deletion strain. On the contrary, overexpression of PhoP led to a 19.40% decrease of bacitracin yield. Finally, a combinatorial engineering of these above metabolic manipulations was applied, and bacitracin yield produced by the final strain DW2-CNCTGP (Simultaneously deleting ccpC, ccpN, phop and overexpressing glnR, codY, and tnrA in DW2) reached 1014.38 U/mL, increased by 35.72% compared to DW2, and this yield was the highest bacitracin yield currently reported. Taken together, this study implied that metabolic engineering of carbon, nitrogen, and phosphorus metabolism regulators is an efficient strategy to enhance bacitracin production, and provided a promising B. licheniformis strain for industrial production of bacitracin." @default.
• W2922004616 created "2019-03-22" @default.
• W2922004616 creator A5003576654 @default.
• W2922004616 creator A5029700598 @default.
• W2922004616 creator A5032057273 @default.
• W2922004616 creator A5042328954 @default.
• W2922004616 creator A5054814478 @default.
• W2922004616 creator A5065827653 @default.
• W2922004616 creator A5079338602 @default.
• W2922004616 creator A5080882875 @default.
• W2922004616 creator A5084959027 @default.
• W2922004616 date "2019-03-13" @default.
• W2922004616 modified "2023-10-14" @default.
• W2922004616 title "Metabolic Engineering of Main Transcription Factors in Carbon, Nitrogen, and Phosphorus Metabolisms for Enhanced Production of Bacitracin in <i>Bacillus licheniformis</i>" @default.
• W2922004616 cites W138657883 @default.
• W2922004616 cites W1515163878 @default.
• W2922004616 cites W2001872144 @default.
• W2922004616 cites W2014663246 @default.
• W2922004616 cites W2017140888 @default.
• W2922004616 cites W2025046721 @default.
• W2922004616 cites W2030164703 @default.
• W2922004616 cites W2036778321 @default.
• W2922004616 cites W2039833881 @default.
• W2922004616 cites W2039947832 @default.
• W2922004616 cites W2047517716 @default.
• W2922004616 cites W2048352856 @default.
• W2922004616 cites W2055509346 @default.
• W2922004616 cites W2065688292 @default.
• W2922004616 cites W2108480548 @default.
• W2922004616 cites W2109918485 @default.
• W2922004616 cites W2117875874 @default.
• W2922004616 cites W2145478799 @default.
• W2922004616 cites W2156438853 @default.
• W2922004616 cites W2195409253 @default.
• W2922004616 cites W2226140769 @default.
• W2922004616 cites W2297141882 @default.
• W2922004616 cites W2346633676 @default.
• W2922004616 cites W2405163886 @default.
• W2922004616 cites W2499377586 @default.
• W2922004616 cites W2520424726 @default.
• W2922004616 cites W2546307091 @default.
• W2922004616 cites W2553043432 @default.
• W2922004616 cites W2563357854 @default.
• W2922004616 cites W2576161661 @default.
• W2922004616 cites W2591471572 @default.
• W2922004616 cites W2592597012 @default.
• W2922004616 cites W2594400616 @default.
• W2922004616 cites W2604059586 @default.
• W2922004616 cites W2608199690 @default.
• W2922004616 cites W2608246063 @default.
• W2922004616 cites W2622558856 @default.
• W2922004616 cites W2623710582 @default.
• W2922004616 cites W2624099204 @default.
• W2922004616 cites W2743323335 @default.
• W2922004616 cites W2747638128 @default.
• W2922004616 cites W2771343120 @default.
• W2922004616 cites W2783755818 @default.
• W2922004616 cites W2793520355 @default.
• W2922004616 cites W2801460589 @default.
• W2922004616 cites W2808696648 @default.
• W2922004616 cites W2810178608 @default.
• W2922004616 cites W2819386545 @default.
• W2922004616 cites W2894132914 @default.
• W2922004616 cites W2899301487 @default.
• W2922004616 doi "https://doi.org/10.1021/acssynbio.9b00005" @default.
• W2922004616 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30865822" @default.
• W2922004616 hasPublicationYear "2019" @default.
• W2922004616 type Work @default.
• W2922004616 sameAs 2922004616 @default.
• W2922004616 citedByCount "44" @default.
• W2922004616 countsByYear W29220046162019 @default.
• W2922004616 countsByYear W29220046162020 @default.
• W2922004616 countsByYear W29220046162021 @default.
• W2922004616 countsByYear W29220046162022 @default.
• W2922004616 countsByYear W29220046162023 @default.
• W2922004616 crossrefType "journal-article" @default.
• W2922004616 hasAuthorship W2922004616A5003576654 @default.
• W2922004616 hasAuthorship W2922004616A5029700598 @default.
• W2922004616 hasAuthorship W2922004616A5032057273 @default.
• W2922004616 hasAuthorship W2922004616A5042328954 @default.
• W2922004616 hasAuthorship W2922004616A5054814478 @default.
• W2922004616 hasAuthorship W2922004616A5065827653 @default.
• W2922004616 hasAuthorship W2922004616A5079338602 @default.
• W2922004616 hasAuthorship W2922004616A5080882875 @default.
• W2922004616 hasAuthorship W2922004616A5084959027 @default.
• W2922004616 hasConcept C104317684 @default.
• W2922004616 hasConcept C134121241 @default.
• W2922004616 hasConcept C143065580 @default.
• W2922004616 hasConcept C185592680 @default.
• W2922004616 hasConcept C191897082 @default.
• W2922004616 hasConcept C192562407 @default.
• W2922004616 hasConcept C2776527165 @default.
• W2922004616 hasConcept C2777272437 @default.
• W2922004616 hasConcept C2779860364 @default.
• W2922004616 hasConcept C2781225136 @default.
• W2922004616 hasConcept C501593827 @default.
• W2922004616 hasConcept C523546767 @default.
• W2922004616 hasConcept C54355233 @default.

• next