Matches in SemOpenAlex for { <https://semopenalex.org/work/W2922154557> ?p ?o ?g. }
- W2922154557 endingPage "67" @default.
- W2922154557 startingPage "67" @default.
- W2922154557 abstract "Nitrate reductase (NR) is important for higher land plants, as it catalyzes the rate-limiting step in the nitrate assimilation pathway, the two-electron reduction of nitrate to nitrite. Furthermore, it is considered to be a major enzymatic source of the important signaling molecule nitric oxide (NO), that is produced in a one-electron reduction of nitrite. Like many other plants, the model plant Arabidopsis thaliana expresses two isoforms of NR (NIA1 and NIA2). Up to now, only NIA2 has been the focus of detailed biochemical studies, while NIA1 awaits biochemical characterization. In this study, we have expressed and purified functional fragments of NIA1 and subjected them to various biochemical assays for comparison with the corresponding NIA2-fragments. We analyzed the kinetic parameters in multiple steady-state assays using nitrate or nitrite as substrate and measured either substrate consumption (nitrate or nitrite) or product formation (NO). Our results show that NIA1 is the more efficient nitrite reductase while NIA2 exhibits higher nitrate reductase activity, which supports the hypothesis that the isoforms have special functions in the plant. Furthermore, we successfully restored the physiological electron transfer pathway of NR using reduced nicotinamide adenine dinucleotide (NADH) and nitrate or nitrite as substrates by mixing the N-and C-terminal fragments of NR, thus, opening up new possibilities to study NR activity, regulation and structure." @default.
- W2922154557 created "2019-03-22" @default.
- W2922154557 creator A5032592436 @default.
- W2922154557 creator A5045542176 @default.
- W2922154557 creator A5068803594 @default.
- W2922154557 date "2019-03-16" @default.
- W2922154557 modified "2023-10-16" @default.
- W2922154557 title "Isoform-Specific NO Synthesis by Arabidopsis thaliana Nitrate Reductase" @default.
- W2922154557 cites W1523282979 @default.
- W2922154557 cites W1566740190 @default.
- W2922154557 cites W1719776338 @default.
- W2922154557 cites W1976210830 @default.
- W2922154557 cites W1976706472 @default.
- W2922154557 cites W1979963390 @default.
- W2922154557 cites W1979965401 @default.
- W2922154557 cites W1981530365 @default.
- W2922154557 cites W1981678617 @default.
- W2922154557 cites W1982444952 @default.
- W2922154557 cites W1983135138 @default.
- W2922154557 cites W1984535511 @default.
- W2922154557 cites W1991337086 @default.
- W2922154557 cites W1994002838 @default.
- W2922154557 cites W2000516639 @default.
- W2922154557 cites W2002664168 @default.
- W2922154557 cites W2003247260 @default.
- W2922154557 cites W2004160418 @default.
- W2922154557 cites W2006075724 @default.
- W2922154557 cites W2015247835 @default.
- W2922154557 cites W2016958628 @default.
- W2922154557 cites W2018289835 @default.
- W2922154557 cites W2019176353 @default.
- W2922154557 cites W2022295793 @default.
- W2922154557 cites W2036703358 @default.
- W2922154557 cites W2038529781 @default.
- W2922154557 cites W2045593769 @default.
- W2922154557 cites W2046363884 @default.
- W2922154557 cites W2047824908 @default.
- W2922154557 cites W2048909444 @default.
- W2922154557 cites W2049782298 @default.
- W2922154557 cites W2065680103 @default.
- W2922154557 cites W2073816818 @default.
- W2922154557 cites W2082217325 @default.
- W2922154557 cites W2088730237 @default.
- W2922154557 cites W2095626755 @default.
- W2922154557 cites W2096578215 @default.
- W2922154557 cites W2097653436 @default.
- W2922154557 cites W2100837269 @default.
- W2922154557 cites W2101108802 @default.
- W2922154557 cites W2101159681 @default.
- W2922154557 cites W2101960772 @default.
- W2922154557 cites W2109283541 @default.
- W2922154557 cites W2116307807 @default.
- W2922154557 cites W2121372562 @default.
- W2922154557 cites W2122901957 @default.
- W2922154557 cites W2126033809 @default.
- W2922154557 cites W2136683403 @default.
- W2922154557 cites W2139274441 @default.
- W2922154557 cites W2140147440 @default.
- W2922154557 cites W2144088584 @default.
- W2922154557 cites W2154687103 @default.
- W2922154557 cites W2165448239 @default.
- W2922154557 cites W2166761952 @default.
- W2922154557 cites W2173332569 @default.
- W2922154557 cites W2206985606 @default.
- W2922154557 cites W2341914988 @default.
- W2922154557 cites W2464922792 @default.
- W2922154557 cites W2566306819 @default.
- W2922154557 cites W2568046757 @default.
- W2922154557 cites W2799730546 @default.
- W2922154557 cites W1974562380 @default.
- W2922154557 doi "https://doi.org/10.3390/plants8030067" @default.
- W2922154557 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6473903" @default.
- W2922154557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30884848" @default.
- W2922154557 hasPublicationYear "2019" @default.
- W2922154557 type Work @default.
- W2922154557 sameAs 2922154557 @default.
- W2922154557 citedByCount "43" @default.
- W2922154557 countsByYear W29221545572019 @default.
- W2922154557 countsByYear W29221545572020 @default.
- W2922154557 countsByYear W29221545572021 @default.
- W2922154557 countsByYear W29221545572022 @default.
- W2922154557 countsByYear W29221545572023 @default.
- W2922154557 crossrefType "journal-article" @default.
- W2922154557 hasAuthorship W2922154557A5032592436 @default.
- W2922154557 hasAuthorship W2922154557A5045542176 @default.
- W2922154557 hasAuthorship W2922154557A5068803594 @default.
- W2922154557 hasBestOaLocation W29221545571 @default.
- W2922154557 hasConcept C104317684 @default.
- W2922154557 hasConcept C143065580 @default.
- W2922154557 hasConcept C178790620 @default.
- W2922154557 hasConcept C181199279 @default.
- W2922154557 hasConcept C185592680 @default.
- W2922154557 hasConcept C2775988993 @default.
- W2922154557 hasConcept C2776179834 @default.
- W2922154557 hasConcept C2776384668 @default.
- W2922154557 hasConcept C2778710519 @default.
- W2922154557 hasConcept C2781341435 @default.
- W2922154557 hasConcept C3119751 @default.