FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2922190717> ?p ?o ?g. }

• W2922190717 abstract "Human adipose stem cells (ASCs) have emerged as a promising treatment paradigm for skin wounds. Recent works demonstrate that the therapeutic effect of stem cells is partially mediated by extracellular vesicles, which comprise exosomes and microvesicles. In this study, we investigate the regenerative effects of isolated microvesicles from ASCs and evaluate the mechanisms how ASC microvesicles promote wound healing. Adipose stem cell-derived microvesicles (ASC-MVs) were isolated by differential ultracentrifugation, stained by PKH26, and characterized by electron microscopy and dynamic light scattering (DLS). We examined ASC-MV effects on proliferation, migration, and angiogenesis of keratinocytes, fibroblasts, and endothelial cells both in vitro and in vivo. Next, we explored the underlying mechanisms by gene expression analysis and the activation levels of AKT and ERK signaling pathways in all three kinds of cells after ASC-MV stimulation. We then assessed the effect of ASC-MVs on collagen deposition, neovascularization, and re-epithelialization in an in vivo skin injury model. ASC-MVs could be readily internalized by human umbilical vein endothelial cells (HUVECs), HaCAT, and fibroblasts and significantly promoted the proliferation, migration, and angiogenesis of these cells both in vitro and in vivo. The gene expression of proliferative markers (cyclin D1, cyclin D2, cyclin A1, cyclin A2) and growth factors (VEGFA, PDGFA, EGF, FGF2) was significantly upregulated after ASC-MV treatment. Importantly, ASC-MVs stimulated the activation of AKT and ERK signaling pathways in those cells. The local injection of ASC-MVs at wound sites significantly increased the re-epithelialization, collagen deposition, and neovascularization and led to accelerated wound closure. Our data suggest that ASC-MVs can stimulate HUVEC, HaCAT, and fibroblast functions. ASC-MV therapy significantly accelerates wound healing, and the benefits of ASC-MVs may due to the involvement of AKT and ERK signaling pathways. This illustrates the therapeutic potential of ASC-MVs which may become a novel treatment paradigm for cutaneous wound healing." @default.
• W2922190717 created "2019-03-22" @default.
• W2922190717 creator A5000901768 @default.
• W2922190717 creator A5009085464 @default.
• W2922190717 creator A5012541451 @default.
• W2922190717 creator A5030123331 @default.
• W2922190717 creator A5035724323 @default.
• W2922190717 creator A5036211071 @default.
• W2922190717 creator A5042192237 @default.
• W2922190717 creator A5042483754 @default.
• W2922190717 creator A5051544202 @default.
• W2922190717 creator A5058363207 @default.
• W2922190717 creator A5061814935 @default.
• W2922190717 creator A5084621356 @default.
• W2922190717 date "2019-01-31" @default.
• W2922190717 modified "2023-10-15" @default.
• W2922190717 title "Microvesicles from human adipose stem cells promote wound healing by optimizing cellular functions via AKT and ERK signaling pathways" @default.
• W2922190717 cites W1600024584 @default.
• W2922190717 cites W1804576389 @default.
• W2922190717 cites W1860690722 @default.
• W2922190717 cites W1991014907 @default.
• W2922190717 cites W1998417918 @default.
• W2922190717 cites W1999417374 @default.
• W2922190717 cites W2011924190 @default.
• W2922190717 cites W2018173663 @default.
• W2922190717 cites W2022397532 @default.
• W2922190717 cites W2033641981 @default.
• W2922190717 cites W2057356848 @default.
• W2922190717 cites W2080418767 @default.
• W2922190717 cites W2085293124 @default.
• W2922190717 cites W2120472250 @default.
• W2922190717 cites W2133465414 @default.
• W2922190717 cites W2143421846 @default.
• W2922190717 cites W2145142940 @default.
• W2922190717 cites W2158217645 @default.
• W2922190717 cites W2311489434 @default.
• W2922190717 cites W2318433546 @default.
• W2922190717 cites W2325727928 @default.
• W2922190717 cites W2330170792 @default.
• W2922190717 cites W2379625445 @default.
• W2922190717 cites W2405091380 @default.
• W2922190717 cites W2441825791 @default.
• W2922190717 cites W2519687050 @default.
• W2922190717 cites W2538912716 @default.
• W2922190717 cites W2563046824 @default.
• W2922190717 cites W2592939174 @default.
• W2922190717 cites W2604300896 @default.
• W2922190717 cites W2610819297 @default.
• W2922190717 cites W2624243169 @default.
• W2922190717 cites W2747589912 @default.
• W2922190717 cites W2767421147 @default.
• W2922190717 cites W2782826313 @default.
• W2922190717 cites W2786202610 @default.
• W2922190717 cites W2786514708 @default.
• W2922190717 cites W2792315201 @default.
• W2922190717 cites W2810276296 @default.
• W2922190717 cites W2883384705 @default.
• W2922190717 doi "https://doi.org/10.1186/s13287-019-1152-x" @default.
• W2922190717 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6357421" @default.
• W2922190717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30704535" @default.
• W2922190717 hasPublicationYear "2019" @default.
• W2922190717 type Work @default.
• W2922190717 sameAs 2922190717 @default.
• W2922190717 citedByCount "165" @default.
• W2922190717 countsByYear W29221907172019 @default.
• W2922190717 countsByYear W29221907172020 @default.
• W2922190717 countsByYear W29221907172021 @default.
• W2922190717 countsByYear W29221907172022 @default.
• W2922190717 countsByYear W29221907172023 @default.
• W2922190717 crossrefType "journal-article" @default.
• W2922190717 hasAuthorship W2922190717A5000901768 @default.
• W2922190717 hasAuthorship W2922190717A5009085464 @default.
• W2922190717 hasAuthorship W2922190717A5012541451 @default.
• W2922190717 hasAuthorship W2922190717A5030123331 @default.
• W2922190717 hasAuthorship W2922190717A5035724323 @default.
• W2922190717 hasAuthorship W2922190717A5036211071 @default.
• W2922190717 hasAuthorship W2922190717A5042192237 @default.
• W2922190717 hasAuthorship W2922190717A5042483754 @default.
• W2922190717 hasAuthorship W2922190717A5051544202 @default.
• W2922190717 hasAuthorship W2922190717A5058363207 @default.
• W2922190717 hasAuthorship W2922190717A5061814935 @default.
• W2922190717 hasAuthorship W2922190717A5084621356 @default.
• W2922190717 hasBestOaLocation W29221907171 @default.
• W2922190717 hasConcept C104317684 @default.
• W2922190717 hasConcept C145059251 @default.
• W2922190717 hasConcept C185592680 @default.
• W2922190717 hasConcept C203014093 @default.
• W2922190717 hasConcept C20518536 @default.
• W2922190717 hasConcept C2780269544 @default.
• W2922190717 hasConcept C2780394083 @default.
• W2922190717 hasConcept C28328180 @default.
• W2922190717 hasConcept C502942594 @default.
• W2922190717 hasConcept C55493867 @default.
• W2922190717 hasConcept C57074206 @default.
• W2922190717 hasConcept C62478195 @default.
• W2922190717 hasConcept C75217442 @default.
• W2922190717 hasConcept C86803240 @default.
• W2922190717 hasConcept C95444343 @default.
• W2922190717 hasConceptScore W2922190717C104317684 @default.
• W2922190717 hasConceptScore W2922190717C145059251 @default.

• next