Matches in SemOpenAlex for { <https://semopenalex.org/work/W2922285188> ?p ?o ?g. }
- W2922285188 endingPage "e110" @default.
- W2922285188 startingPage "e110" @default.
- W2922285188 abstract "Bacterial whole genome sequencing (WGS) is becoming a widely-used technique in research, clinical diagnostic, and public health laboratories. It enables high resolution characterization of bacterial pathogens in terms of properties that include antibiotic resistance, molecular epidemiology, and virulence. The introduction of next-generation sequencing instrumentation has made WGS attainable in terms of costs. However, the lack of a beginner's protocol for WGS still represents a barrier to its adoption in some settings. Here, we present detailed step-by-step methods for obtaining WGS data from a range of different bacteria (Gram-positive, Gram-negative, and acid-fast) using the Illumina platform. Modifications have been performed with respect to DNA extraction and library normalization to maximize the output from the laboratory consumables invested. The protocol represents a simplified and reproducible method for producing high quality sequencing data. The key advantages of this protocol include: simplicity of the protocol for users with no prior genome sequencing experience and reproducibility of the protocol across a wide range of bacteria." @default.
- W2922285188 created "2019-03-22" @default.
- W2922285188 creator A5007386382 @default.
- W2922285188 creator A5058681844 @default.
- W2922285188 creator A5061181173 @default.
- W2922285188 creator A5079000710 @default.
- W2922285188 creator A5082344175 @default.
- W2922285188 date "2019-03-15" @default.
- W2922285188 modified "2023-10-13" @default.
- W2922285188 title "A step-by-step beginner" @default.
- W2922285188 cites W1664588247 @default.
- W2922285188 cites W1863711781 @default.
- W2922285188 cites W1919517754 @default.
- W2922285188 cites W1987093848 @default.
- W2922285188 cites W2051903478 @default.
- W2922285188 cites W2073518110 @default.
- W2922285188 cites W2096565388 @default.
- W2922285188 cites W2098504913 @default.
- W2922285188 cites W2106588460 @default.
- W2922285188 cites W2128880918 @default.
- W2922285188 cites W2133340476 @default.
- W2922285188 cites W2152732832 @default.
- W2922285188 cites W2162767575 @default.
- W2922285188 cites W2179285301 @default.
- W2922285188 cites W2295808945 @default.
- W2922285188 cites W2411811910 @default.
- W2922285188 cites W2464805220 @default.
- W2922285188 cites W2593960886 @default.
- W2922285188 cites W2620298867 @default.
- W2922285188 cites W2793033682 @default.
- W2922285188 cites W2807516929 @default.
- W2922285188 doi "https://doi.org/10.14440/jbm.2019.276" @default.
- W2922285188 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6706130" @default.
- W2922285188 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31453259" @default.
- W2922285188 hasPublicationYear "2019" @default.
- W2922285188 type Work @default.
- W2922285188 sameAs 2922285188 @default.
- W2922285188 citedByCount "22" @default.
- W2922285188 countsByYear W29222851882020 @default.
- W2922285188 countsByYear W29222851882021 @default.
- W2922285188 countsByYear W29222851882022 @default.
- W2922285188 countsByYear W29222851882023 @default.
- W2922285188 crossrefType "journal-article" @default.
- W2922285188 hasAuthorship W2922285188A5007386382 @default.
- W2922285188 hasAuthorship W2922285188A5058681844 @default.
- W2922285188 hasAuthorship W2922285188A5061181173 @default.
- W2922285188 hasAuthorship W2922285188A5079000710 @default.
- W2922285188 hasAuthorship W2922285188A5082344175 @default.
- W2922285188 hasBestOaLocation W29222851881 @default.
- W2922285188 hasConcept C104317684 @default.
- W2922285188 hasConcept C136886441 @default.
- W2922285188 hasConcept C141231307 @default.
- W2922285188 hasConcept C142724271 @default.
- W2922285188 hasConcept C144024400 @default.
- W2922285188 hasConcept C147789679 @default.
- W2922285188 hasConcept C185592680 @default.
- W2922285188 hasConcept C19165224 @default.
- W2922285188 hasConcept C204787440 @default.
- W2922285188 hasConcept C24432333 @default.
- W2922285188 hasConcept C2780385302 @default.
- W2922285188 hasConcept C3742359 @default.
- W2922285188 hasConcept C41008148 @default.
- W2922285188 hasConcept C51679486 @default.
- W2922285188 hasConcept C54355233 @default.
- W2922285188 hasConcept C552990157 @default.
- W2922285188 hasConcept C62126406 @default.
- W2922285188 hasConcept C70721500 @default.
- W2922285188 hasConcept C71924100 @default.
- W2922285188 hasConcept C86803240 @default.
- W2922285188 hasConceptScore W2922285188C104317684 @default.
- W2922285188 hasConceptScore W2922285188C136886441 @default.
- W2922285188 hasConceptScore W2922285188C141231307 @default.
- W2922285188 hasConceptScore W2922285188C142724271 @default.
- W2922285188 hasConceptScore W2922285188C144024400 @default.
- W2922285188 hasConceptScore W2922285188C147789679 @default.
- W2922285188 hasConceptScore W2922285188C185592680 @default.
- W2922285188 hasConceptScore W2922285188C19165224 @default.
- W2922285188 hasConceptScore W2922285188C204787440 @default.
- W2922285188 hasConceptScore W2922285188C24432333 @default.
- W2922285188 hasConceptScore W2922285188C2780385302 @default.
- W2922285188 hasConceptScore W2922285188C3742359 @default.
- W2922285188 hasConceptScore W2922285188C41008148 @default.
- W2922285188 hasConceptScore W2922285188C51679486 @default.
- W2922285188 hasConceptScore W2922285188C54355233 @default.
- W2922285188 hasConceptScore W2922285188C552990157 @default.
- W2922285188 hasConceptScore W2922285188C62126406 @default.
- W2922285188 hasConceptScore W2922285188C70721500 @default.
- W2922285188 hasConceptScore W2922285188C71924100 @default.
- W2922285188 hasConceptScore W2922285188C86803240 @default.
- W2922285188 hasIssue "1" @default.
- W2922285188 hasLocation W29222851881 @default.
- W2922285188 hasLocation W29222851882 @default.
- W2922285188 hasLocation W29222851883 @default.
- W2922285188 hasLocation W29222851884 @default.
- W2922285188 hasLocation W29222851885 @default.
- W2922285188 hasOpenAccess W2922285188 @default.
- W2922285188 hasPrimaryLocation W29222851881 @default.
- W2922285188 hasRelatedWork W1501508789 @default.