Matches in SemOpenAlex for { <https://semopenalex.org/work/W2922367771> ?p ?o ?g. }
- W2922367771 abstract "Due to their ability to present foreign antigens and prime naïve T cells, macrophages and dendritic cells (DCs) are referred to as professional antigen-presenting cells (APCs). Although activated macrophages may function as APCs, these cells are particularly effective at directly engaging pathogens through phagocytosis, and production of antimicrobial compounds. On the other hand, DCs possess superb antigen-presenting and costimulatory capacity and they are essential for commencement and regulation of adaptive immune responses. In in vitro models, development of mature mammalian DCs from monocytes requires sequential exposure to growth factors (including GM-CSF and IL-4) and proinflammatory stimuli such as toll-like receptor (TLR) ligands. Currently, except for IL-4/13, neither orthologues nor functional analogues of the growth factors which are essential for the differentiation of mammalian DCs (including GM-CSF and FLT3) have been identified in teleosts and data about differentiation of piscine APCs is scant. In the present study, primary salmon mononuclear phagocytes (MPs) stimulated in vitro for 5-7 days with a B-class CpG oligodeoxynucleotides (ODN 2006PS) underwent morphological differentiation and developed “dendritic” morphology, characterized by long, branching pseudopodia. Transcriptional profiling showed that these cells expressed high levels of proinflammatory mediators characteristic for M1 polarized MPs. However, the cells treated with CpGs for 7 days downregulated their surface MHCII molecules as well as their capacity to endocytose ovalbumin and exhibited attenuated allostimulatory activity. This concurred with transcriptional downregulation of costimulatory CD80/86 and upregulation of inhibitory CD274 (B7-H1) genes. Despite their exhausted allostimulatory activity, these cells were still responsive to re-stimulation with gardiquimod (a TLR7/8 ligand) and further upregulated a wide array of immune genes including proinflammatory mediators such as intereukin-1 beta and tumor necrosis factor. Overall, the presented data highlight the disparate effects TLR ligands may have on the proinflammatory status of APCs, on one side, and their antigen-presenting/costimulatory functions, on the other. These findings also indicate that despite the poor phylogenetic conservation of the growth factors involved in the differentiation of DCs, some of the processes that orchestrate the development and the differentiation of professional APCs are conserved between teleosts in mammals." @default.
- W2922367771 created "2019-03-22" @default.
- W2922367771 creator A5008435897 @default.
- W2922367771 creator A5019486697 @default.
- W2922367771 creator A5051989846 @default.
- W2922367771 creator A5052159087 @default.
- W2922367771 creator A5061103902 @default.
- W2922367771 creator A5075346685 @default.
- W2922367771 date "2019-03-13" @default.
- W2922367771 modified "2023-10-16" @default.
- W2922367771 title "CpGs Induce Differentiation of Atlantic Salmon Mononuclear Phagocytes Into Cells With Dendritic Morphology and a Proinflammatory Transcriptional Profile but an Exhausted Allostimulatory Activity" @default.
- W2922367771 cites W1512393880 @default.
- W2922367771 cites W1638480194 @default.
- W2922367771 cites W1656644461 @default.
- W2922367771 cites W1837819215 @default.
- W2922367771 cites W1964575258 @default.
- W2922367771 cites W1971800474 @default.
- W2922367771 cites W1972543778 @default.
- W2922367771 cites W2007034700 @default.
- W2922367771 cites W2013970994 @default.
- W2922367771 cites W2016671523 @default.
- W2922367771 cites W2019546595 @default.
- W2922367771 cites W2023863818 @default.
- W2922367771 cites W2024460967 @default.
- W2922367771 cites W2028092080 @default.
- W2922367771 cites W2031283336 @default.
- W2922367771 cites W2031331495 @default.
- W2922367771 cites W2034642161 @default.
- W2922367771 cites W2036285228 @default.
- W2922367771 cites W2043407765 @default.
- W2922367771 cites W2053092352 @default.
- W2922367771 cites W2053782627 @default.
- W2922367771 cites W2062963211 @default.
- W2922367771 cites W2064233919 @default.
- W2922367771 cites W2064314350 @default.
- W2922367771 cites W2069407133 @default.
- W2922367771 cites W2073187732 @default.
- W2922367771 cites W2073220467 @default.
- W2922367771 cites W2074991973 @default.
- W2922367771 cites W2084185832 @default.
- W2922367771 cites W2086941795 @default.
- W2922367771 cites W2096953772 @default.
- W2922367771 cites W2101672858 @default.
- W2922367771 cites W2105270641 @default.
- W2922367771 cites W2108513000 @default.
- W2922367771 cites W2114048032 @default.
- W2922367771 cites W2115267868 @default.
- W2922367771 cites W2119668085 @default.
- W2922367771 cites W2122791726 @default.
- W2922367771 cites W2125153186 @default.
- W2922367771 cites W2131048729 @default.
- W2922367771 cites W2131761283 @default.
- W2922367771 cites W2142328433 @default.
- W2922367771 cites W2145049340 @default.
- W2922367771 cites W2151035991 @default.
- W2922367771 cites W2161354474 @default.
- W2922367771 cites W2166676682 @default.
- W2922367771 cites W2177076048 @default.
- W2922367771 cites W243049598 @default.
- W2922367771 cites W2791878314 @default.
- W2922367771 cites W2794307519 @default.
- W2922367771 cites W2800545870 @default.
- W2922367771 cites W3112223531 @default.
- W2922367771 cites W45332612 @default.
- W2922367771 cites W78744612 @default.
- W2922367771 doi "https://doi.org/10.3389/fimmu.2019.00378" @default.
- W2922367771 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6424866" @default.
- W2922367771 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30918507" @default.
- W2922367771 hasPublicationYear "2019" @default.
- W2922367771 type Work @default.
- W2922367771 sameAs 2922367771 @default.
- W2922367771 citedByCount "8" @default.
- W2922367771 countsByYear W29223677712020 @default.
- W2922367771 countsByYear W29223677712022 @default.
- W2922367771 countsByYear W29223677712023 @default.
- W2922367771 crossrefType "journal-article" @default.
- W2922367771 hasAuthorship W2922367771A5008435897 @default.
- W2922367771 hasAuthorship W2922367771A5019486697 @default.
- W2922367771 hasAuthorship W2922367771A5051989846 @default.
- W2922367771 hasAuthorship W2922367771A5052159087 @default.
- W2922367771 hasAuthorship W2922367771A5061103902 @default.
- W2922367771 hasAuthorship W2922367771A5075346685 @default.
- W2922367771 hasBestOaLocation W29223677711 @default.
- W2922367771 hasConcept C104317684 @default.
- W2922367771 hasConcept C127561419 @default.
- W2922367771 hasConcept C154317977 @default.
- W2922367771 hasConcept C164027704 @default.
- W2922367771 hasConcept C185592680 @default.
- W2922367771 hasConcept C193419808 @default.
- W2922367771 hasConcept C202751555 @default.
- W2922367771 hasConcept C203014093 @default.
- W2922367771 hasConcept C2776090121 @default.
- W2922367771 hasConcept C2776914184 @default.
- W2922367771 hasConcept C2778170410 @default.
- W2922367771 hasConcept C2778297628 @default.
- W2922367771 hasConcept C39347974 @default.
- W2922367771 hasConcept C55493867 @default.
- W2922367771 hasConcept C67662055 @default.
- W2922367771 hasConcept C83464605 @default.
- W2922367771 hasConcept C86803240 @default.